[1]李诚,蒋军喜,冷建华,等.猕猴桃枝枯病病原菌鉴定[J].北方园艺,2013,37(24):130-133.
　LI Cheng,JIANG Jun-xi,LENG Jian-hua,et al.Identification of the Pathogen Causing Shoot Blight of Kiwifruit[J].Northern Horticulture,2013,37(24):130-133.
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猕猴桃枝枯病病原菌鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年24期 页码: 130-133 栏目: 出版日期: 2013-12-30
Title:
Identification of the Pathogen Causing Shoot Blight of Kiwifruit
文章编号:
1001-0009(2013)24-0130-04
作者:
李诚1; 蒋军喜1; 冷建华2; 李帮明2; 余强2; 涂贵庆2
1.江西农业大学 农学院，江西 南昌 330045；
2.江西省奉新县农业局，江西 奉新 330700
Author(s):
LI Cheng1; JIANG Jun-xi1; LENG Jian-hua2; LI Bang-ming2; YU Qiang2; TU Gui-qing2
1.College of Agronomy，Jiangxi Agricultural University，Nanchang，Jiangxi 330045；
2.Agricultural Bureau of Fengxin County，Fengxin，Jiangxi 330700
关键词:
猕猴桃枝枯病; 拟茎点霉菌; 隔孢丛壳菌; 葡萄座腔菌
Keywords:
kiwifruit shoot blight; Phomopsis sp.; Glomerella septospora; Botryosphaeria dothidea
分类号:
S 663.4
文献标志码:
A
摘要:
通过病原菌的形态特征、致病性测定和rDNA-ITS序列分析，对引起猕猴桃枝枯病的病原菌进行了分离鉴定。结果表明：猕猴桃枝枯病的主要病原菌为拟茎点霉菌（Phomopsis sp.）、隔孢丛壳菌（Glomerella septospora）和葡萄座腔菌（Botryosphaeria dothidea），3种病菌所占比率分别为39%、38%和23%，且无论是孢子悬浮液接种还是菌丝体接种均只能通过伤口侵染而引起枝条发病。
Abstract:
The pathogen caused shoot blight of kiwifruit were isolated and characterized with the pathogenicity test，morphology and rDNA-ITS sequence assay.The results showed that three kinds of fungi were responsible for shoot blight of kiwifruit in Fengxin County.They were Phomopsis sp.，Glomerella septospora and Botryosphaeria dothidea，which accounted 39%，38% and 23% respectively.Three kinds of fungi penetrated shoot of kiwifruit through wounds including PDA plug inoculation and conidial suspension inoculation.

参考文献/References:

[1]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998.
[2]Murray M G，Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8(19):4321-4325.
[3]王树桐,王亚南,胡同乐,等.板蓝根根腐病病原鉴定[J].植物保护学报,2011,38(5):473-474.
[4]宋晓贺,孙德茂,王明刚,等.陕西石榴干腐病发生及病原菌鉴定[J].植物保护学报,2011,38(1):93-94.
[5]李金花,王蒂,柴兆祥,等.甘肃省马铃薯镰刀菌干腐病优势病原的分离鉴定[J].植物病理学报,2011,41(5)：456-463.
[6]Tsahouridou P C，Thanassoulopoulos C C.Fist report of Alternaria alternata as a dieback pathogen of kiwifruit[J].Plant Disease,2000,84(3):371.
[7]Thomidis T，Exadaktylou E.First report of Botryosphaeria dothidea causing shoot blight of Kiwifruit(Actinidia deliciosa) in Greece[J].Plant Disease,2010,94(12):1053.
[8]Kagawa M，Sato T.Kiwifruit dieback caused by Diaporthe sp.and Botryosphaeria dothidea，kiwifruit soft rot fungi[J].Annals of the Phytopathological Society of Japan,2003,69:373-383.
[9]Rumbos I C，Phillips A J L.A new disease of kiwifruit caused by Botryosphaeria parva[J].South African Journal of Science,2005,101(7/8):314.
[10]Akilli S，Serce C U，Katircioglu Y Z，et al. Involvement of Phytophthora ctiropthora in kiwifruit decline in Turkey[J].Journal of Phytopathology,2011,159:579-581.
[11]Auger J，Perea I，Fullerton R A，et al. First report of verticillium wilt of gold kiwifruit，Actinidia chinensis cv.Hort 16A，caused by Verticillium albo-atrum in Chile[J].Plant Disease,2009,93(5):553.
[12]Polashock J J，Kramer M.Resistance of blueberry cultivars to Botryosphaeria stem blight and Phomopsis Twig blight[J].Hort Scince,2006,41(6):1457-1461.
[13]Ko W H，Koss.A new tiwg blight of mamaki (Pipturus albidus) caused by Botryosphaeria ribis[J].Can J Plant Pathol,2001(23):312-314.
[14]Sobita P H，Bhatt A I，Devi L R，et al.Mosaic disease of broad leaf mustard from North-East India[J].Indian Phytopath,2003,56(2):236-238.
[15]Copes W E，Thomson J L.Survival analysis to determine the length of the incubation period of camellia twig blight caused by Colletotrichum gloeosporioides[J].Plant disease,2008,92:1177-1182.
[16]李诚,蒋军喜,冷建华,等.奉新县猕猴桃果实腐烂病病原菌分离鉴定[J].江西农业大学学报,2012,34(2):259-263.
[17]李诚,蒋军喜,冷建华,等.6种杀菌剂对猕猴桃主要腐烂病菌的室内独立测定[J].中国南方果树,2012,41(1):27-29.
[18]Li C，Jiang J X，Liu L P，et al.Fruit stem blight on kiwifruit (Actinidia chinensis) in China[J].Canadian Journal of Plant Pathology,2013,35(3):411-414.

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备注/Memo

第一作者简介：李诚（1988-），男，硕士研究生，研究方向为分子植物病理学。E-mail:licheng20070242@126.com.
责任作者：蒋军喜（1964-），男，博士，教授，现主要从事植物病害综合治理研究工作。
基金项目：江西省教育厅科学技术研究资助项目（GJJ13269）；科技部国际科技合作资助项目（200DFA31050）。
收稿日期：2013-09-16

更新日期/Last Update: 2014-12-08