[1]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106-108.
　LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(01):106-108.
点击复制

黄山贡菊茎尖脱毒快繁技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年01期 页码: 106-108 栏目: 出版日期: 2014-01-10
Title:
Tissue Culture and Rapid Propagation of Chrysanthemum morifolium
文章编号:
1001-0009（2014）01-0106-03
作者:
李云玲1; 孙 虎1; 苗锦山1; 刘 杰1; 孙明茂1; 孙海亮2
1.潍坊科技学院 园艺科学与技术研究所，山东 寿光 262700;
2.山东禾宜生物科技公司，山东 寿光 262700
Author(s):
LI Yun-ling1; SUN Hu1; MIAO Jin-shan1; LIU Jie1; SUN Ming-mao1; SUN Hai-liang2
1.Institute of Horticultural Science and Technology,Weifang University of Science and Technology,Shouguang，Shandong 262700;
2.Shandong Heyi Bio-technology Corporation，Shouguang,Shandong 262700
关键词:
黄山贡菊; 茎尖; 组织培养; 快繁
Keywords:
Chrysanthemum morifolium; stem tip; tissue culture; rapid propagation
分类号:
S 682.1+1
文献标志码:
A
摘要:
以黄山贡菊茎段为外植体，研究了不同消毒方法和不同培养基对黄山贡菊茎尖组织培养的影响。结果表明：0.1%升汞消毒8 min，外植体污染率及褐化率较低；诱导茎尖分化的最适培养基为MS+6-BA 1.0 mg/L +NAA 0.05 mg/L，芽诱导率可高达100%，且丛生芽生长较快；最适宜生根的培养基为MS+NAA 0.5 mg/L，植株生根粗壮，根系发达，利于练苗及移栽。
Abstract:
With the stem tips of Chrysanthemum morifoliumas the explants,the effects of different disinfection methods and culture mediums on stem tip tissue culture were studied.The results showed that the contamination rate and browning rate of the explants were the lowest by 0.1% mercuric chloride for 8 min.The optimum culture medium for inducing adventitious shoots was MS+6-BA 1.0 mg/L +NAA 0.05 mg/L,the bud induction rate was up to 100%,and the multiple shoots grow rapidly；the optimum rooting culture medium was MS+NAA 0.5 mg/L,it was easier for acclimatizing and transplanting with the sturdy root system.

参考文献/References:

[1] 薛建平,张爱民,赵丰兰.安徽药菊茎尖组织培养技术的研究[J].中国中药杂志,2002,27(5)：350-353.

[2] 蔡祝南,杨莉莉,彭超美,等.切花菊病毒脱毒及脱毒苗的检测[J].植物病理学报,1992,22(1):34.

[3] 宋瑞林,吴如健,黄兆才.福建省菊花病毒病的初步调查与检测[J].福建农业科技,1997(5):17.

[4] 张伟.切花菊组织培养及快速繁殖的初代研究[J].现代园艺,2012(17):4-5.

[5] 刘庆昌,吴国良.植物细胞组织培养[M].北京.中国农业大学出版社,2003:78-79.

[6] 刘丽辉,王亦民,方顺生.黄山贡菊的组织培养与快速繁殖[J].植物生理学通讯,2001,37(4):314-315.

[7] 刘丽辉.黄山贡菊的脱毒快繁与栽培技术[J].特产研究,2002(1):48-49.

[8] 薛建平,张爱民,常玮.安徽药菊叶片直接再生植株技术的研究[J].中国中药杂志,2004,29(2):132-134.

[9] 薛建平,常玮,张爱民,等.安徽药菊花瓣组织培养技术的研究[J].中国中药杂志,2004,29(3)：211-214.

[10]代丽萍.药菊组织培养研究进展[J].安徽农学通报,2008,14(7):86-88.


