[1]李明,曹慧,段高飞,等.葡萄VvLBD28基因的基本特征和表达分析[J].北方园艺,2022,(17):57-63.[doi:10.11937/bfyy.20220480]
　LI Ming,CAO Hui,DUAN Gaofei,et al.Characteristics and Expression Analysis of VvLBD28 Gene in Grape[J].Northern Horticulture,2022,(17):57-63.[doi:10.11937/bfyy.20220480]

点击复制

葡萄VvLBD28基因的基本特征和表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年17 页码: 57-63 栏目: 出版日期: 2022-09-15

Title:: Characteristics and Expression Analysis of VvLBD28 Gene in Grape

作者:: 李明1; 曹慧1; 段高飞1; 李高青2; 许瑞瑞1; （1.潍坊学院生物与农业工程学院，山东省高等学校生物化学与分子生物学重点实验室，山东潍坊 261061；2.寿光市农业局，山东潍坊 262700）

Author(s):: LI Ming1; CAO Hui1; DUAN Gaofei1; LI Gaoqing2; XU Ruirui1; (1.College of Biological and Agricultural Engineering,Weifang University/Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong,Weifang,Shandong 261061；2.Agricultural Bureau of Shouguang,Weifang,Shandong 262700)

关键词:: 葡萄; VvLBD28基因; 胁迫; 表达分析

Keywords:: Vitis vinifera; VvLBD28 gene; stress; expression analysis

DOI:: 10.11937/bfyy.20220480

文献标志码:: A

摘要:: 以葡萄为试材，采用生物信息学分析和实时荧光定量PCR方法，研究了VvLBD28基因的特征、组织表达模式及干旱、盐和温度胁迫对VvLBD28表达情况的影响，以期为研究该基因参与的胁迫响应过程提供参考依据。结果表明：葡萄VvLBD28包含Leu拉链模块，属于第一类LBD基因亚家族，与苹果、水蜜桃以及模式植物拟南芥和烟草等的LBD蛋白均有较高的同源性；VvLBD28在葡萄茎中的表达量最高，稍高于叶片中，在根中的表达量最低；盐处理和39 ℃高温处理后，VvLBD28的表达量变化不明显，而干旱和低温4 ℃均能使VvLBD28的表达量明显下调。

Abstract:: Taking grape as test material,the characteristics of VvLBD28 gene,tissue expression pattern and the effects of drought,salt and temperature stress on VvLBD28 expression were studied by bioinformatics analysis and real-time fluorescent quantitative PCR,in order to provide reference for the study of the stress response process involved in this gene.The results showed that VvLBD28 contained complete leu zipper,belonging to Class I subfamily,highly homologous and high similarity with apple,peach,Arabidopsis and tobacco LBD genes.qRT-PCR analysis showed that the expression level of VvLBD28 was the highest in stem,slightly higher than that in leaf,and the lowest in root of grape.The expression level of VvLBD28 did not change significantly under salt stress and 39 ℃,but decreased significantly under mannitol and 4 ℃,which means VvLBD28 was down-regulated by drought and cold stresses.

参考文献/References:

