YANG Rui,ZHAO Bao-long,ZHANG Li,et al.Biological Characteristics of the Pathogen of Kuerle Pear Black Spot and Screen of Fungicides in the Laboratory[J].Northern Horticulture,2013,37(05):109-113.
库尔勒香梨黑斑病菌生物学特性及其室内药剂筛选
- Title:
- Biological Characteristics of the Pathogen of Kuerle Pear Black Spot and Screen of Fungicides in the Laboratory
- 文章编号:
- 1001-0009(2013)05-0109-05
- 分类号:
- S 436.612.1+4
- 文献标志码:
- A
- 摘要:
- 为了进一步了解库尔勒香梨黑斑病菌生物学特性，通过接种试验,研究了不同外界条件因子对病原菌菌丝生长和分生孢子萌发的影响。结果表明：病原菌菌丝最适生长温度为25~28℃，最适pH为6~7;孢子萌发最适温度为28~30℃，最适pH为7。分生孢子在梨果肉和表皮上萌发率差异不显著，且在梨表皮30℃培养12 h，萌发率可达95.8%。同时证明接种病菌发酵液粗体毒素可导致库尔勒香梨发病。对于7种杀菌剂室内抑制病原菌分生孢子萌发试验，得出甲基托布津对分生孢子萌发的抑制效果最好，其2 000倍液抑菌率为92%。
- Abstract:
- In order to further understand biological characteristics of the pathogen caused Kuerle pear black spot in Xinjiang，the pathogenicity of the strain LH08 by needling methods and effection different environment factors on mycelia growth and conidium germination were studied.The results showed that the optimum temperature for mycelia growth and spore germination were 25~30℃,28~30℃，respectively.The optimum pH value for mycelia growth was 6，and the optimum pH value for spores germination was 7.There was not significant difference in the rate of conidium germination between on the pear pulp and on the pear pericarp.The germination rate could reach 95.8% at 30℃ for 12 h on the pear pericarp.In addition，the symptom induced by artificial inoculation with crude extract toxins produced by the strain LH08 was like the symptoms in the field，too.Further，it implied that the toxins might play an important role in infection.The results of chemical screening among seven kinds of fungicide showed that thiophanate methyl 70% WP was most effective to inhibition conidium germination.The inhibition rate was 92% at 2 000 times diluent of thiophanate methyl.
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备注/Memo
第一作者简介：杨瑞（1982-），男，本科，现主要从事植物病害及其防治研究工作。E-mail：wxdong11@163.com.
责任作者：王晓东（1975-），男，新疆石河子人，博士，副教授，现主要从事植物病害生物防治研究工作。E-mail:wxd_agr@shzu.edu.cn.
基金项目：石河子大学高层次人才科研启动资助项目（RCZX200906）。
收稿日期：2012-11-09