LIU Ye,MO Shilun,XIANG Liqing,et al.Salt Tolerance Evaluation of Different Germplasms of Andrographis paniculata During Germinating[J].Northern Horticulture,2023,(04):101-110.[doi:10.11937/bfyy.20221653]
不同种质穿心莲萌发期耐盐性评价
- Title:
- Salt Tolerance Evaluation of Different Germplasms of Andrographis paniculata During Germinating
- Keywords:
- Andrographis paniculata; seed germination; salt tolerance
- 文献标志码:
- A
- 摘要:
- 以10份不同产地来源的穿心莲种子为试材，采用培养皿纸上发芽法模拟盐胁迫环境，研究了NaCl胁迫对不同种质穿心莲萌发特性的影响，结合隶属函数法和系统聚类分析法对其耐盐性进行综合评价，以期为穿心莲耐盐种质筛选、种植和推广提供参考依据。结果表明：盐胁迫对各穿心莲种质种子的发芽率、发芽势、相对盐害率、可溶性蛋白质含量、过氧化氢酶（CAT）活性、脯氨酸（Pro）含量、种子活力均有显著影响，但不同种质之间响应情况不同；穿心莲在萌发期表现出一定耐盐性，不同种质穿心莲之间的耐盐性强弱不同，耐盐性综合评价将其分成了耐盐性较强、耐盐性中等、敏感3种类型,种质A（广西玉林）属于强耐盐；种质I（福建漳州）、种质E（广东湛江常规种）等属于中耐盐；种质F（广东湛江大叶种）属于盐敏感。
- Abstract:
- Ten Andrographis paniculata seeds of different origins were used as experimental materials，and the effects of NaCl stress on the germination characteristics of different germplasms of Andrographis paniculata were studied by simulating the salt stress environment using the method of germination on dish paper，and their salt tolerance was comprehensively evaluated by combining the affiliation function method and systematic cluster analysis，in order to provide reference for the screening，planting and promotion.The results showed that salt stress had significant effects on the seed germination rate，germination potential，relative salt damge rate，soluble protein content，catalase (CAT) activity，proline (Pro) content，and seed vigor of different germplasms of Andrographis paniculata，but different germplasms responded differently.Andrographis paniculata exhibited salt tolerance during the germination period.The comprehensive evaluation of salt tolerance classified them into three types,strongly salt-tolerant，moderately salt-tolerant，and sensitive.Germplasm A (Yulin，Guangxi) being strongly salt-tolerant；germplasm I (Zhangzhou，Fujian)，E (Zhanjiang conventional species，Guangdong) et al.being moderately salt-tolerant；germplasm F (Zhanjiang，Guangdong，large-leaved species) being sensitive.
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备注/Memo
第一作者简介：刘叶（1996-），女，硕士研究生，研究方向为中药资源开发及品质评价。E-mail:m18797011789@163.com.责任作者：邵艳华（1987-），女，博士，讲师，现主要从事中药资源开发及品质评价等研究工作。E-mail：shaoyh_gz@126.com.基金项目：广东省重点领域研发计划“现代种业”重大专项资助项目（2020B20221002）。收稿日期：2022-04-22