YOU Chao,ZHAO Da-qiu,LIANG Cheng-bang,et al.Cloning and Sequence Analysis for Farnesyl Diphosphate Synthase from Persimmon[J].Northern Horticulture,2012,36(09):105-108.
柿法呢基焦磷酸合酶基因的克隆与序列分析
- Title:
- Cloning and Sequence Analysis for Farnesyl Diphosphate Synthase from Persimmon
- 文章编号:
- 1001-0009（2012）09-0105-04
- Keywords:
- persimmon; farnesyl diphosphate synthase; cloning; sequence analysis
- 分类号:
- Q 946.8
- 文献标志码:
- A
- 摘要:
- 在萜类生物合成途径中起重要作用的关键酶—法呢基焦磷酸合酶（Farnesyl Diphosphate Synthase，FPS），对植物的生长发育、抗逆性以及次生代谢都起着重要的调节作用。通过cDNA末端快速克隆的技术（Rapid-amplification of cDNA Ends，RACE）从柿果实中克隆到法呢基焦磷酸合酶基因cDNA片段，并进行了序列的相关分析。结果表明：所克隆的FPS基因和NCBI数据库中已登录的云杉、腊梅、百合等植物FPS基因核苷酸序列的同源性分别达到88%、75%、73%，并且与香蕉、水稻、橄榄等植物FPS基因氨基酸序列的同源性分别达到72%、67%、66%。该基因已提交GenBank数据库，登录号为JN108022。
- Abstract:
- Farnesyl diphosphate synthase (FPS) is a key enzyme in the biosynthetic pathway of terpenes，it plays an important regulatory role in plant growth，stress resistance and secondary metabolism.The cDNA fragment of FPS was cloned from persimmon fruit by RACE method，and sequence analysis was carried out.The results indicated that the nucleotide sequence DkFPS cDNA fragment was homologus to Picea abies，Chimonanthus praecox and Lilium longiflorum with percentage of 88%,75% and 73%，respectively.The homology of amino acid sequence deduced from this cDNA fragment of DkFPS was 72%，67% and 66% to Musa acuminate，Oryza sativa Japonica and Olea europaea,respectively.This gene had been submitted to GenBank database with accession number JN108022.
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备注/Memo
第一作者简介：尤超（1987-），男，安徽灵璧人，硕士，现主要从事银杏次生代谢调控研究工作。E-mail：kyzy518529@163.com。
责任作者：周春华（1974-），男，浙江江山人，博士，副教授，硕士生导师，现主要从事园艺植物有效成分生物代谢及成分分离提取与应用研究工作。E-mail： chzhou@yzu.edu.cn。
基金项目：国家自然科学基金资助项目（30771491）；中国博士后科学基金资助项目(20100471401)；江苏省博士后科学基金资助项目(0901055C)；江苏省科技基础设施建设计划（科技公共服务平台）资助项目（BM2010590）。
收稿日期：2012-02-27