[1]孙彩艺,陈静,侯蓉苗,等.激动素对肾蕨切叶活性氧代谢及抗氧化系统的影响[J].北方园艺,2023,(07):61-69.[doi:10.11937/bfyy.20223615]
　SUN Caiyi,CHEN Jing,HOU Rongmiao,et al.Effects of Kinetin on Reactive Oxygen Species Metabolism and Antioxidant System in Cut Foliage of Nephrolepis cordifolia L.[J].Northern Horticulture,2023,(07):61-69.[doi:10.11937/bfyy.20223615]

点击复制

激动素对肾蕨切叶活性氧代谢及抗氧化系统的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年07 页码: 61-69 栏目: 出版日期: 2023-04-15

Title:: Effects of Kinetin on Reactive Oxygen Species Metabolism and Antioxidant System in Cut Foliage of Nephrolepis cordifolia L.

作者:: 孙彩艺1; 陈静1; 侯蓉苗1; 徐文艺1; 杨丽芝1; 张璐1; 2; （1.东北农业大学园艺园林学院，黑龙江哈尔滨 150000；2.浙江农林大学风景园林与建筑学院，浙江杭州 310000）

Author(s):: SUN Caiyi1; CHEN Jing1; HOU Rongmiao1; XU Wenyi1; YANG Lizhi1; ZHANG Lu1; 2; (1.College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150000；2.College of Landscape Architecture and Architecture,Zhejiang A&F University,Hangzhou,Zhejiang 310000)

关键词:: 细胞分裂素; 激动素; 活性氧; AsA-GSH循环; 肾蕨

Keywords:: cytokinin; kinetin; reactive oxygen species; AsA-GSH cycle; Nephrolepis cordifolia L

DOI:: 10.11937/bfyy.20223615

文献标志码:: A

摘要:: 以肾蕨为试材，采用瓶插的方式，研究了激动素（KT）溶液[5、7.5、10 μmol?L-1（KT5、KT7.5、KT10）]作为瓶插液对肾蕨切叶活性氧代谢及抗氧化系统的影响，以期阐明激动素延缓肾蕨切叶衰老的机理。结果表明：瓶插12 d后，与对照（去离子水瓶插）相比，KT10处理的切叶过氧化氢、超氧阴离子和丙二醛含量以及电导率均降低，分别降低了92.06%、20.72%、410.34%、104.40%，其次为KT7.5（-46.46%、-18.09%、-173.39%、-59.93%）和KT5（-23.69%、-13.40%、-63.58%、-37.39%），说明KT可以抑制活性氧的产生，维持细胞膜的完整性及稳定性。KT10瓶插提高了超氧化物歧化酶(SOD)（19.32%）和过氧化氢酶(CAT)（86.36%）活性，其次为KT7.5，增加了13.37%和42.42%，KT5瓶插仅增加了7.76%和31.82%。同时，KT10处理还通过维持抗坏血酸过氧化物酶(APX)（225%）、谷胱甘肽还原酶(GR)（366.67%）、脱氢抗坏血酸还原酶(DHAR)（566.67%）活性来维持AsA-GSH循环的平衡，降低了总抗坏血酸（-442.12%）和总谷胱甘肽（-27.90%）含量，缓解了氧化损伤。KT7.5和KT5也在一定程度上维持AsA-GSH循环的平衡。总之，激动素能通过抑制活性氧含量、维持膜稳定性、激活抗氧化防御机制以及平衡AsA-GSH循环，有效延缓切叶的衰老，而抗衰老的效果为KT10>KT7.5>KT5。

