HUJin-ge,LIAOXin-fu,LUOShu-ping,et al.GeneticDiversityAnalysisofMelonGermplasmResourcesResistantto StoragePropertiesbyRAPDMolecularMarkers[J].Northern Horticulture,2013,37(16):120-124.
甜瓜种质资源耐贮性状的RAPD分子标记筛选
- Title:
- GeneticDiversityAnalysisofMelonGermplasmResourcesResistantto StoragePropertiesbyRAPDMolecularMarkers
- 文章编号:
- 1001-0009(2013)16-0120-05
- Keywords:
- melon; RAPD; germplasmresources
- 分类号:
- S652
- 文献标志码:
- A
- 摘要:
- 以64份不同耐贮性状的甜瓜种质资源为试材,采用RAPD分子标记技术对其进行了遗传多样性分析。结果表明:19条引物共扩增出118条DNA条带,其中103条表现出多态性,占总条带的87.29%。聚类分析结果表明,64份甜瓜种质资源可被分为耐贮藏、不耐贮藏和中间类型三大类。
- Abstract:
- 64differentmelonvarietiesresistanttostoragepropertieswereusedtoanalyzegeneticdiversitybyRAPDmarker.Theresultsshowedthat118DNAbandswereamplifiedwith19randomprimers,ofwhich103werepolymorphic.Anaverageproportionofpolymorphiclociwas87.29%.64melonvarietiescouldbeclassifiedintothreegroups,whichwerestorable,intolerancetostorageandmediumstorabletype.
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备注/Memo
第一作者简介:户金鸽(1982-),女,硕士,助理研究员,现主要从事西甜瓜育种等研究工作。E-mail:hujinge2007@sina.com.
责任作者:廖新福(1960-),男,硕士,研究员,硕士生导师,国家西甜瓜产业技术体系岗位专家,现主要从事西甜瓜育种和贮藏与运输等研究工作。E-mail:lxf3838@163.com.
基金项目:国家西甜瓜产业技术体系专项资金资助项目(nycytx-36);新疆自治区科技支撑资助项目(201131104)。
收稿日期:2013-04-10