WANG Juan.Nature of Peroxidase in Feicheng Peach Fruit[J].Northern Horticulture,2012,36(15):28-30.
肥城桃果实中过氧化物酶的特性研究
- Title:
- Nature of Peroxidase in Feicheng Peach Fruit
- 文章编号:
- 1001-0009(2012)15-0028-03
- Keywords:
- Feicheng peach; peroxidase; kinetic characteristics; isoenzyme
- 分类号:
- TS 255.3
- 文献标志码:
- A
- 摘要:
- 以肥城桃果实为试材，分离纯化了引起酶促褐变的过氧化物酶（POD），并对其性质进行了分析。结果表明：POD仅存在于可溶性组织中，以绿原酸和过氧化氢（H2O2）为底物POD的Km值分别为0.36和0.48 mmol/L，以4-甲氧基-α-萘酚（4MN）和H2O2为底物POD的Km值分别为0.39和0.17 mmol/L。以绿原酸为底物时POD对H2O2的催化效率（Vm/Km）显著高于以4MN为底物时的催化效率。用等电聚焦和阳离子非变性聚丙烯酰胺凝胶电泳分析POD，均检测到2条碱性POD同工酶条带，用Western分析不能区分POD的2种同工酶。
- Abstract:
- The peroxidase (POD) involved in enzymatic browning reactions was extracted from Feicheng peach fruit and its characteristics were analyzed.The results showed that POD was found only in the soluble tissues.The Km value were 0.36 mmol/L and 0.48 mmol/L respectively with the substrate of chlorogenic acid and H2O2.The Km value were 0.39 mmol/L and 0.17mmol/L respectively with the substrate of 4-methoxyl-α-naphthol and H2O2.The catalytic efficiency (Vm/Km) of POD on H2O2 with the substrate of chlorogenic acid was much higher than that with the substrate of 4-methoxyl-α-naphthol.The characteristics of POD were also analyzed with isoelectric focusing and cationic native polyacrylamide gel electrophoresis.Two POD alkaline isoenzymes were detected and they were not distinguished with western blotting.
参考文献/References:
[1]吴振先,苏美霞,陈维信,等.贮藏荔枝果皮多酚氧化酶及过氧化物酶与褐变的研究[J].华南农业大学学报,1998,19(1):12-15. [2]李富军,翟衡,杨洪强,等.1-MCP和AVG对肥城桃果实采后衰老的影响[J].果树学报,2004,21(3):272-274. [3]Zhu S H，Liu M C，Zhou J.Inhibition by nitric oxide of ethylene biosynthesis and lipoxygenase activity in peach fruit during storage[J].Postharvest Biology and Technology,2006,42:41-48. [4]朱树华,刘孟臣,周杰.一氧化氮熏蒸对采后肥城桃果实细胞壁代谢的影响[J].中国农业科学,2006,39(9):1878-1884. [5]Bordier C.Phase separation of integral membrane proteins in Triton X-114 solution[J].Journal of Biological Chemistry,1981,256(4):1604-1607. [6]Ferrer M A，Calderon A A，Munoz R，et al.4-Methoxy-α-naphthol as a specific substrate for kinetic，zymographic and cytochemical studies on plant peroxidase activities[J].Phytochemical Analysis,1990(1)：63-69. [7]Reisfeld R A，Lewis U J，Williams D E.Disk electrophoresis of basic proteins and peptides on polyacrylamide gels [J].Nature,1962,195(4838):281-283. [8]O’farrell P H.High resolution two-dimensional electrophoresis of proteins[J].Journal of Biological Chemistry，1975,250(10):4007-4021. [9]Escribano J，Gandia-herrero F，Caballero N，et al.Subcellular localization and isoenzyme pattern of peroxidase and polyphenol oxidase in beet root (Beta vulgaris?L.)[J].Journal of Agricultural and Food Chemistry,2002,50(21):6123-6129. [10]Gandia-herrero F，Jimenez M，Cabanes J，et al.Tyrosinase inhibitory activity of cucumber compounds：Enzymes responsible for browning in cucumber[J].Journal of Agricultural and Food Chemistry,2003,51(26)：7764-7769. [11]Hoyle M C.High resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing [J].Plant Physiology,1977,60(5):787-793. [12]Lee H C，Klein B P.A simple method of identifying peroxidase isoenzymes from crude pea seed extracts [J].Food Chemistry,1988,29(4):275-282. [13]Mclellan K M，Robinson D S.The heat stability of purified spring cabbage peroxidase isoenzymes[J].Food Chemistry,1987,26(2):97-107. [14]Flurkey W H.Identification of tyrosinase in mushrooms by isoelectric focusing[J].Journal of Food Science，1991,56(1):93-95. [15]Gabaldon C，Lopez-serrano M，Pedreno M A，et al.Cloning and molecular characterization of the basic peroxidase isoenzyme from Zinnia elegans，an enzyme involved in lignin biosynthesis [J].Plant Physiology，2005,139(3):1138-1154. [16]Barcelo A.R，Gomezros L V，Gabaldon C，et al.Basic peroxidases：The gateway for lignin evolution[J].Phytochemistry Reviews,2004,3(1):61-78. [17]Lopez-serrano M，Fernandez M D，Pomar F，et al.Zinnia elegans uses the same peroxidase isoenzyme complement for cell wall lignification in both single-cell tracheary elements and xylem vessels[J].Journal of Experimental Botany,2004,55(396):423-431.