Abstract:: The Nephrolepis cordifolia L.was used as test material.The effects of kinetin (KT) solution with different concentrations [5,7.5,10 μmol?L-1 (KT5,KT7.5,KT10)] on the vase life,reactive oxygen species metabolism and antioxidant system of cut foliage of Nephrolepis cordifolia L.were studied,in order to clarify the mechanism of kinetin delaying the senescence of cut foliage of Nephrolepis cordifoliaL..The results showed that after 12 days in vase,compared with control (deionized water,CK),the contents of hydrogen peroxide,superoxide anion and malondialdehyde and the electrical conductivity of cut leaves treated with KT10 were decreased by 92.06%,20.72%,410.34% and 104.40%,respectively,followed by KT7.5 (-46.46%,-18.09%,-173.39%,-59.93%) and KT5 (-23.69%,-13.40%,-63.58%,-37.39%).These results showed that KT could inhibit the production of reactive oxygen species and maintain the integrity and stability of cell membrane.KT10 increased the activities of superoxide dismutase (19.32%) and catalase (86.36%),while KT7.5 increased by 13.37% and 42.42%,and KT5 increased by 7.76% and 31.82%,respectively,and both of them reduced the toxic effect of reactive oxygen species.meanwhile,KT10 treatment also maintained the balance of AsA-GSH cycle by increasing the activities of ascorbate peroxidase (225.00%),glutathione reductase (366.67%) and dehydroascorbate reductase (566.67%),and decreasing the activity of total ascorbate (-442.12%),glutathione (-27.90%),alleviated oxidative damage.KT7.5 (-134.17%,-15.30%) and KT5 (-65.94%,-11.82%) also decreased the content of ascorbate and glutathione to varying degrees.In conclusion,kinetin could effectively retard leaf senescence by inhibiting reactive oxygen species content,maintaining membrane stability,activating antioxidant defense mechanism and balancing the AsA-GSH cycle,and the KT10 had the best anti-senescence effect,followed by KT7.5,and the KT5 had the lowest effect.

参考文献/References:

