GONG Na,YANG Zhen,WANG Na,et al.Screening on the Seed Medium of Mycorrhizal Fungi of Blueberry[J].Northern Horticulture,2012,36(20):110-112.
越橘菌根菌种子培养基的筛选
- Title:
- Screening on the Seed Medium of Mycorrhizal Fungi of Blueberry
- 文章编号:
- 1001-0009(2012)20-0110-03
- Keywords:
- mycorrhizal fungi; medium; fermentation
- 分类号:
- Q 948.9
- 文献标志码:
- A
- 摘要:
- 以菌丝长速和菌丝干质量为测量指标,结合液体发酵培养生长曲线,研究了越橘菌根菌菌丝生长的最佳培养基和最佳培养时间。结果表明:从4种培养基中初筛出PDMA培养基为适合该菌株生长的基础培养基,得到了该菌株种子培养的最佳液体转接时间为液态发酵的第2~3天,最佳生产时间为第5天,产量最高,为3.45 g/100mL。
- Abstract:
- Taking growth rate and dry weight of mycelium as measurement index,combined with the growth curve of liquid fermentation culture,PDMA medium was found suitable as basic medium for this strain after 4 kinds of medium were preliminarily screened.And the optimal liquid transfer time for culturing the seed of this strain was the 2~3 d of liquid fermentation;the best production time was the 5 d,where the yield was the highest as 3.45 g/100mL.
参考文献/References:
[1]於虹.蓝浆果栽培与采后处理技术[M].北京:金盾出版社,2003. [2]Stribley D P,Read D J.The biology of mycorrhiza in the Ericaceae.The relationship between mycorrhizal infection and the capacity to utilize simple and complex organic nitrogen source[J].New Phytol,1980,86:365-371. [3]李敏.褐环乳牛肝菌(Suillus luteus (L.:Fr)Gray)发酵生物学的研究[D].呼和浩特:内蒙古农业大学,2007. [4]段慧.平菇汁对褐环粘盖牛肝菌生长的影响[J].内蒙古农业大学学报,2009,30(4):310. [5]杨海龙,活泼,肖彩霞,等.药用真菌深层发酵生产技术[M].北京:化学工业出版社,2009. [6]乐超银,邵伟.猴头菌液体发酵条件的研究[J].中国食用菌,1999(3):32-34. [7]蔺银鼎,马宏艳.竹荪液体增培养基础优化配方筛选及培养基营养变化特性的研究[J].食用菌学报,2007(4):18-24.
相似文献/References:
[1]李丽丽,杨洪一.杜鹃花类菌根真菌研究进展[J].北方园艺,2014,38(11):173.
LI Li-li,YANG Hong-yi.Research Advances of Ericoid Mycorrhizal Fungi[J].Northern Horticulture,2014,38(20):173.
备注/Memo
第一作者简介:龚娜(1982-),女,硕士,助理研究员,现主要从事微生物工程及生物技术等研究工作。E-mail:doll52133@163.com.