LI Wei-fen,QIN Rong,LIU Fa-wan,et al.Study on Physiological Changs of Brassica juncea Coss.Under Drought Stress Simulated by PEG6000[J].Northern Horticulture,2013,37(21):5-8.
芥菜在聚乙二醇模拟干旱条件下的生理变化研究
- Title:
- Study on Physiological Changs of Brassica juncea Coss.Under Drought Stress Simulated by PEG6000
- 文章编号:
- 1001-0009(2013)21-0005-04
- 关键词:
- 芥菜; 苗期; 抗旱性; 聚乙二醇(PEG6000); 模拟干旱胁迫
- Keywords:
- Brassica juncea Coss.; seeding stage; drought resistance; PEG6000; simulated drought stress
- 分类号:
- S 637
- 文献标志码:
- A
- 摘要:
- 以云南地方芥菜品种“M100”(Brassica juncea Coss.)为试材,研究了5%、8%和10%聚乙二醇(PEG6000)渗透胁迫下芥菜幼苗的叶绿素含量、丙二醛(MDA)含量、脯氨酸含量、可溶性糖含量、相对含水量、相对电导率和Fv/Fm等生理指标随胁迫处理时间的变化。结果表明:随着PEG6000胁迫时间的延长或PEG6000浓度的升高,芥菜叶片叶绿素含量、相对含水量、Fv/Fm下降比较明显,MDA含量、脯氨酸含量、可溶性糖含量和相对电导率则表现出不同程度的增加趋势。其中MDA含量、脯氨酸含量、可溶性糖含量、相对含水量和相对电导率5个指标在PEG6000浓度为10%时,各处理间差异显著,因此,这5个指标可用于测定芥菜的耐旱性。
- Abstract:
- Taking Yunnan local mustard variety ‘M100’ (Brassica juncea Coss.) as test material,the physiological changes under PEG6000 stress with the concentrations of 5%,8% and 10% were investigated.Physiological indexes such as the content of chlorophyll,MDA,proline,soluble sugar,relative water content,relative electric conductivity,Fv/Fm were determined with the prolongation of the time of PEG6000-induced stress.The results showed that with the increasing of PEG6000 concentration and the prolongation of treatment time,chlorophyll,relative water content,Fv/Fm of the leaf decreased obviously,and the content of MDA,proline,soluble sucrose,relative electric conductivity increased in varying degrees.Among these indexes,MDA content,proline content,soluble sugar content,relative water content,relative electric conductivity reached a significant difference between treatments under the concentration of 10% PEG6000 simulated drought stress,so these five indexes could be used for determination of mustard drought resistance.
参考文献/References:
[1]黄兴奇.云南作物种质资源(果树、油料、小宗作物、蔬菜篇)[M].昆明:云南科技出版社,2008. [2]康绍忠.新农业科技革命与21世纪我国节水农业的发展[J].干旱地区农业研究,1998,16(1):11-17. [3]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:260-261. [4]祁云枝,杜勇军.干旱胁迫下黄瓜及蚕豆叶片膜透性改变及其机理的初步研究[J].陕西农业科学,1997(4):6-7. [5]蔡小东,郭见林.PEG模拟干旱胁迫下辣椒相关生理指标的变化[J].湖南农业科学,2008(6):49-50,58. [6]李志刚,刘威,林彰文,等.芦笋在PEG模拟干旱条件下的生理生化变化[J].生态科学,2006,25(1):21-23. [7]宋唯一.PEG6000模拟干旱胁迫对黄瓜幼苗生长的影响[J].湖北农业科学,2011,50(12):2394-2397. [8]王荣华,石雷,汤庚国,等.渗透胁迫对蒙古冰草幼苗保护酶系统的影响[J].植物学通报,2003,20(3):330-335. [9]王霞,候平.植物对于旱胁迫的适应机理[J].干旱区研究,2001,18(2):42-46. [10]李德全,郭清福,张以勤,等.冬小麦抗旱性生理特性的研究[J].作物学报,1993,19(2):125-131. [11]任丽花,王义祥,翁伯琦,等.土壤水分胁迫对圆叶决明含水量和光合特性的影响[J].厦门大学学报(增刊),2005,44:28-31. [12]芮仁廉,聂毓琦,童红玉.超氧物歧化酶(SOD)作为甘薯种质源抗逆性参数的探讨[J].江苏农业学报,1990,6(4):52-56. [13]肖用森.在渗透胁迫下稻苗中游离脯氨酸的积累与膜质过氧化的关系[J].武汉植物学研究,1996,14(4):334-340. [14]姚觉,于晓英,邱收,等.植物抗旱机理研究进展[J].华北农学报,2007,22(增刊):51-56. [15]韦小丽,徐锡增,朱守谦.水分胁迫下榆科3种幼苗生理生化指标的变化[J].南京林业大学学报(自然科学版),2005,29(2):47-50.
