LI Li-xia,FU Zhen-fang,HE Hui-qiang,et al.Physilogical Response of Cucumber Seedlings After Pseudoperonospora cubensis Inoculation[J].Northern Horticulture,2012,36(05):25-27.
不同抗性的黄瓜幼苗对接种霜霉病菌的生理响应
- Title:
- Physilogical Response of Cucumber Seedlings After Pseudoperonospora cubensis Inoculation
- 文章编号:
- 1001-0009(2012)05-0025-03
- Keywords:
- cucumber; downy mildew; MDA; soluble sugar; soluble protein; ethylene
- 分类号:
- S 642.204+.3
- 文献标志码:
- A
- 摘要:
- 以黄瓜抗病品种“津春4号”和感病品种“长春密刺”为试材,在接种黄瓜霜霉病菌后0.5、1、2、4、7 d对叶片的丙二醛(MDA)、可溶性糖、可溶性蛋白含量和乙烯释放量进行了检测,并分析了这些生理变化与黄瓜霜霉病抗性之间的关系。结果表明:抗病品种黄瓜叶片中MDA含量、乙烯释放量低于感病品种,而可溶性糖、可溶性蛋白含量则高于感病品种。表明抗病品种比感病品种具有更强的防御能力和抗膜脂过氧化能力。
- Abstract:
- Taking cucumber resistant variety ‘Jinchusihao’ and susceptible variety ‘Changchunmici’ as test material,the changes of malondialdehyde(MDA),soluble sugar,soluble protein and ethylene content on cucumber seedlings after inoculated with Pseudoperonospora cubensis 0.5,1,2,4,7 d were analyzed,and relations between these physiogical changes and resistance against downy mildew were also studied.The results showed that the contents of MDA and ethylene in resistant variety were lower,while the contents of soluble sugar and soluble protein were higher than that of susceptible variety.It demonstrated that resistant variety possessed stronger protective ability and was more resistant to membrane lipid peroxidation.
参考文献/References:
[1]邢彩云,胡锐,李丽霞,等.黄瓜霜霉病的发生与综合防治[J].中国瓜菜,2011,24(2):56-58.
[2]李玉红,程智慧,陈鹏,等.苯并噻二唑(BTH)诱导黄瓜幼苗对霜霉病抗性的研究[J].园艺学报,2006,33(2):278-282.
[3]刘艳玲,张艳菊,蔡宁,等.黄瓜霜霉病病原与抗病性研究进展[J].东北农业大学学报,2009,40(4):127-131.
[4]王丽娟,孙彩玉,牛德,等.黄瓜抗霜霉病的分子生物学研究进展[J].中国蔬菜,2010(24):10-13.
[5]李宝聚,彭仁,彭霞薇,等.高温调控对黄瓜霜霉病菌侵染的影响[J].生态学报,2001,21(11):1796-1801.
[6]孙群,胡景江.植物生理研究技术[M].杨凌:西北农林科技大学出版社,2006:165-170.
[7]邓军,林秀琴,韦焕琪,等.毛细管柱气象色谱法测定橡胶树乳胶中的乙烯含量及变化[J].福建农林大学学报(自然科学版),2009,38(6):600-602.
[8]陈毓荃.生物化学实验方法与技术[M].北京:科学出版社,2002:71-73.
[9]房保海,张广民,迟长凤,等.烟草低头黑病菌毒素对烟草丙二醛含量和某些防御酶的动态影响[J].植物病理学报,2004,34(1):27-31.
[10]王建明,张作刚,郭春绒,等.枯萎病菌对西瓜不同抗感品种丙二醛含量及某些保护酶活性的影响[J].植物病理学报,2001,31(2):152-156.
[11]刘庆元,张穗,李久禄,等.黄瓜品种对霜霉病的抗性机理[J].华北农学报,1993,8(1):70-75.
[12]刘素萍,王汝贤,张荣,等.根系分泌物中糖和氨基酸对棉花枯萎菌的影响[J].西北农业大学学报,1998,26(6):30-35.
[13]Van Loon Leendert C,Geraats Bart P J,Linthorst Huub J M.Ethylene as a modulator of disease resistance in plants[J].Trends in Plant Science,2006,11(4):184-191.
[14]Chagué V,Danit LV,Siewers V,et al.Ethylene sensing and gene activation in Botrytis cinerea:a missing link in ethylene regulation of fungus-plant interactions Mol[J].Plant Microbe Interact,2006,19:33-42.
[15]Weingart H,Ullrich H,Geider K,et al.The role of ethylene production in virulence of Pseudomonas syringae pvs.glycinea and phaseolicola[J].Phytopathology,2001,91:511-518.
相似文献/References:
[1]李亚芹,郑根昌.冬棚黄瓜的氮磷钾肥配施效应分析[J].北方园艺,2014,38(12):146.
LI Ya-qin,ZHENG Gen-chang. Effect Analysis of Application of N,P and K Fertilizers on Cucumber in Winter Plastic Greenhouses [J].Northern Horticulture,2014,38(05):146.
[2]由玉卿,秦智伟,周秀艳,等.黄瓜果实中丙醇二酸含量的配合力分析[J].北方园艺,2014,38(10):19.
YOU Yu-qing,QIN Zhi-wei,ZHOU Xiu-yan,et al.Analysis on Combining Ability of Tartronic Acid Content in Cucumber[J].Northern Horticulture,2014,38(05):19.
