不同核桃品种的ISSR标记分析
- 摘要:
- 以西北农林科技大学资源苗圃的18个核桃品种嫩叶为试材,利用ISSR分子标记技术对其进行了遗传多样性分析,建立了最适PCR反应体系。结果表明:从31条引物中筛选出多态性强重复性好的15条引物,共检测到132个多态位点,平均每条引物扩增出多态性位点8.8个。"西林3号"和"辽宁4号"的相似系数最大(0.7639),"西洛3号"和"新疆2号"最小(0.2500)。多态信息含量(PIC)变化范围在0.7105～0.9151,其值均大于0.5,说明15条引物扩增的位点均表现出高度多态,同时表明供试样品遗传资源非常丰富。
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[1]刘志曦,李志强,魏海莲,等.平菇ISSR遗传多样性分析[J].北方园艺,2014,38(12):94.
LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(04):94.
[2]李 娜,张存旭,崔晓燕,等.栓皮栎ISSR反应体系的建立[J].北方园艺,2014,38(13):93.
LI Na,ZHANG Cun-xu,CUI Xiao-yan,et al.Establishment of ISSR Reaction System in Quercus variabilis [J].Northern Horticulture,2014,38(04):93.
[3]杨雨华,宗建伟,岳汉秋,等.核桃净光合速率日变化与环境因子的关系[J].北方园艺,2014,38(11):32.
YANG Yu-hua,ZONG Jian-wei,YUE Han-qiu,et al.Relationship Between Diurnal Changes of Net Photosynthetic Rate and Environmental Factors in Leaves of ‘Luguang’Juglans regia L.[J].Northern Horticulture,2014,38(04):32.
[4]刘 枫,赵宝军,宫永红,等.聚乙烯醇保湿核桃高接改造技术[J].北方园艺,2014,38(11):51.
[5]王鸿磊,丁 强,吕 蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014,38(11):96.
WANG Hong-lei,DING qiang,LV Wei,et al.Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas[J].Northern Horticulture,2014,38(04):96.
[6]郭丽霞,翟梅枝,许 静,等.核桃采穗圃母枝修剪方式研究[J].北方园艺,2013,37(22):23.
GUO Li-xia,ZHAI Mei-zhi,XU Jing,et al.Study on Pruning Methods of Base Shoot in Walnut Scion Orchard[J].Northern Horticulture,2013,37(04):23.
[7]李 娜,张存旭,刘 伟,等.毛梾ISSR-PCR反应体系的建立[J].北方园艺,2013,37(22):114.
LI Na,ZHANG Cun-xu,LIU Wei,et al.Establishment of ISSRPCR Reaction System of Cornus walteri[J].Northern Horticulture,2013,37(04):114.
[8]郭明月,孙连海,张臻.红小豆核桃复合植物蛋白饮料的最佳工艺研究[J].北方园艺,2013,37(13):169.
GUO Ming-yue,SUN Lian-hai,ZHANG Zhen.Study on the Optimum Technology of Compound Vegetable Protein Drinks of Adzuki Bean and Walnut[J].Northern Horticulture,2013,37(04):169.
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YANG Zhong-wei,LU Bin,LIU Jin-feng,et al.Breeding of Walnut Variety ‘Shengyong’[J].Northern Horticulture,2014,38(04):152.
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备注/Memo
基金:国家林业公益性行业科研专项资助项目(201004027)