相似文献/References:
[1]王娟婷,王乃江.辽东栎幼苗的生长规律研究[J].北方园艺,2014,38(08):64.
WANG Juan-ting,WANG Nai-jiang.Research on Seedling Growth Law of Quercus Liaotungensis Koidz[J].Northern Horticulture,2014,38(21):64.
[2]宋敏丽,温祥珍,李亚灵,等.六个番茄品种的耐热性鉴定[J].北方园艺,2012,36(21):5.
SONG Min-li,WEN Xiang-zhen,LI Ya-ling,et al.Heat Tolerance Identification of Six Tomato Varieties[J].Northern Horticulture,2012,36(21):5.
[3]李 莉,郝 丽,热甫开提,等.腐植酸复混肥对新疆温室苗期加工番茄养分吸收和生长的影响[J].北方园艺,2015,39(13):160.[doi:10.11937/bfyy.201513046]
LI Li,HAO Li,Refukaiti,et al.Study on Effect of Applied Humic Acid Compound Fertilizer on Nutrient Absorption and Growth of Greenhouse Seedling Processing Tomatoes in Xinjiang[J].Northern Horticulture,2015,39(21):160.[doi:10.11937/bfyy.201513046]
[4]赵雁,车伟光,毕玉芬.“德钦”紫花苜蓿苗期耐热性综合评价[J].北方园艺,2015,39(20):69.[doi:10.11937/bfyy.201520018]
ZHAO Yan,CHE Weiguang,BI Yufen.Thermotolerance Comprehensive Evaluation of Medicago sativa L.‘Deqin’ on Seedling Stage[J].Northern Horticulture,2015,39(21):69.[doi:10.11937/bfyy.201520018]
[5]高苇,王勇,张春祥.不同黄瓜品种对黄瓜棒孢叶斑病的抗性鉴定[J].北方园艺,2016,40(11):110.[doi:10.11937/bfyy.201611028]
GAO Wei,WANG Yong,ZHANG Chunxiang.Resistance Identification of Different Cucumber Varieties to Corynespora Leaf Spot[J].Northern Horticulture,2016,40(21):110.[doi:10.11937/bfyy.201611028]
[6]吴星,赵虎,王萌,等.广西塑料大棚彩椒套种包心芥菜安全高产栽培技术[J].北方园艺,2016,40(19):204.[doi:10.11937/bfyy.201619052]
[J].Northern Horticulture,2016,40(21):204.[doi:10.11937/bfyy.201619052]
[7]许红娟,陈之林,杨澜,等.五种彩叶矾根苗期在贵州冬季生长适应性比较[J].北方园艺,2016,40(24):61.[doi:10.11937/bfyy.201624016]
XU Hongjuan,CHEN Zhilin,YANG Lan,et al.Winter Adaptability Comparative Analysis on Five Kinds Varieties Coleus(Heuchera micrantha) of Seedling Growth in Guizhou[J].Northern Horticulture,2016,40(21):61.[doi:10.11937/bfyy.201624016]
[8]许红娟,陈之林,罗孝明,等.彩叶矾根幼苗形态生理特性变化及耐阴性综合评价[J].北方园艺,2017,41(15):89.[doi:10.11937/bfyy.20170044]
XU Hongjuan,CHEN Zhilin,LUO Xiaoming,et al.Comprehensive Evaluation on Shade-tolerance of Heuchera micrantha Seedling and Morphological and Physiological Characteristics[J].Northern Horticulture,2017,41(21):89.[doi:10.11937/bfyy.20170044]
[9]张喜斌,秦楚,麻冬梅,等.紫花苜蓿苗期耐盐性鉴定[J].北方园艺,2017,41(22):43.[doi:10.11937/bfyy.20171470]
ZHANG Xibin,QIN Chu,MA Dongmei,et al.Identification of Salt Tolerance of Alfalfa in Seedling Stage[J].Northern Horticulture,2017,41(21):43.[doi:10.11937/bfyy.20171470]
[10]牛玉,刘子记,刘昭华,等.苦瓜种质资源耐冷性鉴定[J].北方园艺,2017,41(24):1.[doi:10.11937/bfyy.20172273]
NIU Yu,LIU Ziji,LIU Zhaohua,et al.Chilling Tolerance Evaluation of Bitter Gourd Germplasm[J].Northern Horticulture,2017,41(21):1.[doi:10.11937/bfyy.20172273]
备注/Memo
第一作者简介:李卫芬(1977-),女,云南丽江人,硕士,助理研究员,现主要从事蔬菜资源开发与利用等研究工作。E-mail:1614242375@qq.com.