SUN Hong-mei,LIU Du-ling,YANG Ji-an,et al.Response on Physiology and Biochemistry and Cold Resistance Evaluation of Precocity Walnut Cultivar Under Low Temperature Stress[J].Northern Horticulture,2012,36(15):17-20.
早实核桃对低温胁迫的生理响应及抗寒性评价
- Title:
- Response on Physiology and Biochemistry and Cold Resistance Evaluation of Precocity Walnut Cultivar Under Low Temperature Stress
- 文章编号:
- 1001-0009(2012)15-0017-04
- 分类号:
- S 664.1
- 文献标志码:
- A
- 摘要:
- 采用人工模拟低温环境的方法，以9 a生早实核桃“香玲”和“辽核4号”离体新梢为试材，在低温胁迫下，测定细胞质膜透性、丙二醛含量、可溶性糖含量、脯氨酸含量及SOD活性，并进行生长恢复试验，用相关分析确定评价早实核桃抗寒性的生理生化指标，用隶属函数法对2个品种抗寒性进行综合评价。结果表明：随温度降低，2个品种相对电导率呈“S”型曲线；丙二醛含量呈升-降-升的趋势；可溶性糖含量先升高后降低趋势；“香玲”和“辽核4号”脯氨酸含量分别呈升-降-升-降和先升后降的趋势；“香玲”和“辽核4号”SOD活性分别呈先升后降和升-降-升-降的趋势。确定相对电导率、半致死温度、可溶性糖含量和脯氨酸含量作为评价核桃抗寒性的指标；2个品种抗寒性综合评价结果为：“辽核4号”＞“香玲”。
- Abstract:
- The membrane permeability of 9 years old ‘Xiangling’ and ‘Liaohe No.4’ shoots，malondialdehyde content，the soluble sugar and praline content and SOD activity were investigated with the method of artificial simulation of low-temperature.Besides，the recovering growth situations were observed.Physiological-biochemical indicators were determined by correlation analysis and the cold resistance of the two walnut cultivars was evaluated by using fuzzy mathematics method.The results showed that the relative electric conductivity of the two walnut cultivars increased strongly with following an ‘S’ shape during the drops in temperature.The malondialdehyde content increased at first，then decreased and increased again.The soluble sugar showed a tendency of increasing at the initial stage of the low temperature and decreasing later.Praline content of ‘Xiangling’ increased at first，then decreased and then increased and decreased again and that of ‘Liaohe No.4’ showed a tendency of increasing at first and decreasing later.SOD activity of ‘Xiangling’ increased at the initial stage and decreased later and that of ‘Liaohe No.4’ increased at first，then decreased and then increased and decreased again.The conductivity，semilethal temperature，the soluble sugar and praline content were determined to evaluate the cold resistance of walnut cultivars.The cold-resistance of test walnut was evaluated as ‘Liaohe No.4’>’Xiangling’.
参考文献/References:
[1]郗荣庭,张毅萍.中国果树志·核桃卷[M].北京:中国林业出版社,1992:50-80. [2]相昆,张美勇,徐颖,等.不同核桃品种耐寒特性综合评价[J].应用生态学报,2011,22(9):2325-2330. [3]赵世杰,史国安.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002. [4]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [5]高俊凤.植物生理学实验技术[M].西安:世界图书出版公司，2000. [6]许瑛,陈发棣.菊花8个品种的低温半致死温度及其抗寒适应性[J].园艺学报,2008,35(4):559-564. [7]柳新红,何小勇,苏冬梅,等.翅荚木种源抗寒性综合评价体系的构建与应用[J].林业科学,2007,43(10):45-50. [8]吕跃东,董凤祥,王贵禧,等.平欧杂交榛抗寒性综合评级体系的建立与应用[J].林业科学,2008,44(9):31-35. [9]杨建民,周怀军,王文凤.果树霜冻害研究进展[J].河北农业大学学报,2000,23(3):54-58. [10]李轶冰,杨顺强,任广鑫,等.低温处理下不同禾本科牧草的生理变化及其抗寒性比较[J].生态学报,2009,29(3):1341-1347. [11]郭卫东,张真真,蒋小伟,等.低温胁迫下佛手半致死温度测定和抗寒性分析[J].园艺学报,2009,36(1):81-86. [12]邱乾栋.桂花对低温胁迫适应性的研究[D].泰安:山东农业大学,2010. [13]崔帅,赵兰勇,李承水,等.卫矛属3种常绿阔叶树木抗寒性研究[J].中国农学通报,2012,28(7):17-20. [14]刘晓东,翟晓宇,施冰.金叶风箱果和紫叶风箱果的抗寒性[J].东北林业大学学报,2011,39(4):18-20. [15]滕祥金,孙玉刚,吴姝菊,等.不同草地早熟禾品种抗寒性研究[J].北方园艺,2011(8):90-92. [16]王宁,吴军,夏鹏云,等.大叶冬青对低温胁迫的生理响应及抗寒性分析[J].华南农业大学学报,2011,32(3):82-86.
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备注/Memo
第一作者简介：孙红梅（1987-），女，在读硕士，研究方向为森林培育理论与技术。