XU Dong-sheng.Study on Extraction Total Flavonoids by Microwave From Dioscorea opposita[J].Northern Horticulture,2014,38(15):139-141.
微波提取山药黄酮的方法研究
- Title:
- Study on Extraction Total Flavonoids by Microwave From Dioscorea opposita
- 文章编号:
- 1001-0009(2014)15-0139-03
- Keywords:
- Dioscorea opposita; microwave; flavonoid
- 分类号:
- S632.1
- 文献标志码:
- A
- 摘要:
- 以山药为试材，用微波消解仪提取黄酮，分光光度法测定黄酮含量，采用单因子试验及正交实验方法研究山药黄酮的最佳提取条件。结果表明：微波提取山药黄酮最适条件是乙醇浓度60%，提取温度95℃，料液比1∶20 g/mL，提取时间20 min，黄酮提取率达0.428%,各因子对提取效果影响的大小顺序是微波温度>料液比>乙醇浓度，提取时间对黄酮提取率影响不大。
- Abstract:
- Using the Dioscorea opposita as material and ethanol as solvent to extract total flavonoids with microwave digestion instrument,the content of total flavonoids was determined by spectrophotometry,the optimal condition of extraction was studied through the orthogonal test based on the single factor.The results showed the optimal extraction conditions were as follows：ethanol concentration 60％,temperature 95℃,material to liquid ratio 1∶20 g/mL,extraction time 20 min,flavonoids extraction rate was 0.428%.The order of affecting was the extraction temperature>material to liquid ratio>ethanol concentration,the affecting of extraction time was no significant difference.
参考文献/References:
［1］姜红波.山药的药理活性研究及产品开发现状［J］.化学与生物工程,2011,28(4):9-12.
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备注/Memo
作者简介：徐东生（1955-），男，湖北武汉人，高级实验师，现主要从事植物方面的研究工作。E-mail:1377994531@qq.com.