LIZheng,WANGYu,HAOLi-min.OptimizationofMediumforImprovingExo-polysaccharideofGrifolafrondosaby ResponseSurfaceMethodology[J].Northern Horticulture,2012,36(11):113-116.
利用响应面法优化灰树花胞外多糖的培养基
- Title:
- OptimizationofMediumforImprovingExo-polysaccharideofGrifolafrondosaby ResponseSurfaceMethodology
- 文章编号:
- 1001-0009（2012）11-0113-04
- 分类号:
- S646.2
- 文献标志码:
- A
- 摘要:
- 为了提高灰树花胞外多糖的产量，应用响应面分析方法对灰树花培养基进行了优化。通过对模型方程的响应曲面图及其等高线图进行分析。结果表明：影响该菌发酵产糖的优化培养基（g/L）：KH2PO42、MgSO41、VB10.02、麦麸50、葡萄糖20、玉米浆23.8、玉米粉19.7、豆饼粉5.2。在该培养基下胞外多糖产量为2.44g/L,比优化前的培养基产量提高了75.22%。
- Abstract:
- Inordertoincreasetheproductionofexo-polysaccharideofGrifolafrondosa,theoptimizationofmediumbyresponsesurfacemethodology(RSM)wasstudied.Theresultsshowedtheoptimalmediumwas(g/L)：KH2PO42,MgSO41,VB10.02,wheatbran50,glucose20,cornsteepliquor23.8,cornpowder19.7,soybeancakepowder5.2.Underthemedium,theexo-polysaccharideofGrifolafrondosawas2.44g/L,whichwasincreased75.22%thanthatofinitialmedium.
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备注/Memo
第一作者简介：李政（1978-），男，博士，讲师，现主要从事纺织相关生物发酵方面的研究工作。E-mail：lizheng@tjpu.edu.cn。
基金项目：国家自然科学基金资助项目（21006072,31171662）。
收稿日期：2012-03-07