[1]LIU J,MA Z,SHI R,et al.Effects of nine kinds of household vase solutions on fresh-keeping effect of lilium cut flowers[J].Asian Agricultural Res,2021,13(2):35-40.[2]REN B,HU J,ZHANG J,et al.Effects of urea mixed with nitrapyrin on leaf photosynthetic and senescence characteristics of summer maize (Zea mays L.) waterlogged in the field[J].J Integr Agr,2020,19(6):1586-1595.[3]ASAD M A U,WANG F,YE Y,et al.Contribution of ABA metabolism and ROS generation to sugar starvation-induced senescence of rice leaves[J].Plant Growth Regul,2021,95(2):241-257.[4]LEGOCKA J,SZWEYKOWSKA A.Effect of kinetin on nucleic acid synthesis in senescing and young leaves in Brassica oleracea L.var. gongylodes L[J].Acta Soc Bot Pol,2015,44(4):553-565.[5]YALIKUNJIANG A,SHU H,ZHANG L,et al.Cytokinin regulates apical hook development via the coordinated actions of EIN3/EIL1 and PIF transcription factors in Arabidopsis[J].J Exp Bot,2021,73(1):213-227.[6]EISINGER W.Role of cytokinins in carnation flower senescence[J].Plant Physiol,1977,59(4):707-709.[7]SEEMA,KHOKHAR M,MUKHERJEE D.Role of kinetin and a morphactin in leaf disc senescence of Raphanus sativus L.under low light[J].Physiol Mol Biol Pla,2011,17(3):247-253.[8]TEERARAK M,LAOSINWATTANA C.Essential oil from ginger as a novel agent in delaying senescence of cut fronds of the fern (Davallia solida (G.Forst.)Sw.)[J].Postharvest Biol Tec,2019,156:110927.[9]ALI S,BANO A.Leaf and nodule senescence in chickpea (Cicer arietinum L.) and the role of plant growth regulators[J].Pak J Bot,2008,40(6):2481-2492.[10]SHAHS H.Kinetin improves photosynthetic and antioxidant responses of Nigella sativa to counteract salt stress[J].Russ J Plant Physl,2011,58(3):454-459.[11]ACIDRI R,SAWAI Y,SUGIMOTO Y,et al.Exogenous kinetin promotes the nonenzymatic antioxidant system and photosynthetic activity of coffee (Coffea arabica L.) plants under cold stress conditions[J].Plants,2020,9(2):281.[12]EDMOND G M,ALFONSO A,CRISTINA M,et al.Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.)[J].J Exp Bot,2008,59(11):3039-3050.[13]BAI T,LI C,MA F,et al.Responses of growth and antioxidant system to root-zone hypoxia stress in two Malus species[J].Plant Soil,2010,327(1/2):95-105.[14]XIA F,CHENG H,CHEN L,et al.Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds[J].Bmc Plant Biol,2020,20(1):104.[15]JORGE G,MARJORIE M R,RICARDO T,et al.Salicylic acid improves antioxidant defense system and photosynthetic performance in Aristotelia chilensis plants subjected to moderate drought stress[J].Plants,2022,11(5):639.[16]ZHANG F,WANG F,LOU Z,et al.Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza)[J].Chemosphere,2007,67(1):44-50.[17]JCCDS A,DMRS A,DJA B,et al.Glyphosate hormesis attenuates water deficit stress in safflower (Carthamus tinctorius L.) by modulating physiological and biochemical mediators[J].Sci Total Environ,2022,810:152204.[18]MONIKA K,ANETA H,PETRA M,et al.Multi-component antioxidative system and robust carbohydrate status,the essence of plant arsenic tolerance[J].Antioxidants,2020,9(4):283.[19]NAKANO Y,ASADA K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J].Plant Cell Physiol,1981,22:867-880.[20]HOSSAIN M A,NAKANO Y,ASADA K.Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide[J].Plant Cell Physiol,1984,25(3):385-395.[21]SHIGEOKA S,NAKANO Y,KITAOKA S.Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase[J].Biochem J,1980,186(1):377-380.[22]KHAN I,SINGH V,CHAUDHARY A K.Hepatoprotective activity of Pinus roxburghii Sarg.wood oil against carbon tetrachloride and ethanol induced hepatotoxicity[J].Bangl J Pharmacol,2012,7(2):94-99.[23]NAGALAKSHMI N,PRASAD M N V.Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus hijugatus[J].Plant Sci,2001,160:291-299.[24]WU Y,HU L,LIAO W,et al.Foliar application of 5-aminolevulinic acid (ALA) alleviates NaCl stress in cucumber (Cucumis sativus L.) seedlings through the enhancement of ascorbate-glutathione cycle[J].Sci Hortic-Amsterdam,2019,257(C):108761.[25]KIYOSHI T,YOSHIKO S,NORIAKI K,et al.O3 Tolerance and the ascorbate-dependent H2O2 decomposing system in chloroplasts[J].Plant Cell Physiol,1985,26(7):1425-1431.[26]ZHANG Q,ZHAI J,WEI Y,et al.Effects of shading on the senescence and photosynthetic physiology of the early-flowering rice mutant FTL10 at noon[J].J Plant Growth Regul,2020,39(7):1-9.[27]NAGANO T,YAMAO S,TERACHI A,et al.D-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species[J].Life Sci Alliance,2019,2(1):e201800045.[28]JAJIC I,SARNA T,STRZALKA K,et al.Senescence,stress,and reactive oxygen species[J].Plants,2015,4(3):393-411.[29]NOCTOR G,FOYER C H.Ascorbate and glutathione：Keeping active oxygen under control[J].Annu Rev Plant Phys,1998,49:249-279.[30]DE M,VIEIRA M,SIMES A,et al.Physiological effect of kinetin on the photosynthetic apparatus and antioxidant enzymes activities during production of anthurium[J].Hortic Plant J,2018,4(5):182-192.[31]COSTA J H,ROQUE A L M,SHAHID A,et al.Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events and specificity of gene members linked to development and stress conditions[J].International Journal of Biological Macromolecules,2021,187:528-543.[32]WANG H,CUI S,WU D,et al.Effects of kinetin on arsenic speciation and antioxidative enzymes in fronds of the arsenic hyperaccumulator Pteris cretica var.nervosa and non-hyperaccumulator Pteris ensiformis[J].Environmental and Experimental Botany,2021,191(1):104622.[33]BALI A S,SIDHU G.Arsenic acquisition,toxicity and tolerance in plants-from physiology to remediation：A review[J].Chemosphere,2021,283:131050.[34]SANTWANA T,MOHAN P S.Exogenous application of phytohormones modulates pesticide-induced injury in two rice field cyanobacteria：Toxicity alleviation by up-regulation of ascorbate-glutathione cycle[J].Plant Stress,2021(3):100046.[35]CHEN J,LI F,LI Y,et al.Exogenous procyanidin treatment delays senescence of harvested banana fruit by enhancing antioxidant responses and in vivo procyanidin content[J].Postharvest Biol Tec,2019,158(C):110999.[36]FAN J,CHEN K,XU J,et al.Physiological effects induced by aluminium and fluoride stress in tall fescue (Festuca arundinacea Schreb)[J].Ecotox Environ Safe,2022,231:113192.[37]YU K,WEI J,MA Q,et al.Senescence of aerial parts is impeded by exogenous gibberellic acid in herbaceous perennial Paris polyphylla[J].J Plant Physiol,2009,166(8):819-830.[38]AHANGER M A,MIR R A,ALYEMENI M N,et al.Combined effects of brassinosteroid and kinetin mitigates salinity stress in tomato through the modulation of antioxidant and osmolyte metabolism[J].Plant Physiol Bioch,2020,147(C):31-42.

