GAO Tian,LIU Ruixiang,WANG Yixuan,et al.Changes of Superoxide Dismutase Catalase Activity and Malondialdehyde Content in Leaves of Reaumuria soongorica in Ejina Desert[J].Northern Horticulture,2021,(09):60-67.[doi:10.11937/bfyy.20202839]
额济纳荒漠红砂叶片中保护酶活性及丙二醛含量变化
- Title:
- Changes of Superoxide Dismutase Catalase Activity and Malondialdehyde Content in Leaves of Reaumuria soongorica in Ejina Desert
- 文献标志码:
- A
- 摘要:
- 以额济纳荒漠典型红砂（Reaumuria soongorica）群落为研究对象，通过对额济纳旗荒漠盐化环境下红砂自然种群叶片的保护酶活性和丙二醛含量及其影响因子的分析，了解额济纳荒漠条件对红砂生长发育的影响，以期为野生红砂群落的保护和进一步研究提供参考依据。结果表明：在红砂的一个生长季内，随着时间的推移，红砂叶片中超氧化物歧化酶（SOD）活性呈现出逐渐降低的趋势，最高活性为6.65 U?g-1；丙二醛（MDA）含量呈递增趋势，含量变化为0.012~0.046 μmol?g-1FW；过氧化氢酶（CAT）活性表现出先升高后降低的趋势，最低活性为0.002 4 U?g-1?min-1。2年内，红砂叶片中SOD、CAT活性，与平均温度呈极显著正相关（P<0.01），与空气相对湿度呈极显著负相关（P<0.01）；MDA含量则正好相反，与平均温度呈极显著负相关（P<0.01），与空气相对湿度呈极显著正相关（P<0.01）；各土层土壤含水率（SWC）相关性的试验结果表明，SOD活性与SWC（20~100 cm）呈极显著负相关（P<0.01），与SWC（0~20 cm）呈显著负相关（P<0.05）；CAT活性与SWC（20~100 cm）呈极显著正相关（P<0.01）；MDA含量与SWC（0~20 cm）呈极显著正相关（P<0.01），与SWC（40~60 cm）呈极显著负相关（P<0.01）；20~60 cm土层的土壤含水率对红砂叶片中CAT、SOD活性以及MDA含量影响最大。
- Abstract:
- Taking the typical red sand (Reaumuria soongorica) community in Ejina desert as the research object,the change characteristics of CAT,SOD activities and MDA content in red sand and its influencing factors were analyzed,the influences of Ejina desert conditions on the growth and development of red sand were studied,in order to provide a reference for the protection and further research of wild red sand community.The results showed that,in a growing season of red sand,with the passage of time,SOD activity in leaves of red sand showed a decreasing trend,the highest activity was 6.65 U?g-1,MDA content showed an increasing trend,the content changed from 0.012 μmol?g-1FW to 0.046 μmol?g-1FW,CAT activity showed a trend of increasing at first and then decreasing,the lowest activity was 0.002 4 U?g?min-1.In two years,the activity of SOD and CAT in leaves of red sand were significantly positively correlated with air temperature (P<0.01),and negatively correlated with air relative humidity (P<0.01);the content of MDA was just the opposite,and negatively correlated with air temperature (P<0.01),and positively correlated with air relative humidity (P<0.01);the correlation between SWC of each soil layer showed that the activity of SOD and CAT in leaves of red sand were significantly positively correlated with air temperature (P<0.01),and negatively correlated with air relative humidity (P<0.01);SOD activity was negatively correlated with SWC (20-100 cm) (P<0.01),and negatively correlated with SWC (0-20 cm) (P<0.05);CAT activity was positively correlated with SWC (20-100 cm) (P<0.01);MDA content was positively correlated with SWC (0-20 cm) (P<0.01),and negatively correlated with SWC (40-60 cm) (P<0.01);20-60 cm (P<0.01).The activity of CAT,SOD and the content of MDA in leaves of red sand were most affected by the soil moisture content in 20-60 cm soil layer.
