REN Wei,XU Bo,GENG Hui,et al.Molecular Cloning and Expression Analysis of Catalase (PuCAT) Gene in Puccinellia chinampoensis[J].Northern Horticulture,2014,38(20):90-95.
朝鲜碱茅过氧化氢酶基因（PuCAT）的克隆及表达分析
- Title:
- Molecular Cloning and Expression Analysis of Catalase (PuCAT) Gene in Puccinellia chinampoensis
- 文章编号:
- 1001-0009（2014）20-0090-06
- Keywords:
- Puccinellia chinampoensis; CAT; gene clone
- 分类号:
- Q 785
- 文献标志码:
- A
- 摘要:
- 以朝鲜碱茅（Puccinellia chinampoensis）茎叶组织提取的RNA为模板，根据已报道的CAT基因同源序列设计引物，通过RT-PCR法扩增出1个CAT基因的cDNA序列并与其它植物CAT基因进行同源性比对。结果表明：该基因全长1 487 bp，是一个完整开放阅读框，编码含492个氨基酸的蛋白，Genebank登陆号为：HM230827，命名PuCAT；朝鲜碱茅CAT基因的核苷酸和氨基酸序列与大麦、小麦的同源性最高；并对其信号肽、疏水性、跨膜结构、二级结构和主要功能域做了预测。半定量RT-PCR结果表明，PuCAT基因在4种胁迫处理条件下，有着不同的表达规律，总体而言，在朝鲜碱茅的根部和叶片均可以表达，但是在叶片中的表达量要高于在根中的表达。
- Abstract:
- The full length cDNA sequence of CAT gene was cloned from Puccinellia chinampoensis leaves using RT-PCR method,the primers were designed according to the homologous CAT gene sequences of other plant species.The results showed that the nucleotide sequence of the gene was 1 487 bp,containing a complete open reading frame and encoding 492 amino acids,Genebank：HM230827.Nucleotide and amino acid sequence analysis revealed that PuCAT shared high identity with the orthologs from Triticum aestivum and Hordeum vulgare.And its signal peptide,hydrophobicity/hydrophilic,trans-membrane domain,secondary structure and main functional domains were predicted.Semi-quantitative RT-PCR analysis showed that PuCAT expressed in different tissues,but the expression in root was lower,and in leaf much higher.Various elevated levels of PuCAT expression had been detected when exposed to 4 different stress experimental treatments,and the results were not the same.
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备注/Memo
第一作者简介：任伟（1984-），男，博士研究生，助理研究员，现主要从事牧草逆境生理与分子生物学领域等研究工作。E-mail：wren84@163.com.
责任作者：徐安凯（1959-），男，博士，研究员，现主要从事牧草遗传育种与恢复生态学领域等研究工作。E-mail：xuankai0167@163.com.
基金项目：现代农业产业技术体系建设专项资金资助项目（cars-35-02）。
收稿日期：2014-05-27