GUO Yan-ru,LIN Jie.Research on the Extraction Technology of Total Flavonoids from Licorice[J].Northern Horticulture,2013,37(24):158-161.
乙醇浸提甘草中总黄酮的工艺研究
- Title:
- Research on the Extraction Technology of Total Flavonoids from Licorice
- 文章编号:
- 1001-0009(2013)24-0158-04
- Keywords:
- licorice; flavonoids; ethanol extraction; response surface
- 分类号:
- S 567.71
- 文献标志码:
- A
- 摘要:
- 以甘草为试材，采用乙醇浸提法提取甘草中黄酮类化合物，通过对提取时间、提取温度、料液比及乙醇浓度进行单因素试验，结合响应面软件Design-Expert 8对4个影响因素进行优化设计，以确定甘草中黄酮类化合物的最佳提取工艺条件。结果表明：响应面优化后的提取条件为乙醇浓度71.02%，料液比1∶32.83 g/mL，提取时间2.97 h，提取温度70.04℃，在该条件下黄酮的最佳得率为1.328%。
- Abstract:
- Taking licorice as material,the best technological conditions of flavonoids compounds from licorice was studied with ethanol extraction method.In the research，the optimal processes for extracting flavonoids compounds from licorice were investigated by four single factors：extraction time，extraction temperature，liquid ratio and concentration of ethanol，combined with the analysis of response surface software Design-Expert 8，which made a optimal design on four influence factors to determine the best technological conditions of flavonoids compounds from licorice.The results showed that the optimum extraction conditions of flavonoids compounds from licorice were the ethanol concentration 71.02%，the solid-liquid ratio 1∶32.83 g/mL，the extraction time 2.97 h，the extraction temperature 70.04℃.Under these conditions，the extraction rate of flavonoids compounds from licorice was 1.328%.
参考文献/References:
[1]赵明春.甘草黄酮的研究进展[C].2010年中国药学大会暨第十届中国药师周大会，2010.
[2]崔永明，余龙江，敖明章，等.甘草总黄酮对油脂抗氧化作用研究[J].基础研究食品科学，2007，28(11):119.
[3]胡小鹰，彭国平，陈汝炎.甘草总黄酮抗心律失常作用研究[J].中草药，1996，27(12)：733-735.
[4]叶怀义，龚赋岚，尚明,等.甘草黄酮抗衰老作用的研究[J].哈尔滨商业大学学报(自然科学版)，2004(2)：20-21.
[5]罗伟强，廖海达，李小梅，等.四种提取柑皮中黄酮物质的方法比较[J].西部粮油科技，2003(1)：50-51.
[6]李炳奇，汪河滨.超声法联合提取甘草黄酮和甘草酸的研究[J].山东中医杂志，2005，24(1)：38-40.
[7]朱菊花.甘草黄酮类化合物提取分离的研究进展[J].中国药业，2010，26(6)：52-53.
[8]尉芹，马希汉.杜仲叶黄酮含量测定方法研究[J].西北农林科技大学(自然科学版)，2001，29(5)：122-199.
相似文献/References:
[1]常桂英,孙立梅,赵 雪,等.长白山刺果甘草与甘草化学成分的比较研究[J].北方园艺,2014,38(10):146.
CHANG Gui-ying,SUN Li-mei,ZHAO Xue,et al.Comparative Study on the Chemical Constituents of Changbai Mountain Glycyrrhiza pallidiflora and Glycyrrhiza uralensis [J].Northern Horticulture,2014,38(24):146.
[2]姜少娟,刘晓莉.生姜黄酮超声提取及其抑菌活性研究[J].北方园艺,2014,38(04):120.
JIANG Shao-juan,LIU Xiao-li.Study on Ultrasonic Extraction of Flavonoids From Ginger and Antibacterial Activity[J].Northern Horticulture,2014,38(24):120.
[3]李红娟,樊金拴,郑涛,等.响应面法优化核桃青皮黄酮的超声提取工艺研究[J].北方园艺,2013,37(14):132.
LI Hong-juan,FAN Jin-shuan,ZHENG Tao,et al.Response Surface Methodology for the Optimization of Extraction Technology of Total Flavonoids from Walnut Green Husk[J].Northern Horticulture,2013,37(24):132.
[4]李 光,余 霜,邓 银,等.金荞麦叶总黄酮含量测定方法研究[J].北方园艺,2013,37(17):154.
LI Guang,YU Shuang,DENG Yin,et al.Study on the Determination Method of Flavonoids in Fagopyrum cymosum Complex Leaves[J].Northern Horticulture,2013,37(24):154.
[5]焦 镭,柴梦颖,石明生,等.碱性条件下山楂叶总黄酮的提取工艺研究[J].北方园艺,2014,38(08):128.
JIAO Lei,CHAI Meng-ying,SHI Ming-sheng,et al.Study on the Extraction Technology of Total Flavonoids From Hawthorn Leaf in Alkaline Conditions[J].Northern Horticulture,2014,38(24):128.
[6]王丽霞,刘坤.超声波辅助提取玉米须黄酮工艺[J].北方园艺,2014,38(06):137.
WANG Li-xia,LIU Kun.Extraction Technology on Flavonoids From Corn Silk by Ultrasonic-assisted[J].Northern Horticulture,2014,38(24):137.
[7]余蕾.微波法提取甘草中类黄酮的工艺研究[J].北方园艺,2014,38(07):130.
YU Lei.Research on Flavonoid Extraction From Glycyrrhiza uralensis Fisch by Microwave Method[J].Northern Horticulture,2014,38(24):130.
[8]王忠忠,马茜,慕金凤,等.宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究[J].北方园艺,2013,37(11):152.
WANG Zhong-zhong,MA Qian,MU Jin-feng,et al.Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.[J].Northern Horticulture,2013,37(24):152.
[9]胡喜巧,陈红芝,孟丽.采收时期对红花苗菜产量及营养成分的影响[J].北方园艺,2013,37(12):165.
HU Xi-qiao,CHEN Hong-zhi,MENG Li.Influence of Harvesting Time on the Yield and Nutritional Composition of Safflowers Vegetable[J].Northern Horticulture,2013,37(24):165.
[10]刘美杰,郝彩琴.宁夏乌拉尔甘草经济施磷量研究[J].北方园艺,2013,37(01):155.
LIU Mei-jie,HAO Cai-qin.Study on Economic Phosphate Levels of Ningxia Ural Licorice[J].Northern Horticulture,2013,37(24):155.
[11]王莹,杜鹏飞,李娟.不同微生物菌肥对甘草光合生理、黄酮含量及土壤微生物的影响[J].北方园艺,2023,(18):103.[doi:10.11937/bfyy.20225277]
WANG Ying,DU Pengfei,LI Juan.Effects of Different Microbial Fertilizers on Photosynthetic Physiology，Flavonoids Content and Soil Microbial Quantity of Glycyrrhiza uralensis[J].Northern Horticulture,2023,(24):103.[doi:10.11937/bfyy.20225277]
备注/Memo
第一作者简介：郭艳茹（1980-），女，内蒙古赤峰人，硕士，讲师，研究方向为生物工程。E-mail:yanruguo2003@126.com.
收稿日期：2013-09-09