ZHANG Yan,LI Bin,LI Ru-juan,et al.Study on Observation of Aseptic Germination of Bletilla striata Seeds and Rapid Propagation of Bletilla bulbocodioides [J].Northern Horticulture,2013,37(03):158-160.
白芨种子的无菌萌发过程观察和组培快繁研究
- Title:
- Study on Observation of Aseptic Germination of Bletilla striata Seeds and Rapid Propagation of Bletilla bulbocodioides
- 文章编号:
- 1001-0009(2013)03-0158-03
- Keywords:
- Bletilla; aseptic germination; rapid propagation
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以白芨种子为培养材料，对白芨种子无菌非共生萌发过程中原球茎发育进行了研究；并以白芨组培苗的原球茎和叶片为外植体，进行白芨组织培养快速繁殖技术研究。结果表明：在白芨的种子发育过程中，种胚发育成幼苗有2种方式，1种是小球体经原球茎发育成小苗；第2种是小球体伸长成根状茎后发育成小苗。组培苗的原球茎经切割后在诱导培养基上培养，可产生增殖分化，形成丛芽。原球茎诱导的最适培养基为1/2MS+6-BA 5.0 mg/L+NAA 0.2 mg/L，增殖倍数为2.71。以叶片为外植体，未获得组培苗。
- Abstract:
- Taking the seeds of Bletilla bulbocodioides as material, the protocom-like-body development in the aseptic germination of Bletilla seeds and tissue culture and rapid propaation of Bletilla were studied using protocorm and leaves as explants. The results showed that during the process of seed germination,two kinds of globules growth into seedling were discovered:the globules grew into seedlings through their protocorms;the globules grew first into rhizome, then into seedling. The optimum medium for PLB induction was 1/2MS+6-BA 5.0 mg/L+NAA 0.2 mg/L,and the proliferation multiple was 2.71.The tissue culture plantlets were not obtained when using the leaves as explants.
参考文献/References:
[1]扬春澍.药用植物学[M].上海:上海科学技术出版社,1998:326-327. [2]刘光斌,黄忠,黄长于,等.天然植物白芨胶的功能及在化妆品中的应用[J].日用化学品科学,2005,28(8):22-24. [3]张建霞,付志惠,李洪林,等.白芨胚发育与种子萌发的关系[J].亚热带植物科学,2005,34(4):32-35. [4]凃美智,李哖.蝴蝶兰授粉适期与果荚成熟度对种子发芽的影响[J].中国园艺,1987,33(3):190-200. [5]Arditti J，Michaud J D，Healey P L.Morphometry of orchid seeds II.Native California and related species of Calypso，Cephalanthera，Corallorhiza and Epipactis[J].Armer J Bot,1980,67(3):347-360. [6]张燕,黎斌,李思锋.不同培养基上白芨的种子萌发与幼苗形态发生[J].西北植物学报,2009,29(8):1584-1589.
相似文献/References:
[1]郑 晟,邓 赞,张凌云.大白杜鹃种子无菌萌发及幼苗生长条件研究[J].北方园艺,2014,38(11):77.
ZHENG Sheng,DENG Zan,ZHANG Ling-yun.Research on Seeds Aseptic Germination and Seedling Growth Condition of Rhododendron decorum Franch[J].Northern Horticulture,2014,38(03):77.
[2]蹇黎,余丹凤,秦小军.野生白芨叶绿素含量与SPAD值的测定与分析[J].北方园艺,2013,37(21):165.
JIAN Li,YU Dan-feng,QIN Xiao-jun.Comparative Analysis on the Chlorophyll Contents and SPAD Value of Wild Bletilla striata Germplasm Resources[J].Northern Horticulture,2013,37(03):165.
[3]潘 银 萍,李 承 秀,王 长 宪,等.春兰与墨兰杂交种子无菌萌发研究[J].北方园艺,2010,34(23):0.[doi:10.11937/bfyy.201023032]
PAN Yin -ping,LI Cheng -xiu,W ANG Chang -xian,et al.Study of the Pollination Seed about Aseptic Germination of Cymbidium faberi and C. sinense[J].Northern Horticulture,2010,34(03):0.[doi:10.11937/bfyy.201023032]
[4]王宝森,白红丽,张 虹,等.云南蒙自野生白芨中矿质元素含量分析[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102063]
WANG Bao-senI,BAI Hong-li,ZHANG HongI,et al.Analysis on Content of Mineral Elements in Wild Bletilla striata in Mengzi of Yunnan Province[J].Northern Horticulture,2011,35(03):0.[doi:10.11937/bfyy.201102063]
[5]黄春球,宋天顺,李明静,等.白芨花粉活力测定与花粉保存[J].北方园艺,2011,35(03):0.[doi:10.11937/bfyy.201103065]
HUANG ChurrqiuI,SONG TiarrshunI,LI Ming-jingI,et al.Determination of Pollen Vitality and Preserved Method in Bletilla striata (Thunb.)Reichb. f[J].Northern Horticulture,2011,35(03):0.[doi:10.11937/bfyy.201103065]
[6]张瑜,韦廷舟,邹大方,等.白芨SCoT分子标记技术体系的建立及其在快繁苗遗传稳定分析中的应用[J].北方园艺,2018,42(04):152.[doi:10.11937/bfyy.20172477]
ZHANG Yu,WEI Tingzhou,ZOU Dafang,et al.Establishment and Application of SCoT Molecular Marker System for Genetic Stability of Rapid Propagation Plants of Bletilla striata[J].Northern Horticulture,2018,42(03):152.[doi:10.11937/bfyy.20172477]
备注/Memo
第一作者简介：张燕（1979-），女，硕士，助理研究员，现主要从事珍稀濒危植物保护与开发利用等研究工作。E-mail：zhangyan_790603@163.com.