YE Zhen-mei.Study on Optimization of Extracting Technology of Polysaccharides from Poria coccus by Response Surface Methodology and Its Immune Activity[J].Northern Horticulture,2013,37(17):135-138.
茯苓多糖的响应面提取条件优化及体外免疫活性研究
- Title:
- Study on Optimization of Extracting Technology of Polysaccharides from Poria coccus by Response Surface Methodology and Its Immune Activity
- 文章编号:
- 1001-0009(2013)17-0135-04
- 分类号:
- S 567.3+2
- 文献标志码:
- A
- 摘要:
- 以茯苓为原料，研究了茯苓多糖的响应面提取条件优化及体外免疫活性。结果表明：茯苓多糖提取最佳工艺条件为微波功率471.3 W，微波提取时间8.5 min，乙醇浓度82.3%，液料比为19.9，在该条件下，茯苓多糖的提取率为2.96%。茯苓多糖具有较好的刺激淋巴细胞增殖的能力，随着茯苓粗多糖浓度的增加，其刺激淋巴细胞增殖的能力也逐渐增强，当茯苓粗多糖浓度超过100 μg/mL时，其刺激淋巴细胞增殖的能力反而下降。
- Abstract:
- Taking Poria coccus as material,the extraction technology of polysaccharides from it was optimized by response surface methodology and its immune activity was studied.The results showed that the optimum extraction conditions of polysaccharides from Poria coccus was microwave power 471.3 W,microwave extraction time 8.5 min,ethanol concentration 82.3%,solution and material ratio 19.9,under these conditions,the polysaccharides yield could reach 2.96%.The polysaccharides from Poria coccus had proliferative effect on leukomonocyte.The proliferative effect strengthened gradually with the concentration of polysaccharides from Poria coccus increased.When its concentration exceeded 100 μg/mL,the proliferative effect decreased.
参考文献/References:
[1] 胡斌,杨益平,叶阳.茯苓化学成分研究[J].中草药,2006(5):12-14.
[2] 刘林,霍志斐,史树堂.茯苓多糖的药理作用概述[J].河北中医,2010(9):23-25.
[3] 邱绿琴,杨惊宇,吴宗彬.茯苓多糖的提取分离方法研究[J].当代医学,2009(3):56-57.
[4] 曲瑾郁,任大明.蛹虫草多糖的化学修饰及体外抗氧化能力[J].食品科学,2011(15):34-35.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(17):133.
[3]罗 琴,林惠昆,管春平,等.正交实验法优化提取二型鳞毛蕨多糖工艺的研究[J].北方园艺,2013,37(22):146.
LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(17):146.
[4]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(17):128.
[5]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(17):156.
[6]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(17):148.
[7]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(17):154.
[8]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(17):166.
[9]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(17):135.
[10]谢银军,吴香梅,张培旗.超高压提取鱼腥草多糖最佳工艺研究[J].北方园艺,2013,37(10):153.
XIE Yin-jun,WU Xiang-mei,ZHANG Pei-qi.Study on Extraction Process Optimum of Polysaccharides from Houttuynia cordata Thunb Using Ultra High Pressure Method[J].Northern Horticulture,2013,37(17):153.
[11]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(17):148.
[12]徐雷,刘常丽,刘合刚,等.不同温度贮藏条件下茯苓品质变化研究[J].北方园艺,2014,38(22):170.
XU Lei,LIU Chang-li,LIU He-gang,et al.Preliminary Research on the Quality Change of Wolfiporia cocos Storaged Under Different Temperature Conditions[J].Northern Horticulture,2014,38(17):170.
备注/Memo
作者简介：叶振梅（1967-），女，硕士，高级讲师，研究方向为妇产科与人体免疫学。
收稿日期：2013-04-10