WANG Wei,XU Jian-min,LI Guang-you,et al.Initial Screening of Insect Resistant Gene to Leptocybe invasa Fisher& La Salle[J].Northern Horticulture,2013,37(24):88-91.
桉树抗枝瘿姬小蜂关联基因的初步筛选研究
- Title:
- Initial Screening of Insect Resistant Gene to Leptocybe invasa Fisher& La Salle
- 文章编号:
- 1001-0009（2013）24-0088-04
- Keywords:
- Leptocybe invasa Fisher&; La Salle; insect resistant gene; transcriptome sequencing; Real-time PCR
- 分类号:
- S 718
- 文献标志码:
- A
- 摘要:
- 以高感品系巨细桉“DH201-2”（样品A）和高抗品系尾叶桉“A107”（样品B）进行转录组测序，测序结果通过Denovo组装、差异表达基因筛选等技术手段筛选出971条差异表达基因，1 224条特异表达基因；对差异基因序列进行BlastP分析和抗性基因功能分析，最终选取了19个目的基因进行实时荧光定量检测。结果表明：19个目的基因中，除了基因G16扩增异常外，其余18个目的基因都获得了较好的特异性扩增，其中，目的基因G1、G5、G9、G10、G18和G19在样品B中的平均表达量显著高于其在样品A中的平均表达量，目的基因G3、G4和G11在样品A中的平均表达量显著高于其在样品B中的表达量；其余目的基因在2个样品中表达量差异不显著。目的基因G1、G3、G4、G5、G9、G10、G11、G18和G19可能是与桉树抗枝瘿姬小蜂有关联的基因。
- Abstract:
- The high-sensitive strain of Eucalyptus ‘DH201-2’ (sample A) and high-resistant strain of Eucalyptus urophylla ‘A107’ (sample B) were chosen for transcriptome sequencing,971 differential expression sequences were screened through de novo assembly and differential expression analysis for sequence.19 target genes were chosen to make RT-PCR.The results showed that the target gene had a good specificity amplification except the target gene 16.The average amount of expression of G1,G5,G9,G10,G18 and G19 in sample B was significant higher than in sample A.The average amount of expression of G3,G4 and G11 in sample A was significantly higher than in sample B.There were no significant differences of the average amount of expression among the last target gene.Target gene G1,G3,G4,G5,G9,G10,G11,G18,G19 maybe the susceptibility gene to insect resistant gene to Leptocybe invasa Fisher& La Salle.
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相似文献/References:
[1]陈元生,廖忠明,涂小云.赣南桉树品种(系）对桉树枝瘿姬小蜂的抗性研究[J].北方园艺,2015,39(11):106.[doi:10.11937/bfyy.201511026]
CHEN Yuan-sheng,LIAO Zhong-ming,TU Xiao-yun.Study on Resistance of Different Eucalyptus Varieties to Leptocybe invasa in Southern Jiangxi[J].Northern Horticulture,2015,39(24):106.[doi:10.11937/bfyy.201511026]
备注/Memo
第一作者简介：王伟（1983-），男，山西临汾人，博士研究生，研究方向为观赏植物栽培及育种。E-mail:waynelove@126.com.
责任作者：徐建民（1964-），男，博士，研究员，博士生导师，现主要从事林木遗传育种等研究工作。E-mail：jianmxu@163.com.
基金项目：国家“十二五”科技支撑计划资助项目（2012BAD01B0401）；中央级公益性科研院所基本科研业务费专项资金资助项目（RITFYWZX200904）；广州市林业和园林局科研资助项目。
收稿日期：2013-09-13