[1]方华舟.不同蛹虫草优质母种分离方式与菌种性能比较[J].北方园艺,2014,38(15):142-145,146.
　FANG Hua-zhou.Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris[J].Northern Horticulture,2014,38(15):142-145,146.

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不同蛹虫草优质母种分离方式与菌种性能比较

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年15期页码: 142-145,146 栏目: 出版日期: 2014-08-15

Title:: Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris

文章编号:: 1001-0009(2014)15-0142-05

作者:: 方华舟; 荆楚理工学院生物工程学院，湖北荆门 448000

Author(s):: FANG Hua-zhou; Institute of Biological Engineering,Jingchu Science and Technology College,Jingmen,Hubei 448000

关键词:: 蛹虫草; 组织分离法; 孢子培养法; 菌种; 培养特征; 产量

Keywords:: Cordyceps militaris; tissue separation; spore cultivation; strain; cultivation characteristics; yield

分类号:: S646

文献标志码:: A

摘要:: 以蛹虫草优质母株为母本，以组织分离法和孢子培养法制备子代菌种为试验组，以优质原始母种为对照，通过比较观察各试验组菌种培养性能及栽培试验结果，探索优质菌种适宜制备方法。结果表明：组织分离法优良菌种率可达85%，高于孢子培养法约30%；同时孢子培养法可产生一定比例性能更优菌株。说明组织分离法更适合于遗传和保持母本性状，孢子培养法尤其适合筛选更优菌株，均可作为优质蛹虫草菌种制备的重要方法之一。

Abstract:: Taking the Cordyceps militaris quality maternal as female parent，the progeny strains prepared from tissue separation and spore cultivation as test group and the original stock culture as control group，the rejuvenation methods of Cordyceps militaris were determined by comparing the test results of strain characteristics and cultivation.The results showed that the tissue isolation fine strains rate of 85%,was 30% higher than the spore culture method,spore culture method could produce a certain proportion of better strains.It proved that tissue isolation method was more suitable for the genetic and maternal character,spore culture method was especially suitable for screening better strains,both as a fine strain of Cordyceps militaris rejuvenation method.

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备注/Memo

作者简介：方华舟（1965-），男，湖北罗田人，副教授，研究方向为食用菌与农业微生物学。E-mail:fanghuazhou2000@sina.com.

基金项目：湖北省教育厅重点科研资助项目(D 20126101)。

更新日期/Last Update: 2014-09-21