YANG Yuan,SHI Lei,ZHANG Jia,et al.Determination of Ellagic Acid in Strawberry by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2012,36(15):25-27.
分散固相萃取-高效液相色谱法测定草莓中游离鞣花酸含量
- Title:
- Determination of Ellagic Acid in Strawberry by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography
- 文章编号:
- 1001-0009(2012)15-0025-03
- Keywords:
- DSPE; HPLC; ellagic acid; strawberry
- 分类号:
- Q 946
- 文献标志码:
- A
- 摘要:
- 建立了分散固相萃取-高效液相色谱法（DSPE-HPLC）测定草莓中游离鞣花酸含量的检测方法。在酸性条件下用甲醇提取，经C18分散固相萃取净化。采用Agilent Poroshell 120 SB-C18柱（100 mm×4.6 mm，2.7 μm）分离，以乙腈-0.1%三氟乙酸水溶液（15∶85，V/V）为流动相，在254 nm下检测。该方法可以将鞣花酸与基质良好分离，在0.1～20 μg/mL浓度范围内线性良好（r = 0.99981）；最低检出浓度为0.0134 μg/g，回收率为85.3%~95.1%，相对标准偏差小于10%，可用于草莓中游离鞣花酸含量的检测。
- Abstract:
- An analytical method was developed to extract and determine content of free ellagic acid in strawberry using dispersive solid phase extraction and high performance liquid chromatography (DSPE-HPLC).The free ellagic acid in strawberry was extracted under 0.1% trifloroacetric acid-methane,and purified by C18 dispersive solid-phase extraction.Then strawberry sample was separated by column Poroshell 120 SB-C18 (100 mm×4.6 mm，2.7 μm) and measured at UV wavelength of 254 nm with the mobile phase consisting of acetonitrile∶water (0.1% trifloroacetric acid) (15∶85，V/V).The method could determine the free ellagic acid in strawberry and had fine linear relationship (r=0.99981) when the range of ellagic acid was within 0.1~20 μg/mL.Besides that，the minium detection concentration was 0.0134 μg/g，and average recoveries were 85.3%~95.1% with relative standard deviation (RSD，%)<10%.In summary，this approach using DSPE-HPLC was capable to determine free ellagic acid in strawberry.
参考文献/References:
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备注/Memo
第一作者简介：杨媛(1977-)，女，博士，副研究员，现主要从事果品质量与安全等研究工作。E-mail：yangyuan@mail.nankai.edu.cn.