SHI Long-wen,WANG Xia,LU Jia.The Bacteriostatic Effect of ‘Yipeiling’ and Its Influence on Multiplication and Differentiation of Protocorm Like-body of Cymbidium hybridum[J].Northern Horticulture,2012,36(04):123-125.
“益培灵”的抑菌效果及对大花蕙兰类原球茎增殖与分化的影响
- Title:
- The Bacteriostatic Effect of ‘Yipeiling’ and Its Influence on Multiplication and Differentiation of Protocorm Like-body of Cymbidium hybridum
- 文章编号:
- 1001-0009（2012）04-0123-03
- Keywords:
- ‘Yipeiling’; bacteriostatic effect; Cymbidium hybridum; protocorm like-body(PLB); multiplication and differentiation
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 将被萎蔫短小杆菌污染的大花蕙兰的类原球茎置于MS培养基中，以添加不同浓度、不同处理方法的“益培灵”杀菌剂进行正交实验，并观察类原球茎的平均增殖倍数及芽与根的生长分化情况。结果表明：“益培灵”对培养后期的萎蔫短小杆菌基本无抑菌效果。由于污染程度有差异，引起类原球茎平均增殖倍数、芽与根的生长数及分化率的差异。以培养基中添加0.05 g/L“益培灵”和消毒液浸泡6 min组合的类原球茎增殖倍数高，芽和根的生长数多，分化率高。
- Abstract:
- The protocorm like-body(PLB) of Cymbidium hybridum contaminated by Curtobacterium flaccumfaciens pv. betae/oortii was put in MS medium，and factorial experiment with different level (concentration or treatment) of ‘Yipeiling’ was carried out，then results of average multiplicative times，the growth and differentiation of sprout and root of PLB were observed.The results showed that ‘Yipeiling’ had not inhibiting effect to Curtobacterium flaccumfaciens pv. betae/oortii during later period.It caused the difference among different checks of average multiplicative times，the growth quantity and differentiation rate of sprout and root of PLB due to the different contaminated degree.The combination of MS medium added 0.05 g/L of ‘Yipeiling’ and PLB dipped in thimerosal in 6 minutes was better，and its average multiplicative time of PLB was higher，the growth quantity of sprout and root of PLB was more and the differentiation rate of them was higher.
参考文献/References:
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[4]张晓梅，林友伟，吴新华，等.菜豆萎蔫病菌的PCR检测技术[J].南京农业大学学报，2004，27(3):42-45.
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备注/Memo
第一作者简介：施隆文(1951-)，男，浙江宁波人，本科，副教授，现主要从事植物组织培养和植物保护的教学和研究工作。E-mail:slw51@126.com。
基金项目：嘉兴市科技局资助项目(2008AY1002)。
收稿日期：2011-11-21