WANG Ping,SHEN Xiao-ting,WANG Gang,et al.Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber[J].Northern Horticulture,2013,37(24):84-87.
植物激素对黄瓜愈伤组织形成与芽分化的影响
- Title:
- Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber
- 文章编号:
- 1001-0009(2013)24-0084-04
- Keywords:
- cucumber; phytohormones; cotyledonary nodes; tissue culture
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以黄瓜“津研四号”子叶节为外植体，采用L9（34）正交实验设计，在MS基本培养基中添加6-BA、IAA、ABA、KT 4种植物激素，研究不同激素组合对黄瓜子叶节组织培养芽分化与伸长的影响。结果表明：在MS培养基中添加4种激素中的任何3种均能形成愈伤组织，添加1～2 mg/L 6-BA+0.5 mg/L ABA+4 mg/L KT（或0.050 mg/L IAA）有利于芽分化，添加2 mg/L 6-BA+0.025 mg/L IAA+4 mg/L KT可促进芽的伸长；在培养2周时6-BA、IAA、ABA对再生频率、芽数、芽长的作用值相似，均大于KT的作用；在培养6周时对再生频率、芽数、芽长影响较大的激素是6-BA和ABA；ABA可促进黄瓜芽分化，但在培养6周时抑制芽的伸长。
- Abstract:
- Using cotyledonary nodes of ‘Jinyan 4’ as explants，the orthogonal experiment design L9(34) was applied to investigate the effects of phytohormones combination on differentiation and elongation of buds with MS basic medium plus 4 phytohormones (6-BA,IAA,ABA,KT) in cucumber.The results showed that MS medium plus any three of four phytohormones could form callus，MS medium plus 1~2 mg/L 6-BA，0.5 mg/L ABA and 4 mg/L KT (or 0.050 mg/L IAA) could facilitate the differentiation of adventitious buds obviously in cucumber，whereas，MS medium plus 2 mg/L 6-BA，0.025 mg/L IAA and 4 mg/L KT was beneficial for the elongation of buds.The effects of 6-BA，IAA，ABA on regeneration rates，number of buds and length of buds were similar and more evident than those of KT when the explants were cultured for 2 weeks.Among these 4 phytohormones，6-BA and ABA were demonstrated to be more effective on regeneration rates，number of buds and length of buds relatively when the explants were cultured for 6 weeks.Although ABA could promote of the differentiation of adventitious buds in cucumber，it could suppress the elongation of buds when the explants were cultured for 6 weeks.
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备注/Memo
第一作者简介：王萍（1957-），女，博士，教授，现主要从事植物基因工程与分子生物学研究工作。E-mail:y_pwang@163.com.
基金项目：国家自然科学基金资助项目（31271419）；中央财政支持地方高校发展专项资金资助项目（CXTD07）。
收稿日期：2013-10-24