WANG Shang-de,WANG Xiao-chuan,LIU Jia-shen,et al.Study on Nectarine Triploidy Germplasm Propogation by Hardwood Cutting[J].Northern Horticulture,2013,37(23):8-11.
油桃三倍体种质的硬枝扦插技术研究
- Title:
- Study on Nectarine Triploidy Germplasm Propogation by Hardwood Cutting
- 文章编号:
- 1001-0009(2013)23-0008-04
- Keywords:
- nectarine; triploid; hardwood cutting
- 分类号:
- S 662.1
- 文献标志码:
- A
- 摘要:
- 以油桃三倍体种质‘05-22-49’为试材,通过正交实验筛选影响扦插的关键因素;通过双因子完全组合设计,筛选生长调节剂种类、浓度和处理方式的最佳组合,以期建立油桃三倍体硬枝扦插技术体系。结果表明:对生根率和生根量影响最大的是扦插环境;对移栽成活率影响最大的是插穗的基部流水冲洗;综合考虑生根率、生根量和移栽成活率,最优的三倍体扦插生根方式为:基部剪口冲洗24 h+IBA 1 000 mg/L+热激处理6 d+日光温室培养,扦插成活率达82%;低浓度的IBA浸泡处理效果不及高浓度速蘸处理有效;ABT生根粉(1号)处理插穗与IBA处理的生根率持平、生根量略低。
- Abstract:
- Taking nectarine triploidy germplasm ‘05-22-49’ as material,key factors were selected by orthogonal test,types and content of auxins were decided by double factor test,in order to establish the propagation method of triploidy nectarine from hardwood cuttings.The results showed that culture condition was the most important factor for rhizogenesis,and water rushing was more important for survival rate of transplanting.Optimized integrative protocol for triploidy nectarine propagation form hardwood cutting was as follows:cutting basal tissues be washed for 24 h in running water,then be treated as follows:diped in 1 000 mg/L IBA solution,inserted into mix medium (perlite∶peat=3∶1),maintain a temperature of 19℃ around the basal portion of the cuttings and upper part remains exposed to the cooler ambient temperature for 6 days,cultured in solar greenhouse without heating facilities.By the protocol,cuttings survival rate could up to 82%.Immersing cutting basal tissues in low content IBA solution was not better than dipping.ABT(1) rooting powder was an alternative type auxin of IBA for their similar rooting rate.
参考文献/References:
[1] Pratassenjia G D.Production of polyploidy plants.Haploids and triploids in Prunus persica[J].Comprend Acad Sci(Doklady) URSS,1939,22:348-351.?
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备注/Memo
第一作者简介:王尚德(1976-),男,硕士,副研究员,现主要从事果树种质资源收集及评价和创新等研究工作。E-mail:shangdee@163.com.