[1]颜秦峰,李科友,石振楠,等.过表达miR398对烟草抗旱性的影响[J].北方园艺,2013,37(13):111-116.
　YAN Qin-feng,LI Ke-you,SHI Zhen-nan,et al.Effect of Over expressed miR398 on the Drought Resistance of Nicotiana tabacum[J].Northern Horticulture,2013,37(13):111-116.
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过表达miR398对烟草抗旱性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年13期 页码: 111-116 栏目: 出版日期: 2013-07-15
Title:
Effect of Over expressed miR398 on the Drought Resistance of Nicotiana tabacum
文章编号:
1001-0009(2013)13-0111-06
作者:
颜秦峰; 李科友; 石振楠; 王琳; 武永军
西北农林科技大学 生命科学学院，陕西 杨凌 712100
Author(s):
YAN Qin-feng; LI Ke-you; SHI Zhen-nan; WANG Lin; WU Yong-jun
College of Life Sciences，Northwest Agricultural and Forestry University，Yangling，Shaanxi 712100
关键词:
miR398; 转基因; 烟草; 抗旱性
Keywords:
MiR398; transgenic; Nicotiana tabacum; drought resistance
分类号:
S 572
文献标志码:
A
摘要:
以烟草为试材，构建了拟南芥MIR398过表达载体，对烟草进行了转基因试验，研究了转基因烟草T1代与野生型烟草在干旱条件下超氧阴离子、丙二醛、游离脯氨酸含量的差异。结果表明：该试验成功将拟南芥MIR398基因转入烟草中，与野生型相比，转基因烟草在干旱胁迫下丙二醛与超氧阴离子含量都有所升高，脯氨酸含量则相对降低。该试验结果说明了miR398在植物抗旱过程中的作用。
Abstract:
Taking Nicotiana tabacum as material，an over expressing MIR398 plasmid vector was constructed and introduced into the Nicotiana tabacum successfully.Some physiological indicators such as superoxide anion radical，malondialdehyde (MDA) and proline (Pro) content was studied and compared between wild type tabacum and transgenic plant T1 generation.The results showed that MDA and superoxide anion radical content accumulated more than wildtype plant，while Pro content was declined under the drought stress in the transgenic plant.This result further proved the role of miR398 in plant drought resistance.

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备注/Memo

第一作者简介：颜秦峰（1989-），男，硕士，现主要从事植物抗逆分子生物学等研究工作。E-mail：namelessfit@hotmail.com.

责任作者：武永军（1974-），男，博士，副教授，现主要从事植物抗逆分子生物学等研究工作。E-mail：wuyongjun@nwsuaf.edu.cn.
基金项目：高校基本科研业务费专项资助项目；国家自然科学基金资助项目（30600384）；西北农林科技大学大学生创新性实验计划资助项目。
收稿日期：2013-03-19

更新日期/Last Update: 2014-08-08