相似文献/References:

[1]杨莉娜,何涛,王海涛.藏药植物川西獐牙菜的快速繁殖技术研究[J].北方园艺,2013,37(13):141.
　YANG Li-na,HE Tao,WANG Hai-tao.Rapid Propagation of Tibetan Medicinal Plant Swertia mussotii Franch[J].Northern Horticulture,2013,37(01):141.
[2]王金录,钤泰琳,赵美爱.青岛百合包埋-玻璃化法超低温保存技术研究[J].北方园艺,2012,36(18):135.
　WANG Jin-lu,QIAN Tai-lin,ZHAO Mei-ai.Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method[J].Northern Horticulture,2012,36(01):135.
[3]梁美霞.连翘茎尖培养与快速繁殖研究[J].北方园艺,2012,36(18):139.
　LIANG Mei-xia.Study on Shoot Tips Culture and Rapid Propagation of Forsythia suspensa[J].Northern Horticulture,2012,36(01):139.
[4]曹冬煦,周海峰,于生成,等.“巨峰”葡萄热处理茎尖培养技术研究[J].北方园艺,2012,36(09):116.
　CAO Dong-xu,ZHOU Hai-feng,YU Sheng-cheng,et al.Study on Heat-treated Stem Tip Culture of Kyoho Grape[J].Northern Horticulture,2012,36(01):116.
[5]宋健坤,黄曼娜,王然.梨砧木OH×F51的离体组织培养研究[J].北方园艺,2014,38(18):107.
　SONG Jian-kun,HUANG Man-na,WANG Ran.Research on in vitro Tissue Culture of Pear Rootstocks OH×F51[J].Northern Horticulture,2014,38(01):107.
[6]董敬超.“红颜”草莓茎尖组织培养快繁技术研究[J].北方园艺,2013,37(24):106.
　DONG Jing-chao.Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe’[J].Northern Horticulture,2013,37(01):106.
[7]王建书,孙松松,刘浩,等.西葫芦茎尖离体培养和快速繁殖技术[J].北方园艺,2015,39(22):96.[doi:10.11937/bfyy.201522025]
　WANG Jianshu,SUN Songsong,LIU Hao,et al.Induction of Direct Shoot Organogenesis From Shoot Tip Explants of Cucurbita pepo L.[J].Northern Horticulture,2015,39(01):96.[doi:10.11937/bfyy.201522025]
[8]张艳秋,屈连伟,苏君伟,等.菊花茎尖玻璃化法超低温保存技术研究[J].北方园艺,2016,40(05):128.[doi:10.11937/bfyy.201605034]
　ZHANG Yanqiu,QU Lianwei,SU Junwei,et al.Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method[J].Northern Horticulture,2016,40(01):128.[doi:10.11937/bfyy.201605034]
[9]马少梅,麻冬梅,谢应忠,等.北海道黄杨茎尖组织培养初报[J].北方园艺,2010,34(03):0.[doi:10.11937/bfyy.201003035]
　MA Shao-mei,MA Dong-mei,XIE Ying-zhong,et al.Advances on the Establislunent of Shoot-tip of Euonymus japonicus[J].Northern Horticulture,2010,34(01):0.[doi:10.11937/bfyy.201003035]
[10]姚依岑,王子钰,丛天慈,等.无侧枝切花菊组培快繁体系的建立[J].北方园艺,2025,(8):8.[doi:10.11937/bfyy.20245092]
　YAO Yicen,WANG Ziyu,CONG Tianci,et al.Establishment of Efficient and Rapid Propagation System for Tissue Culture of Non-branching Cut Chrysanthemum[J].Northern Horticulture,2025,(01):8.[doi:10.11937/bfyy.20245092]

备注/Memo

第一作者简介：李云玲（1982-），女，硕士，讲师，现主要从事农业生物技术等研究工作。E-mail:liyunling82@126.com.

责任作者：苗锦山（1972-），男，博士，副教授，现主要从事作物育种等研究工作。E-mail:lnmjs@163.com.

更新日期/Last Update: 2014-08-08