CHEN Ming-xia,LI Xiang-wu,LI Ming-jun,et al.Effect of PP333 Treatment on Growth，Yield and Quality of Virus-free Rehmannia glutinosa f.Hueichingensis (Chan et Sehih) Hsiao Plantlet[J].Northern Horticulture,2014,38(18):171-174.
多效唑对脱毒怀地黄生长发育、产量及品质的影响
- Title:
- Effect of PP333 Treatment on Growth，Yield and Quality of Virus-free Rehmannia glutinosa f.Hueichingensis (Chan et Sehih) Hsiao Plantlet
- 文章编号:
- 1001-0009（2014）18-0171-04
- Keywords:
- Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao virus-free plantlet; PP333; growth and development; yield; quality
- 分类号:
- S 482.8+99
- 文献标志码:
- A
- 摘要:
- 以继代培养的怀地黄脱毒试管苗“85-5”为试材，研究了多效唑不同喷施浓度和处理次数对脱毒怀地黄生长发育、产量和品质的变化规律。结果表明：随着多效唑喷施浓度和处理次数的增加，怀地黄脱毒苗的株高、叶面积和冠幅都呈现逐渐下降的趋势；随着多效唑喷施浓度和次数的增加，怀地黄产量和品质呈现先上升后下降的趋势；多效唑200 mg/L处理2次是最佳喷施浓度和喷施次数，此时脱毒怀地黄产量最高，为5 682.67 kg/667m2；梓醇和毛蕊花糖苷含量较大，分别为8.33 mg/g和0.55 mg/g。
- Abstract:
- Taking successive transfer culture of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao“85-5” as material,the effect of different concentrations and frequency of PP333 treatment on the growth，yield and quality of virus-free plantlet of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao were studied.The results showed that plant height，leaf area and growth decreased gradually as concentrations and frequency of PP333 treatment increased.As concentration and frequency of CPPU treatment increased，yield and quality of virus-free plantlet of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao increased first then decreased.PP333200 mg/L spring twice was the best concentration of CPPU treatment and spraying frequency，the yield and quality was the best at the same time.The highest yield，content of catalopl and verbascoside were 5 682.67 kg/667m2，8.33 mg/g and 0.55 mg/g respectively.
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备注/Memo
第一作者简介：陈明霞（1973-），女，河南南阳人，博士，副教授，现主要从事植物组织培养教学及药用植物生物技术应用等研究工作。E-mail：chenmx1973@gmail.com.
责任作者：李明军（1962-），男，河南温县人，博士，教授，现主要从事植物生理学教学及植物生物技术应用等研究工作。E-mail：limingjun2002@263.net.
基金项目：河南省科技创新杰出人才计划资助项目（114200510013）；河南省教育厅科技研究重点资助项目（13A180521）；博士启动基金资助项目（01046500020）；大学生创新资助项目（20120221）。
收稿日期：2014-05-27