ZHANG Zhi-xin,ZHANG Shi-xiu.Infrared Spectrum Analysis of Extracts of Panax notoginseng Stems and Leaves[J].Northern Horticulture,2012,36(08):178-183.
三七茎叶提取物红外光谱分析
- Title:
- Infrared Spectrum Analysis of Extracts of Panax notoginseng Stems and Leaves
- 文章编号:
- 1001-0009(2012)08-0178-06
- 分类号:
- S 567.23+6
- 文献标志码:
- A
- 摘要:
- 以三七茎叶为原料,采用醇提水沉-D101大孔吸附树脂吸附、乙醇溶液梯度洗脱，得到不同洗脱物；在此基础上通过红外光谱法(FTIR)、二阶导数红外光谱法分析不同乙醇溶液洗脱物的“宏观指纹特征”，并与原人参二醇型皂苷对照品Rb1、黄酮类化合物对照品槲皮素、芦丁的红外光谱进行比较。结果表明：不同洗脱物的一维红外光谱及二阶导数红外光谱有明显差异，初步指认了不同洗脱物的吸收特征峰，30%、50%的乙醇溶液洗脱物主要成分为三七茎叶皂苷，并且2个活性物的成分不同；60%、70%、90%乙醇溶液洗脱物主要成分为黄酮类化合物，并且结构有差异，可以作为不同的药理活性成分进行药物开发，为三七茎叶原料药的提取建立分析模式。
- Abstract:
- Taking Panax notoginseng stems and leaves as the materials，different elution fractions were prepared by precipitating with alcohol extracting-water，adsorption with D101 macroporous resin and gradient elution with ethanol solution.Then analyzing ‘macro fingerprint characteristics’ of different elution fractions by Fourier Transform infrared spectroscopy and second serivative infrared spectroscopy.The results were compared with infrared spectroscopy ofprotopanaxadiol saponins Rb1 reference substance，flavonoids quercetin reference substance and rutin.The results showed that one-dimensiona infrared spectroscopy and second serivative infrared spectroscopy of different elution fractions were different.The characteristic absorption peaks of different elution fractions were described.The elution fractions with 30% and 50% ethanol solution mainly were different component saponins of Panax notoginseng stems and leaves.The elution fractions with 60%，70% and 90% ethanol solution mainly were flavonoids with different structure.These products can be used as different pharmacological component to meet the challenge of drug discovery and establishing analysis pattern for extraction of Panax notoginseng stems and leaves crude drug.
参考文献/References:
[1]魏均娴,杜元冲.三七-现代科学研究与应用[M].昆明:云南科技出版社,1996:31-34.
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备注/Memo
第一作者简介：张志信（1970-），男，硕士，副教授，现主要从事天然药物等研究工作。