ZHAO Wei-wei,LI Ai-min,ZHANG Zheng-hai,et al.Study on Extraction Process and Content of Salicylic Acid from Rubus cratacgifolius[J].Northern Horticulture,2014,38(16):127-130.
蓬蘽悬钩子水杨酸提取工艺及含量变化研究
- Title:
- Study on Extraction Process and Content of Salicylic Acid from Rubus cratacgifolius
- 文章编号:
- 1001-0009(2014)16-0127-04
- Keywords:
- Rubus cratacgifolius; salicylic acid; extraction process
- 分类号:
- S663.2
- 文献标志码:
- A
- 摘要:
- 以蓬蘽悬钩子为试材，通过正交实验，对蓬蘽悬钩子中游离态与结合态水杨酸的提取工艺条件进行了优化，并测定了蓬蘽悬钩子生长期各器官中水杨酸含量。结果表明：游离态水杨酸提取最佳工艺条件为以乙腈为提取溶剂，加入磷酸，超声15 min；结合态水杨酸提取最佳工艺条件乙醚作为提取溶剂，加入磷酸，超声15 min。蓬蘽悬钩子新梢和果实中游离态与结合态水杨酸含量变化趋势相似（r=0.868**和0.986**）。开花期前，新梢水杨酸含量较高（1.179 mg/g）；开花期至果实发育期（青果期），新梢水杨酸含量呈下降趋势；果实着色期至充分成熟期，新梢水杨酸含量呈上升趋势；8月上中旬果实采摘后，新梢水杨酸含量急剧上升（1.434 mg/g）。总之，果实水杨酸含量在整个发育期呈下降趋势，发育期（青果期）果实水杨酸含量比成熟期高。
- Abstract:
- Taking Rubus cratacgifolius as material,the extraction conditions of the free and bound salicylic acid in Rubus cratacgifolius were optimized by orthogonal test.The results showed that the best extraction conditions for free salicylic acid were:acetonitrile as the extraction solvent and adding phosphate,ultrasound 15 min;for bound salicylic acid were diehyl ether as the extraction solvent and adding phosphate,ultrasound 15 min.The free and bound salicylic acid displayed similar trends in Rubus cratacgifolius shoots or fruits (r=0.868**and 0.986**);the salicylic acid content of new shoots shown a higher levels (1.179 mg/g) before flowering,and declined gradually from flowering period to fruit development period (Chinese white olive period),increased gradually from coloring to fully mature period,and a sharp rise after picking the fruit in mid-August (1.434 mg/g).In a word,the salicylic acid content of fruit decreased in the whole growth period,and showed higher level than mature period.
参考文献/References:
［1］Paterson J,Baxter G,Lawrence J,et al.Is there a role for dietary salicylates in health［J］.Proceedings of the Nutrition Society,2006，65:93-96.
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备注/Memo
第一作者简介：赵伟伟（1987-），男，硕士，现主要从事药用植物资源等研究工作。E-mail：hkdzww@163.com.