[3]张秋萍,祁红英,张 珏,等.丛枝菌根制剂对黄瓜幼苗生长的影响[J].北方园艺,2014,38(14):37.
ZHANG Qiu-ping,QI Hong-ying,ZHANG Yu,et al.Effect of Arbuscular Mycorrhizal Agent on Growth of Cucumber Seedling[J].Northern Horticulture,2014,38(05):37.
[4]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(05):1.
[5]王昶童,曹刚强,梁丛敏,等.黄瓜NBS类抗病基因类似物的生物信息学分析[J].北方园艺,2014,38(14):101.
WANG Chang-tong,CAO Gang-qiang,LIANG Cong-min,et al.Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber[J].Northern Horticulture,2014,38(05):101.
[6]张瑞芬,李红岭,李新旭,等.优比胶囊不同使用方法对黄瓜生长及产量的影响[J].北方园艺,2013,37(17):4.
ZHANG Rui-fen,LI Hong-ling,LI Xin-xu,et al.Effect of Different Usage of UB Capsule on Growth and Yield of Cucumber[J].Northern Horticulture,2013,37(05):4.
[7]张雪艳,李堃,马丽娟,等.Ca2+调控对黄瓜幼苗耐盐性生理指标的影响[J].北方园艺,2013,37(13):5.
ZHANG Xue-yan,LI Kun,MA Li-juan,et al.Effects of Calcium Regulation on the Physiological Properties of Cucumber Under Salt Stress[J].Northern Horticulture,2013,37(05):5.
[8]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination,Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(05):9.
[9]张建祥,贾利元.越冬茬黄瓜结瓜期管理技术[J].北方园艺,2013,37(13):63.
ZHANG Jian-xiang,JIA Li-yuan.Management Techniques of Overwintering Cucumber During the Fruiting Period[J].Northern Horticulture,2013,37(05):63.
[10]宁雪飞,韩小路,王贤磊,等.一种快速筛选转抗霜霉病基因甜瓜种子的方法研究[J].北方园艺,2014,38(01):102.
NING Xue-fei,HAN Xiao-lu,WANG Xian-lei,et al.The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon[J].Northern Horticulture,2014,38(05):102.
[11]刘高峰.脱落酸诱导黄瓜对霜霉病的抗性研究[J].北方园艺,2012,36(02):146.
LIU Gao-feng.Efficiency of Cucumber Resistance Induced by ABA to Pseudoperonospora cubensis[J].Northern Horticulture,2012,36(05):146.
[12]张亚莉,郭玉炜,周桂荣,等.中量元素肥料对黄瓜生长发育的影响[J].北方园艺,2012,36(11):153.
ZHANGYa-li,GUOYu-wei,ZHOUGui-rong,et al.EffectofMediumConcentrationElementFertilizeronGrowthofCucumber[J].Northern Horticulture,2012,36(05):153.
[13]秦雪峰,梁海燕,余昊,等.霜霉病对大棚设施黄瓜光合特性的影响[J].北方园艺,2015,39(17):99.[doi:10.11937/bfyy.201517025]
QIN Xuefeng,LIANG Haiyan,YU Hao,et al.Effect of Downy Mildew on Photosynthesis Characteristics of Cucumber in Greenhouse[J].Northern Horticulture,2015,39(05):99.[doi:10.11937/bfyy.201517025]
[14]赵恒栋,崔世茂,刘金泉,等.叶片酸碱度对黄瓜霜霉病防控效果的研究[J].北方园艺,2010,34(03):0.[doi:10.11937/bfyy.201003081]
ZHAO Heng-dong,CUI Shi-mao,LIU Jin-quan,et al.Effect of Adjusting Leaves pH on Preventing and Controlling Cubensis[J].Northern Horticulture,2010,34(05):0.[doi:10.11937/bfyy.201003081]
[15]张丽莉,李涛,曹守军,等.烟台地方黄瓜种质资源抗病性鉴定与筛选[J].北方园艺,2017,41(18):11.[doi:10.11937/bfyy.20170836]
ZHANG Lili,LI Tao,CAO Shoujun,et al.Disease Resistance Identification and Screening of Yantai Cucumber Germplasm[J].Northern Horticulture,2017,41(05):11.[doi:10.11937/bfyy.20170836]
[16]高亚轻,苑磊,种道齐,等.不同时长UVA照射对黄瓜霜霉病发生和生理特征的影响[J].北方园艺,2019,43(17):1.[doi:10.11937/bfyy.20184672]
GAO Yaqing,YUAN Lei,CHONG Daoqi,et al.Effects of Different Duration of UVA Irradiation on the Occurrence and Morphological Characteristics of Cucumber Downy Mildew[J].Northern Horticulture,2019,43(05):1.[doi:10.11937/bfyy.20184672]
备注/Memo
第一作者简介:李丽霞(1986-),女,山西晋城人,在读硕士,研究方向为蔬菜生物技术与育种。
责任作者:李玉红(1973-),女,副教授,硕士生导师,现主要从事蔬菜种质资源与遗传育种等研究工作。
基金项目:国家自然科学基金资助项目(31071791);西北农林科技大学基本科研业务费专项资金资助项目(QN2009011)。
收稿日期:2011-12-28