GUO Peng-wei,GAO Ye-hua,GAO Ri,et al.Study on Proliferation of Virusfree Strawberry ‘Bechika’[J].Northern Horticulture,2013,37(10):110-113.
“贝吉佳”草莓脱毒苗增殖的研究
- Title:
- Study on Proliferation of Virusfree Strawberry ‘Bechika’
- 文章编号:
- 1001-0009(2013)10-0110-04
- Keywords:
- virusfree seeding; proliferation; culture medium; hormone; sugar source
- 分类号:
- S 668.4
- 文献标志码:
- A
- 摘要:
- 以“贝吉佳”草莓脱毒苗为外植体，研究了培养基种类、激素种类及浓度、糖源种类及浓度、光照强度等对组培苗增殖的影响。结果表明：在MS培养基、BA浓度为2.0 mg/L、糖源为30 g/L的蔗糖、光照强度在1600 lx条件下附加0.1 mg/L NAA的培养基最适合脱毒苗的增殖，增殖系数可达12.0。
- Abstract:
- Taking virus free seeding of strawberry ‘Bechika’ as explants，the effects of culture media，hormone types and concentrations，sugar types and concentrations，illumination intensities on tissue culture proliferation were studied.The results showed that the MS basic culture medium，with BA 2.0 mg/L，sugar 30g/L as carbon source，the illumination intensity at 1600 lx，additional with NAA 0.1 mg/L were most suitable for the proliferation of virusfree seeding，and the proliferation index could be up to 12.0.
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备注/Memo
第一作者简介：郭朋伟(1988-)，男，硕士研究生，研究方向为生物工程及栽培生理。
责任作者：吴荣哲(1966-)，男，博士，副教授，现主要从事生物工程及栽培生理等研究工作。
收稿日期：2013-01-18