[1]段亚楠,卢福荣,茹艺,等.紫花苜蓿组织培养高频再生体系的建立[J].北方园艺,2013,37(15):127-129.
　DUAN Ya-nan,LU Fu-rong,RU Yi,et al.Establishment of High Frequency Regeneration System of Medicago sativa[J].Northern Horticulture,2013,37(15):127-129.
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紫花苜蓿组织培养高频再生体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年15期 页码: 127-129 栏目: 出版日期: 2013-07-15
Title:
Establishment of High Frequency Regeneration System of  Medicago sativa
文章编号:
1001-0009(2013)15-0127-03
作者:
段亚楠; 卢福荣; 茹艺; 李曹娜; 韩阳
辽宁大学 生命科学院，辽宁 沈阳 110036
Author(s):
DUAN Ya-nan; LU Fu-rong; RU Yi; LI Cao-na; HAN Yang
School of Life Sciences,Liaoning University,Shenyang,Liaoning 110036
关键词:
紫花苜蓿; 子叶; 组织培养; 植株再生
Keywords:
alfalfa; cotyledon; plant tissue culture; plant regeneration
分类号:
S 541+1
文献标志码:
A
摘要:
以紫花苜蓿品种“甘农1号”的子叶为外植体，研究了不同培养基和不同浓度激素对紫花苜蓿愈伤组织诱导、分化及生根的影响。结果表明：最佳愈伤组织诱导培养基为NSH+2,4二氯苯氧基（2,4-D）4 mg/L+6苄氨基嘌呤（6-BA）0.4 mg/L+3%蔗糖+0.7%琼脂；最佳诱导愈伤组织分化培养基为MS+2%蔗糖+0.7%琼脂；最佳诱导无根苗生根培养基为1/2MS+萘乙酸（NAA）0.2 mg/L+1%蔗糖+0.7%琼脂；再生植株适宜的移栽基质为营养土∶草炭∶蛭石=1∶2∶1。该研究建立了一个紫花苜蓿稳定的高频再生体系，为其作为转基因受体奠定了基础。
Abstract:
Taking the cotyledons of  Medicago sativa‘Gannong No.l’ as explants,the effects of different induction media and different concentrations of hormone on influence of callus induction,differentiation and rooting were investigated.The results showed that NSH+2,4D 4 mg/L+6-BA 0.4 mg/L+3% sucrose+0.7% agar was the optimal medium for callus induction；MS+2% sucrose+0.7% agar was the optimal media for differentiation；MS+NAA 0.2 mg/L+1% sucrose+0.7% agar was the optimal medium for rooting.The regenerative plant could survive after transplanting into the suitable substrate consisted of nutritive soil∶peat∶vermiculite＝1∶2∶1.This study built an stable high frequency regeneration system for alfalfa,and laid a foundation for the transgenic receptor.

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备注/Memo

第一作者简介：段亚楠（1987-），女，山东聊城人，硕士研究生，研究方向为植物分子生物学。Email:dyn87219@126.com.
责任作者：韩阳（1962-），女，博士，教授，硕士生导师，研究方向植物生物技术。E-mail:hanyang_0802@163.com.
基金项目：辽宁省教育厅重点实验室资助项目（LS2010070）；辽宁省自然科学基金资助项目（201202083）。
收稿日期：2013-04-08

更新日期/Last Update: 2014-08-12