GAO Xiu-yan,ZHAO Yu-hui,GUO Yin-shan,et al.Methodology Investigation of Reversal HPLC Measuring the Content of Fruit Polyphenol in Hawthorn[J].Northern Horticulture,2013,37(23):106-108.
利用反相高效液相色谱法检测山楂果实多酚含量的方法学研究
- Title:
- Methodology Investigation of Reversal HPLC Measuring the Content of Fruit Polyphenol in Hawthorn
- 文章编号:
- 1001-0009（2013）23-0106-03
- Keywords:
- hawthorn; fruit polyphenol; HPLC
- 分类号:
- S 661.5
- 文献标志码:
- A
- 摘要:
- 以山楂为试材，采用反相高效液相色谱技术，建立同时检测山楂果实中多种多酚含量的方法。结果表明：高效检测体系：色谱柱Agilent ZORBAX Extend-C18柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.5%磷酸水溶液，梯度洗脱：0～9 min（18∶82～20∶80），9～25 min（20∶80～50∶50），25～33 min（50∶50～100∶0）；检测波长280 nm；柱温30℃，流速0.9 mL/min，进样量20 μL，该试验可在同一色谱条件下，同时测定山楂果实中黄酮类和原花青素类的含量，通过精密度、重复性、稳定性和加样回收率试验，证明课题组前期建立的供试品溶液的制备方法，也适用于原花青素类的提取。
- Abstract:
- With hawthorn as material,by reverse phase high performance liquid chromatography (HPLC)，method of detection of various polyphenol content in hawthorn fruit were establised.The results showed that the high performance detection system was as follows：chromatographic column was Agilent ZORBAX Extend-C18 colume (250 mm×4.6 mm，5 μm)；moving phase was acetonitrile-0.5% phosphoric acid solution；gradient elution：0～9 min（18∶82～20∶80），9～25 min（20∶80～50∶50），25～33 min（50∶50～100∶0）；testing wavelength was 280 nm；column temperature was 30℃；flow velocity was 09 mL/min，injection volumn was 20 μL.This method can determine flavone and procyanidine contents in hawthorn fruit simultaneously under the same condition.According to the accuracy test，repeatability test，stability test it and recovery test indicated that the previously established sample solution preparation method was also suitable for procyanidine abstraction.
参考文献/References:
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备注/Memo
第一作者简介：高秀岩（1963-），女，本科，副教授，研究方向为果树种质资源评价与利用。E-mail：gaoxy024@163.com.
基金项目：国家自然科学基金资助项目（311101515）；沈阳农业大学青年教师基金资助项目（20101017）。
收稿日期：2013-07-25