KUANGNing-ning,ZHANGRu-qin,YANHong-hai.EvaluationofResistanceandAnalysisofResistancePhysiologyinVarietiesof ChineseCabbageInfectedbyPlasmodiophorabrassicae[J].Northern Horticulture,2012,36(11):140-143.
大白菜品种间根肿病抗性鉴定及抗性生理分析
- Title:
- EvaluationofResistanceandAnalysisofResistancePhysiologyinVarietiesof ChineseCabbageInfectedbyPlasmodiophorabrassicae
- 文章编号:
- 1001-0009（2012）11-0140-04
- 分类号:
- S436.341.1+9
- 文献标志码:
- A
- 摘要:
- 通过苗期人工接种抗性鉴定、寄主防御酶活性和抗病相关物质含量变化测定，研究大白菜品种对根肿病的抗性及根肿病对大白菜生长的影响。结果表明：13个大白菜品种中‘87-114’较为抗病，“夏抗王”较为感病，没有免疫品种；大白菜植株接种根肿菌后，抗感品种的PAL、SOD、PPO、POD活性和Pro、MDA含量均呈不同程度的升高，且多数防御酶具有单峰型酶活高峰，活性提高幅度因酶而异。抗病品种寄主防御酶活性较感病品种增加幅度大，并且酶活峰值出现的时间也早于感病品种。
- Abstract:
- TheexperimentfocusedontheresearchofresistanceandgrowthofdifferentvarietiesofChinesecabbagesinfectedbyPlasmodiophorabrassicae,accordingtoartificialvaccinationofseedingstageandthevariationofdefensiveenzymesandsubstancesrelevanttothehostdefense.Theresultsshowedthat‘87-114’wasmoreresistantthanothers,‘Xiakangwang’wasmoresusceptibletoPlasmodiophorabrassicae，andthekindofcompleteimmunitywasnotfoundoutfromthetestrace.Forresistantandsusceptiblevarieties，theactivitiesofPAL,SOD,PPO,PODandthecontentsofPro,MDAwereallincreasedindifferentdegreeafterinoculatingthePlasmodiophorabrassicae.Themostofdefensiveenzymesexistedtheunimodalpeakofenzymeactivity,andtheamplitudeofimprovementindifferentenzymewasdifferentaswell.Theactivityofresistantvarietycanbeincreasedtoahigherextentthansusceptibleone,andthepeakappearedearlierthanthatofsusceptible.
参考文献/References:
[1]李淼,檀根甲,承河元,等.植物抗病性研究现状与前景展望[J].江西农业大学学报,2002,24(5):731-736.
[2]ChiangMS,CreteR.Inheritanceofclubrootresistanceincabbage(BrassicaoleraceaL.var.capitataL.)[J].Can.J.Genet.Cytol.,1970,12:253-256.
[3]符明连,杨玉珠,李根泽,等.不同油菜品种对根肿病的抗性分析[J].华中农业大学学报,2011,30(4):443-447.
[4]李晓鸥,吴飞燕,钟惠宏,等.大白菜根肿病苗期人工接种抗病性鉴定技术初报[J].中国蔬菜,1990(6):9-10.
[5]陈群航,黄建都,陈仁,等.大白菜品种对根肿病抗性鉴定研究[J].现代农业科技,2011(13):116-120.
[6]司军,李成琼,宋洪元,等.结球甘蓝对根肿病的抗性鉴定与评价[J].西南大学学报,2009,31(6):27-31.
[7]王伟玲,王展,王晶英,等.植物过氧化物酶活性测定方法优化[J].实验室研究与探索,2010,29(4):21-23.
[8]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:132-135,140-144.
[9]张梅,孙治军,王鸿磊,等.L型蜡样芽孢杆菌对草莓防御酶活性变化的影响[J].北方园艺,2011(9):43-45.
[10]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:248-249,260-261.
[11]蔡丽,王延丽,林春花,等.不同寄主多主棒孢对橡胶树叶片组织防御酶活性的影响[J].热带农业科学,2011,31(4):33-38.
[12]梁谊,张爱芳,王文相,等.十字花科蔬菜根肿病研究现状[J].安徽农业科学,2001,29(6):746-749.
[13]祝朋芳,赵妮拉,段玉玺,等.栽培菊感染菊花白锈病后体内防御酶活性的研究[J].北方园艺,2011(6):77-80. [14]CachineroJM,CabelloF,JorrinJ.Inductionofdifferentchitinaseandbeta1,3-glucanaseisoenzymesinsunflowerseedlingsinresponsetoinfectionbyplasmoparahalsted[J].EuropeanJournalofPlantPathology,1996,102(4):401-405.
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备注/Memo
第一作者简介：况宁宁（1984-）,女，在读硕士，研究方向为植物和病原物互作机制。
责任作者：鄢洪海（1964-）,男，博士，教授，研究方向为植物和病原物互作机制。E-mail:hhyan@qau.edu.cn。
基金项目：山东省科技攻关资助项目（2009GG10009022）。
收稿日期：2012-03-15