参考文献/References:
[1]马剑英,陈发虎,夏敦胜,等.荒漠植物红砂(Reaumuria soongorica)叶片元素和水分含量与土壤因子的关系[J].生态学报,2008,28(3):983-992.[2]童方平,方伟,马履一,等.湿地松优良半同胞家系保护酶活性及脂质过氧化作用对水分胁迫的生理响应[J].中国农学通报,2007,23(8):72-77.[3]徐莲珍,蔡靖,姜在民,等.水分胁迫对3种苗木叶片渗透调节物质与保护酶活性的影响[J].西北林学院学报,2008,23(2):12-16.[4]刘家琼,邱明新,蒲锦春,等.我国荒漠典型超旱生植物:红砂[J].植物学报，1982(5):485-488.[5]周紫鹃,苏培玺,解婷婷,等.不同生境下红砂(Reaumuria soongorica)的生理生化特征及适应性[J].中国沙漠,2014,34(4):1007-1014.[6]BOWLER C,VANMONTAGU M,INZE D.Superoxide dismutase and stress tolerance[J].Annu Rev Plant Mol Biol,1992,43:83-116.[7]刘玉冰,张腾国,李新荣,等.红砂(Reaumuria soongorica)忍耐极度干旱的保护机制:叶片脱落和茎中蔗糖累积[J].中国科学C辑:生命科学,2006(4):328-333.[8]种培芳,李毅,苏世平,等.红砂3个地理种群的光合特性及其影响因素[J].生态学报,2010,30(4):914-922.[9]周生荟,刘玉冰,谭会娟,等.荒漠植物红砂在持续干旱胁迫下的光保护机制研究[J].中国沙漠,2010,30(1):69-73.[10]李园园,刘瑞香.红砂对荒漠盐化土适应特征[J].内蒙古民族大学学报(自然科学版),2014(3):287-292.[11]岑显超.不同品种(类型)楸树苗木对干旱胁迫的生理响应[D].南京:南京林业大学,2008.[12]陈晓娜,高永,赵纳祺,等.羊柴根系对毛乌素盐碱状况的适应性评价[J].西北农林科技大学学报(自然科学版),2017,45(8):89-94.[13]王宝山,赵思齐.干旱对小麦幼苗膜脂过氧化及保护酶的影响[J].山东师大学报(自然科学版),1987(1):29-38.[14]陈由强,叶冰莹,朱锦懋,等.渗透胁迫对花生幼叶活性氧伤害和膜脂过氧化作用的影响[J].中国油料作物学报,2000,22(1):53-56.[15]苏梦云,范铭庆.渗透胁迫和钙处理对杉木幼苗膜脂过氧化及保护酶活性的影响[J].林业科学研究,2000(4):391-396.[16]聂石辉,齐军仓,张海禄,等.PEG6000模拟干旱胁迫对大麦幼苗丙二醛含量及保护酶活性的影响[J].新疆农业科学,2011,48(1):11-17.[17]LIU S M,XU Z W,WANG W Z,et al.A comparison of eddy-covariance and large aperture scintillometer measurements with respect to the energy balance closure problem[J].Hydrology and Earth System Sciences,2001,15(4):1291-1306.[18]高平,杨卫民.防御活性氧保护系统在黑豆抗旱生理中的响应[J].氨基酸和生物资源,2010,32(4):22-26.[19]李兆伟.水稻叶片早衰突变体的糖代谢基因表达与抗氧化生理调控[D].杭州:浙江大学,2014.[20]李霞,阎秀峰,于涛.水分胁迫对黄檗幼苗保护酶活性及脂质过氧化作用的影响[J].应用生态学报,2005,16(12):2353-2356.[21]韩蕊莲,李丽霞,梁宗锁,等.干旱胁迫下沙棘膜脂过氧化保护体系研究[J].西北林学院学报,2002,17(4):1-5.[22]聂磊,刘鸿先,彭少麟.水分胁迫对长期UV-B辐射下柚树苗生理特性的影响[J].植物资源与环境学报,2001,10(3):19-24.[23]种培芳,苏世平,李毅,等.不同地理种源红砂幼苗对PEG胁迫的生理响应[J].草业学报,2013,22(1):183-192.[24]刘冰.基于稳定性同位素技术荒漠植物红砂水分来源的研究[D].呼和浩特:内蒙古农业大学,2017.
相似文献/References:
[1]刘 萍,邵晓琪,丁义峰,等.不同芍药品种花瓣中营养成分含量的比较[J].北方园艺,2014,38(01):151.
