PEI Dong-li,WANG Chong,LI Cheng-wei.Study on POD Enzyme Activity and Isozyme Pattern Analysis of Peppers Under Low Phosphorous Stress[J].Northern Horticulture,2013,37(23):113-116.
低磷胁迫下辣椒幼苗过氧化物酶活性及其同工酶谱的分析
- Title:
- Study on POD Enzyme Activity and Isozyme Pattern Analysis of Peppers Under Low Phosphorous Stress
- 文章编号:
- 1001-0009(2013)23-0113-04
- 关键词:
- 辣椒; 低磷胁迫; 过氧化物酶（POD）; 同工酶
- Keywords:
- peppers; low phosphorous stress; peroxidase(POD); isozyme
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 以4个辣椒品种“牛角椒”、“南纬金峰二号”、“茄门椒”和“8819线椒”为试材，采用水培方法，研究了常磷（1 mmol/L）和低磷（0.01 mmol/L）2个磷水平处理对不同辣椒品种叶片中过氧化物酶（POD）活性及其同工酶酶谱的影响。结果表明：低磷胁迫下，“牛角椒”、“茄门椒”和“8819线椒”的POD活性随着胁迫时间的延长出现先升高再下降随后又升高的趋势，“南纬金峰二号”的POD活性在整个胁迫过程一直升高；过氧化物同工酶谱的变化与酶活性变化相一致，低磷组酶活性均显著高于同期常磷组，且酶谱染色深度和谱带条数均高于同期常磷组，说明POD活性与植物的耐低磷性有关。
- Abstract:
- Taking four varieties of peppers leaves of ‘Niujiao pepper’，‘Qiemen pepper’，‘Nanweijinfeng 2’ and ‘8819 line pepper’ as test materials, with water culture，the effect of 1 mmol/L and 0.01 mmol/L phosphorus (P) on peroxidase (POD) activity and their isoenzymic bands were studied.The results showed that POD activity in leaves of ‘Niujiao pepper’，‘Qiemen pepper’ and ‘8819 line pepper’ increased firstly，decreased secondly and then increased again with time，while the POD activity of ‘Nanweijinfeng 2’ increased during the whole test time，and the changing trend of POD isozyme zymogram was accordance with POD activity.The enzymatic activity under low phosphorus was stronger than that of normal phosphorus treatment and the isozyme bands and expression quantity of POD were more than that of normal phosphorus treatment at the same time.The result suggested that POD was related with plant against low phosphorous press.
参考文献/References:
[1] 易籽林,赵坤,董文武,等.辣椒高温胁迫研究进展[J].辣椒杂志,2011(3):5-9.
[2] 鲁如坤.中国土壤氮、磷、钾的基本状况[J].土壤学报,1989,26(3):280-286.
[3] 章爱群,贺立源,李德华.作物耐低磷营养性状遗传研究进展[J].山地农业生物学报,2008,27(2):162-169.
[4] 彭永康,张丰德.不同剂量Co-r射线对小麦水稻幼苗生长的影响[J].华北农业学报,1987,2(1):13-18.
[5] 孙静,王宪泽.盐胁迫对小麦过氧化物酶同工酶基因表达的影响[J].麦类作物学报,2006,26(11):42-44.
[6] 郑金凤,董少鸣,李成璞,等.低磷胁迫对小麦代换系保护酶活性和丙二醛含量的影响及染色体效应[J].植物营养与肥料学报,2010,16(6):1366-1372.
[7] 王晶,韩晓日,站秀梅,等.低磷胁迫对番茄叶片膜脂过氧化及保护酶活性的影响[J].植物营养与肥料学报,2005,11(6):851-854.
[8] 李志刚,宋书宏,李瑞平,等.磷胁迫下大豆叶片及根部超氧化物歧化酶酶谱的研究[J].内蒙古民族大学学报,2008,23(2):147-149.
[9] 张宪政,陈风玉,王荣富.植物生理学试验技术[M].沈阳:辽宁科学技术出版社,1994:45-48.
[10]张志良.植物生理学实验指导[M].1版.北京:高等教育出版社,1990:367-219.
[11]冯佰利,高小丽,王长发,等.干旱条件下不同温型小麦叶片衰老与活性氧代谢特性的研究[J].中国生态农业学报,2005,13(4):74-76.
[12]邵雪梅,范金梅.树轮宽资料所指示的川西过去气候变化[J].第四纪研究,1999(1):81-89.
[13]敖雪.磷素对不同磷效率基因型大豆的影响[D].沈阳:沈阳农业大学,2009.
[14]谭晓荣,吴兴泉,杜书文,等.干旱胁迫对小麦幼苗活性氧清除能力的影响[J].安徽农业科学,2006,34(15):3565-3567.
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(23):6.
[3]张婷玉,林 多,杨延杰.辣椒根系分泌物的收集方法研究[J].北方园艺,2014,38(12):14.
ZHANG Ting-yu,LIN Duo,YANG Yan-jie.Study on Collection Method of Root Exudates on Pepper[J].Northern Horticulture,2014,38(23):14.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(23):50.
[5]孔小平,苗增建.等离子体处理辣椒种子对其性状及产量的影响[J].北方园艺,2013,37(13):34.
KONG Xiao-ping,MIAO Zeng-jian.Effect of Plasma Treatment on Characters and Output of Pepper Seeds[J].Northern Horticulture,2013,37(23):34.
[6]高万里.白城地区辣椒产业发展对策研究[J].北方园艺,2014,38(04):159.
GAO Wan-li.Study on the Development Countermeasure of Pepper Industry in Baicheng Area[J].Northern Horticulture,2014,38(23):159.
[7]岳振平.不同种植密度对早春大棚辣椒产量和产值的影响[J].北方园艺,2014,38(05):51.
YUE Zhen-ping.Effect of Different Planting Densty on Fruit Yield and Output Value of Pepper in Early Spring[J].Northern Horticulture,2014,38(23):51.
[8]郭亚华,谢立波,王雪,等.辣椒经地面模拟诱变后的RAPD鉴定[J].北方园艺,2014,38(05):95.
GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(23):95.
[9]杨飞,高艳明,李建设.宁夏设施沙培辣椒引种试验[J].北方园艺,2013,37(10):44.
YANG Fei,GAO Yan-ming,LI Jian-she.Introduction Test of Sand Culture Facilities Pepper in Ningxia[J].Northern Horticulture,2013,37(23):44.
[10]马寿宾,孙艳,王琛,等.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013,37(10):107.
MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(23):107.
备注/Memo
第一作者简介：裴冬丽（1971-），女，博士，教授，研究方向为植物病原菌互作分子生物学。E-mail:peidongli@126.com.
基金项目：国家自然科学基金资助项目（31071807）；河南省教育厅资助项目（13B210199）。
收稿日期：2013-07-24