SUN Fang,NIU Tian,LI Cheng-xiu,et al.Establishment of Rapid Propagation System of Hybrid Embryo Between Cymbidiumkanran Makino and Cymbidium faberi Rolfe[J].Northern Horticulture,2012,36(24):124-128.
寒兰与蕙兰杂交种胚快繁体系的建立
- Title:
- Establishment of Rapid Propagation System of Hybrid Embryo Between Cymbidiumkanran Makino and Cymbidium faberi Rolfe
- 文章编号:
- 1001-0009（2012）24-0124-03
- Keywords:
- hybrid Cymbidium; rhizome; proliferation times; differentiation rate
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 为加快杂交兰快繁体系的建立，以蕙兰‘新上海’（♂）与寒兰‘寒香梅’（♀）杂交种胚获得的根状茎为外植体，采用不同浓度NAA和6-BA配比组合，研究其对杂交兰增殖与分化的影响。结果表明：杂交根状茎在MS+1.0 mg/L NAA+0.1 mg/L 6-BA的基本培养基中增殖效果最好，增值倍数达到3.08。在培养基MS+0.1 mg/L NAA+5 mg/L 6-BA的培养基上分化产生的不定芽数量最多。1/2MS+1.0 mg/L NAA+0.2%AC培养条件下，不定根得到很好诱导。激素配比可以很好的加快杂交兰的快速繁殖。
- Abstract:
- In order to speed up the building of the rapid propagation system for hybrid cymbidium，taking rhizome of ‘Xinshanghai’(♂)בHanxiangmei’(♀) as explants,the effects of different concentrations of 6-BA and NAA combinations on rapid propagation and differention were studied.The results showed that MS+1.0 mg/L NAA+0.1 mg/L 6-BA was the highest propagation coefficient and it could reach 3.08.MS+0.1 mg/L NAA+5 mg/L 6-BA was the optimum medium for adventitious bud regeneration.1/2MS+1.0 mg/L NAA+0.2%AC could be good for adventitious roots’ induction.The use of hormone would speed up the rapid propagation of hybrid Cymbidium.
参考文献/References:
[1] 李少球 , 胡松华 , 鲁章 . 中国兰花 ( 品种 欣赏 栽培)[M].广州:广东科技出版社,2003. [2]卢思松.中国兰与洋兰[M].北京:金盾出版社,1994. [3]梁芳,崔波,马杰,等.杂交兰原球茎增殖及分化研究[J].安徽农业科学,2008,36(13):5309-5310,5353. [4]孙芳,李承秀,张林,等.春兰名品杂交后代快繁与分化研究[J].中国农学通报,2012,28(10):189-193. [5]朱国兵,杨柏云,蔡奇英,等.寒兰的快速繁殖技术[J].热带亚热带植物学报,2006,14(2):151-156. [6]朱根发,陈明莉,罗智伟,等.墨兰与大花蕙兰种间杂种原球茎的诱导及增殖研究[J].园艺学报,2004,31(5):688-690. [7]周全,余平.春兰根状茎的增殖与分化条件优化[J].湖北农业科学,2009,48(1):27-30. [8]徐程,詹忠根.中国兰的组织培养[J].植物生理学通讯,2002,38(2):171-174. [9]褚云霞,张永春.春兰根状茎增殖与分化培养[J].上海农业学报,2007,23(3):82-85. [10]杨柏云,杨宁生.蕙兰原球茎增殖培养条件的研究[J].南昌大学学报,1995,19(1):39-42. [11]朱根发,王碧青,吕复冰.建兰与纹瓣兰种间杂种胚培养研究[J].热带亚热带植物学报，2005,13(5):447-450. [12]石乐娟,张放,张士良,等.植物生长调节剂对线艺春兰根状茎的增殖与分化的影响[J].园艺学报,2006,33(4):887-890. [13]陈少珍,卜朝阳,闭志强,等.兰花组织培养中常见问题及解决方法[J].广西农业科学,2006,37:72-74. [14]罗毅波.国兰产业化发展中的几个问题[J].中国西部科技,2006(15):14-17. [15]张孟锦,杨志娟,许大熊,等.兰花育种途径与进展[J].广东农业科学,2008(2):120-123.
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备注/Memo
第一作者简介：孙芳（1987-），女，山东潍坊人，在读硕士，研究方向为园林植物遗传育种。E-mail:yufang12221987@126.com.