LIU Ping,SHAO Xiao-qi,DING Yi-feng,et al.Comparison on Nutritional Components Contents in Petals of Six Peony Varieties[J].Northern Horticulture,2014,38(09):151.
[2]张书玲,刘建凤,尚爱芹.银条根茎膨大过程中生理生化变化的研究[J].北方园艺,2012,36(03):168.
ZHANG Shu-ling,LIU Jian-feng,SHANG Ai-qin.Study on the Change of Physiological-biochemical Changes ofManyflower Betony During Rhizome Intumescence[J].Northern Horticulture,2012,36(09):168.
[3]袁继杰,张志.六个矮牵牛品种生理指标比较分析[J].北方园艺,2012,36(06):67.
YUAN Ji-jie,ZHANG Zhi.Comparative Analysis of Physiological Indices to Six Petunia hybrida Varieties[J].Northern Horticulture,2012,36(09):67.
[4]王庆军,郝兆祥,侯乐峰,等.低温胁迫下三个石榴品种的抗寒性分析[J].北方园艺,2015,39(02):18.[doi:10.11937/bfyy.201502005]
WANG Qing-jun,HAO Zhao-xiang,HOU Le-feng,et al.Analysis on Cold Resistance of Three Pomegranate Cultivars Under Cold Stress[J].Northern Horticulture,2015,39(09):18.[doi:10.11937/bfyy.201502005]
[5]张立恒,李 坤,胡禧熙,等.施入有机物料对连作葡萄生长及叶片酶活性影响[J].北方园艺,2015,39(11):173.[doi:10.11937/bfyy.201511044]
ZHANG Li-heng,LI Kun,HU Xi-xi,et al.Effect of Organic Materials on Growth and Leave Enzymes Activity of Grapevine in Replant Soil[J].Northern Horticulture,2015,39(09):173.[doi:10.11937/bfyy.201511044]
[6]车建美.,刘 波,张 彦l.,等.青枯雷尔氏菌胁迫对烟草植株抗氧化酶系活性的影响[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102046]
CHE Jian-mei.,LIU Bo,ZHANG Yan.,et al.Effects of Avirulent Ralstonia solanacearum Strain on Some Antioxidant Enzymes Activities in Tobacco Plants[J].Northern Horticulture,2011,35(09):0.[doi:10.11937/bfyy.201102046]
[7]王广慧.植物SOD的分子生物学及其在植物抗逆基因m程中的应用进展[J].北方园艺,2011,35(03):0.[doi:10.11937/bfyy.201103069]
WANG Guang Hui.Research Advance on Molecular Biology of Hant’s Sod and Its Applications in Plant Resistance Genetic Engineering[J].Northern Horticulture,2011,35(09):0.[doi:10.11937/bfyy.201103069]
[8]杨 静,李小娟,王 丽,等.外源过氧化物酶对辣椒果实抗氧化酶活性与品质的影响[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108001]
YANG Jing,LI Xiao-juan,WANG Li,et al.Effect of External Peroxidase on the Activities of Antioxidant Enzymes in Pepper[J].Northern Horticulture,2011,35(09):0.[doi:10.11937/bfyy.201108001]
[9]郭笑,苑广信,王曼力,等.番茄超氧化物歧化酶的生物信息学分析[J].北方园艺,2016,40(21):97.[doi:10.11937/bfyy.201621025]
GUO Xiao,YUAN Guangxin,WANG Manli,et al.Bioinformatics Analysis of Solanum lycopersicum Superoxide Dismutase[J].Northern Horticulture,2016,40(09):97.[doi:10.11937/bfyy.201621025]
[10]王静,自红州,李黄维,等.盐胁迫对短花针茅幼苗生长及抗氧化同工酶的影响[J].北方园艺,2018,42(14):109.[doi:10.11937/bfyy.20174088]
WANG Jing,ZI Hongzhou,LI Huangwei,et al.Effects of Salt Stress on Stipa breviflora Seedling and Antioxidant Isozyme[J].Northern Horticulture,2018,42(09):109.[doi:10.11937/bfyy.20174088]
备注/Memo
第一作者简介：高天（1995-），男，硕士研究生，研究方向为水土保持与荒漠化防治。E-mail:981332482@qq.com.基金项目：国家自然科学基金资助项目（31160108,31460124）。收稿日期：2020-07-06