相似文献/References:

[1]刘旭,刘博,吕春华,等.不同激素处理对非洲菊开花的影响[J].北方园艺,2012,36(24):83.
　LIU Xu,LIU Bo,LV Chun-hua,et al.Effect of Different Hormone Treatments on Flowering of Gerbera[J].Northern Horticulture,2012,36(07):83.
[2]左进城,李秀玲,张鹏,等.吲哚乙酸和激动素对苦草种子萌发和幼苗生长的影响[J].北方园艺,2014,38(23):53.
　ZUO Jin-cheng,LI Xiu-ling,ZHANG Peng,et al.Effect of IAA and KT on Seed Germination and Seedling Growth of Vallisneria spiralis[J].Northern Horticulture,2014,38(07):53.
[3]曹慧颖,张立军,骆祯,等.番茄活体再生中反式玉米素含量的变化[J].北方园艺,2015,39(24):66.[doi:10.11937/bfyy.201524020]
　CAO Huiying,ZHANG Lijun,LUO Zhen,et al.Change of Trans-zeatin in Tomato Regeneration in vivo[J].Northern Horticulture,2015,39(07):66.[doi:10.11937/bfyy.201524020]
[4]鲁彦君.木榄愈伤组织诱导影响因子研究[J].北方园艺,2016,40(15):92.[doi:10.11937/bfyy.201615023]
　LU Yanjun.Study on Factors Affecting Callus Initiation From Bruguiera gymnorrhiza (L.) Savigny[J].Northern Horticulture,2016,40(07):92.[doi:10.11937/bfyy.201615023]
[5]胡燕梅,蒋光玉,方中明.椒样薄荷叶片愈伤组织诱导与抗褐变[J].北方园艺,2018,42(17):145.[doi:10.11937/bfyy.20172146]
　HU Yanmei,JIANG Guangyu,FANG Zhongming.Induction and Anti-browning of Leaf Callus From Mentha piperita L.[J].Northern Horticulture,2018,42(07):145.[doi:10.11937/bfyy.20172146]
[6]钟灿,劳嘉,金剑,等.基于激素及其配比探讨多花黄精种子组培体系的建立[J].北方园艺,2020,44(13):118.[doi:10.11937/bfyy.20194082]
　ZHONG Can,LAO Jia,JIN Jian,et al.Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua[J].Northern Horticulture,2020,44(07):118.[doi:10.11937/bfyy.20194082]
[7]万珠珠,谭秀梅,刘敏,等.细胞分裂素处理对香石竹切花保鲜效果的影响[J].北方园艺,2020,44(21):85.[doi:10.11937/bfyy.20200092]
　WAN Zhuzhu,TAN Xiumei,LIU Min,et al.Effects of Cytokinins on Preservation Effect Carnation Cut Flower[J].Northern Horticulture,2020,44(07):85.[doi:10.11937/bfyy.20200092]
[8]马玮超,刘文静,孙玉镯.外源细胞分裂素对香菇生长和品质的影响[J].北方园艺,2021,(17):131.[doi:10.11937/bfyy.20203124]
　MA Weichao,LIU Wenjing,SUN Yuzhuo.Effects of Exogenous Cytokinin on the Growth and Quality of Lentinus edodes[J].Northern Horticulture,2021,(07):131.[doi:10.11937/bfyy.20203124]
[9]魏晓琼,贾文飞,马靖恒,等.植物生长调节剂对植株生长发育的影响概述[J].北方园艺,2022,(04):118.[doi:10.11937/bfyy.20213491]
　WEI Xiaoqiong,JIA Wenfei,MA Jingheng,et al.Review on the Effects of Plant Growth Regulators on Plant Growth and Development[J].Northern Horticulture,2022,(07):118.[doi:10.11937/bfyy.20213491]

备注/Memo

第一作者简介：孙彩艺（1998-），女，硕士研究生，研究方向为园林植物生理生态学。E-mail：2501941997@qq.com.责任作者：张璐（1982-），男，博士，教授，现主要从事园林植物生理生态学等研究工作。E-mail：caszhanglu@hotmail.com.基金项目：浙江农林大学人才启动资助项目(2021FR041)。收稿日期：2022-09-03

更新日期/Last Update: 2023-05-04