ZHENG Su-yue,HAO Jun-ling,WANG Jian-wei.The Influences of Subculture of Cordceps militaris to Mycelial Growth Rate and Esterase Isozyme[J].Northern Horticulture,2012,36(10):136-137.
继代培养对蛹虫草菌丝生长速度及酯酶同工酶的影响
- Title:
- The Influences of Subculture of Cordceps militaris to Mycelial Growth Rate and Esterase Isozyme
- 文章编号:
- 1001-0009（2012）10-0136-02
- Keywords:
- Cordceps militaris; subculture; mycelial growth rate; esterase
- 分类号:
- S 567.3+9
- 文献标志码:
- A
- 摘要:
- 以蛹虫草菌株为试材，通过垂直板聚丙烯酰胺凝胶电泳和菌丝生长速度测定，对不同代次蛹虫草菌丝生长速度和菌株酯酶同工酶表现型进行比较分析，旨在寻找蛹虫草菌种退化机理。结果表明:不同代次蛹虫草的菌丝生长速度差异不显著，而酯酶同工酶酶谱发生较大变异。9个菌株共有3条迁移率不同的条带,分别为0.63、0.65和0.67,其中F1、F3、F4和F5代菌株出现第2、3条酶带,迁移率分别为0.65和0.67，而F2、F6、F7、F8、F9代菌株发生变异,迁移率为0.67的条带消失,菌株酶谱条带中产生了迁移率为0.63的新条带。
- Abstract:
- Mycelial growth rate and esterase isozyme of Cordceps militaris were tested by vertical slab polyacrylamide gel electrophoresis and mycelial growth rate determination,the aim was to search for the degradation mechanism of Cordceps militaris.The results showed that different esterase isozymes were observed among the different subculture strains and no difference in mycelial growth rates.EST isoenzyme showed that 3 EST bands of migration rates 0.63，0.65 and 0.67 were found in all tested strains.F1，F3,F4 and F5 strains showed 2 EST bands，the second and the third band，and the EST bands of F2,F6,F7,F8,F9varied，the band of migration rate 0.67 disappeared and a new band of 0.63 appeared.
参考文献/References:
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备注/Memo
第一作者简介：郑素月（1969-），女，河北石家庄人，博士，教授，现主要从事食用菌教学与科研工作。基金项目：河北省科技支撑计划资助项目（06220116D）。
收稿日期：2012-03-07