相似文献/References:
[1]张华,董月强,李星科,等.酸性氧化电位水对鲜切莲藕保鲜的效果[J].北方园艺,2013,37(13):163.
ZHANG Hua,DONG Yue-qiang,LI Xing-ke,et al.Effect of Acid Oxidation potential Water on Preservation of Fresh cut Lotus Root[J].Northern Horticulture,2013,37(15):163.
[2]张书玲,刘建凤,尚爱芹.银条根茎膨大过程中生理生化变化的研究[J].北方园艺,2012,36(03):168.
ZHANG Shu-ling,LIU Jian-feng,SHANG Ai-qin.Study on the Change of Physiological-biochemical Changes ofManyflower Betony During Rhizome Intumescence[J].Northern Horticulture,2012,36(15):168.
[3]孙睿,邵红,刘娟,等.北马兜铃营养生长期过氧化物酶同工酶分析[J].北方园艺,2012,36(16):88.
SUN Rui,SHAO Hong,LIU Juan,et al.Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase[J].Northern Horticulture,2012,36(15):88.
[4]袁继杰,张志.六个矮牵牛品种生理指标比较分析[J].北方园艺,2012,36(06):67.
YUAN Ji-jie,ZHANG Zhi.Comparative Analysis of Physiological Indices to Six Petunia hybrida Varieties[J].Northern Horticulture,2012,36(15):67.
[5]莫帼超,谢国斌,陈奇,等.青海东南部地区不同居群山丹百合POD同工酶分析[J].北方园艺,2013,37(16):108.
MOGuo-chao,XIEGuo-bin,CHENQi,et al.IsozymesofPeroxideasaAnanlysisBetweenPopulationsof Liliumpumilum DC.inSoutheastQinghai[J].Northern Horticulture,2013,37(15):108.
[6]范林林,王清,高丽朴,等.二氧化氯对豇豆采后生理特性的影响[J].北方园艺,2015,39(24):122.[doi:10.11937/bfyy.201524034]
FAN Linlin,WANG Qing,GAO Lipu,et al.Effect of ClO2 Treatment on Quality of Postharvest Cowpea During Cold Storage[J].Northern Horticulture,2015,39(15):122.[doi:10.11937/bfyy.201524034]
[7]姚岭柏,韩海霞.盐胁迫对樱桃萝卜生长及生理生化指标的影响[J].北方园艺,2016,40(13):5.[doi:10.11937/bfyy.201613002]
YAO Lingbai,HAN Haixia.Effect of Salt Stress on Growth and Index of Physiology and Biochemical of Paphanus sativus L.var.radculus Pers.[J].Northern Horticulture,2016,40(15):5.[doi:10.11937/bfyy.201613002]
[8]车建美.,刘 波,张 彦l.,等.青枯雷尔氏菌胁迫对烟草植株抗氧化酶系活性的影响[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102046]
CHE Jian-mei.,LIU Bo,ZHANG Yan.,et al.Effects of Avirulent Ralstonia solanacearum Strain on Some Antioxidant Enzymes Activities in Tobacco Plants[J].Northern Horticulture,2011,35(15):0.[doi:10.11937/bfyy.201102046]
[9]杨 静,李小娟,王 丽,等.外源过氧化物酶对辣椒果实抗氧化酶活性与品质的影响[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108001]
YANG Jing,LI Xiao-juan,WANG Li,et al.Effect of External Peroxidase on the Activities of Antioxidant Enzymes in Pepper[J].Northern Horticulture,2011,35(15):0.[doi:10.11937/bfyy.201108001]
[10]李根柱,张自川,郑云普,等.五种北高丛蓝莓对干旱胁迫的生理响应及其耐旱性评价[J].北方园艺,2016,40(24):10.[doi:10.11937/bfyy.201624003]
LI Genzhu,ZHANG Zichuan,ZHENG Yunpu,et al.Physiological Response to Drought Stress and Drought Tolerance Evaluation of Five North High-bush Blueberry Varieties[J].Northern Horticulture,2016,40(15):10.[doi:10.11937/bfyy.201624003]
备注/Memo
作者简介：王娟（1977-），女，博士，副教授，现主要从事植物生物技术的教学与科研工作。E-mail：wangjuan310@163.com.