YANG Gao-qiang,HUO Xiu-wen.Differentially Distinguished Expressed Genes in Chili Pepper Genic Male Sterility(GMS) Using cDNA-AFLP[J].Northern Horticulture,2012,36(13):132-136.
利用cDNA-AFLP研究辣椒细胞核雄性不育差异表达基因
- Title:
- Differentially Distinguished Expressed Genes in Chili Pepper Genic Male Sterility(GMS) Using cDNA-AFLP
- 文章编号:
- 1001-0009（2012）13-0132-05
- Keywords:
- chili pepper; genic male sterility; cDNA-AFLP
- 分类号:
- S 641.303.6
- 文献标志码:
- A
- 摘要:
- 辣椒细胞核雄性不育两用系在辣椒杂交种配制方面有着非常重要的应用，为了进一步了解其不育性的分子机理，利用cDNA-AFLP对辣椒细胞核雄性不育两用系‘AB23’可育株和不育株花蕾的mRNA指纹图谱进行了初步分析。结果表明：共获得19个差异表达的TDF，均来自可育株。在可育株花蕾中得到了19个TDFs，其中13个TDFs功能已知，2个功能未知，4个没有找到同源序列。对13个已知序列的分析表明，这些基因涉及了细胞的代谢、转录、胞间运输和信号转导等相关过程。通过半定量RT-PCR技术对获得的19个TDFs进行验证，其表达特征与cDNA-AFLP结果一致。
- Abstract:
- Genic male sterility is an important way to utilize the heterosis of chili pepper,So,pilot studies were conducted for better understanding the mechanism of the chili pepper GMS，establish chili fertile plants buds and sterility plant buds cDNA library between the homozygous genic male sterility two-type line ‘AB23’ and by using cDNA-AFLP in chili pepper genic male sterility of sterility genes for preliminary research.The results showed that there were 19 bands expressed in a higher level in fertile plant buds，Among them,13 bands homologous sequences function known,2 bands function unknown and 4 bands were maybe some novel genes.The 13 homologous sequences function known participated in the following biological process：metabolism，transcription，cellular transport，signal transduction mechanism etc.Furthermore,RT-PCR demonstrates that it was consistent with the result of cDNA-AFLP.This study result will help us understand that the development of chili pepper male gametes and could give basic information about the genic male sterility in chili pepper.
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备注/Memo
第一作者简介：杨高强（1979-），男，硕士，农艺师，现主要从事辣椒遗传育种研究工作。E-mail:gaoqiang0120@163.com.
责任作者：霍秀文(1968-)，女，博士，教授，硕士生导师，现主要从事蔬菜种子资源与遗传育种研究工作。E-mail:xiuwenhuo@yahoo.com.
基金项目：沈阳市科技计划资助项目（F10-077-3-00）。
收稿日期：2012-03-07