[1]卢寰,时振英.高等植物特有的LBD基因的分子生物学功能研究进展[J].植物生理学报,2013,49(9)：833-846.[2]石玉,沈诗雅,张倩茹,等.LBD基因家族研究进展[J].中国细胞生物学学报,2019,41(4)：738-745.[3]SHUAI B,REYNAGA-PEA C G,SPRINGER P S.The lateral organ boundaries gene defines a novel,plant-specific gene family[J].Plant Physiology,2002,129(2)：747-761.[4]YANG Y,YU X,WU P.Comparison and evolution analysis of two rice subspecies LATERAL ORGAN BOUNDARIES domain gene family and their evolutionary characterization from Arabidopsis[J].Molecular Phylogenetics and Evolution,2006,39：248-262.[5]SCHNABLE P S,WARE D,FULTON R S,et al.The B73 maize genome：Complexity,diversity,and dynamics[J].Science,2009,326：1112-1115.[6]MANGEON A,BELL E M,LIN W,et al.Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis[J].Journal of Experimental Botany,2011,62(1)：221-233.[7]王小非,刘鑫,苏玲,等.番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析[J].中国农业科学,2013,46(12)：2501-2513.[8]WANG X,ZHANG S,SU L,et al.A genome-wide analysis of the LBD (lateral organ boundaries domain) gene family in Malus domestica with a functional characterization of MdLBD11[J].PLoS One,2013,8(2)：e57044.[9]CAO H,LIU C Y,LIU C X,et al.Genomewide analysis of the lateral organ boundaries domain gene family in Vitis vinifera[J].Journal of Genetics,2016,95(3)：1-12.[10]GUO B J,WANG J,LIN S,et al.A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)[J].Journal of Zhejiang University Science B (Biomed & Biotechnol),2016,17(10)：763-774.[11]YANG T,FANG G Y,HE H,et al.Genome-wide identification,evolutionary analysis and expression profiles of LATERAL ORGAN BOUNDARIES DOMAIN gene family in lotus japonicus and medicago truncatula[J].PLoS One,2016,11(8)：e0161901.[12]郑忠凡,张亚利,胡灿,等.辣椒全基因组中LBD转录因子的鉴定与表达分析[J].园艺学报,2016,43(4)：683-694.[13]YU Q,HU S M,DU J C,et al.Genome-wide identification and characterization of the lateral organ boundaries domain gene family in Brassica rapa var. rapa[J].Plant Diversity,2019,42(1):52-60.[14]XU C,LUO F,HOCHHOLDINGER F.LOB domain proteins：Beyond lateral organ boundaries[J].Trends in Plant Science,2016,21(2)：159-67.[15]XU C,CAO H,ZHANG Q,et al.Control of auxin-induced callus formation by bZIP59-LBD complex in Arabidopsis regeneration[J].Nature Plants,2018(4):108-115.[16]LIU S,WANG B,LI X,et al.Lateral organ boundaries domain 19 (LBD19) negative regulate callus formation in Arabidopsis[J].Plant Cell Tissue and Organ Culture,2019,137：485-494.[17]XU B,LI Z,ZHU Y,et al.Arabidopsis genes AS1,AS2,and JAG negatively regulate boundary-specifying genes to promote sepal and petal development[J].Plant Physiology,2008,146(2)：566-575.[18]JUN J H,HA C M,FLETCHER J C.BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in Arabidopsis by directly activating ASYMMETRIC LEAVES2[J].The Plant Cell,2010,22(1)：62-76.[19]ZHOU L L,SHI M Z,XIE D Y.Regulation of anthocyanin biosynthesis by nitrogen in TTG1-GL3/TT8-PAP1-programmed red cells of Arabidopsis thaliana[J].Planta,2012,236(3)：825-837.[20]SHENG L,HU X,DU Y,et al.Non-canonical WOX11-mediated root branching contributes to plasticity in Arabidopsis root system architecture[J].Development,2017,144(17)：3126-3133.[21]HU Y,ZHANG J,JIA H,et al.Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(4)：521-529.[22]LIU Y,WANG H,JIANG Z,et al.Genomic basis of geographical adaptation to soil nitrogen in rice[J].Nature,2021,590:600-605.[23]郭兆来.拟南芥AtLBD15调控植物干旱胁迫的分子机理研究[D].昆明：昆明理工大学,2019.[24]ZOU X,DU M,LIU Y,et al.CsLOB1 regulates susceptibility to citrus canker through promoting cell proliferation in citrus[J].The Plant Journal,2021,106(4):1039-1057.[25]GRIMPLET J,PIMENTEL D,AGUDELO-ROMERO P,et al.The LATERAL ORGAN BOUNDARIES domain gene family in grapevine：Genome-wide characterization and expression analysis during developmental processes and stress responses[J].Scientific Reports,2017（7）：15968.[26]何红红,马宗恒,张元霞,等.葡萄LBD基因家族的鉴定与表达分析[J].中国农业科学,2018,51(21)：4102-4118.[27]MAJER C,HOCHHOLDINGER F.Defifi ning the boundaries：Structure and function of LOB domain proteins[J].Trends in Plant Science,2011,16(1)：47-52.

相似文献/References:

[1]单守明,平吉成,刘晶,等.6-BA对染卷叶病毒葡萄光合作用和果实品质的影响[J].北方园艺,2013,37(04):5.
[2]梁浩.枣庄地区气候因子对葡萄生产模式选择的影响[J].北方园艺,2013,37(04):55.
[3]张广和,于晓丽,栾炳辉,等.倍创与杀菌剂混用对葡萄果实病害的防效评价[J].北方园艺,2013,37(04):127.
[4]李海峰,王瑞华,韩琛,等.新疆葡萄平衡施肥技术发展现状及展望[J].北方园艺,2013,37(04):185.
[5]史洪琴,蒋丽光.中短剪葡萄结实特性比较[J].北方园艺,2014,38(12):34.
　SHI Hong-qin,JIANG Li-guang.Comparison of Grape Fruit Characteristics After Moderate and Short Pruning[J].Northern Horticulture,2014,38(17):34.
[6]张剑侠,吴行昶,杨亚州.葡萄种间杂种的抗寒性评价[J].北方园艺,2014,38(13):1.
　ZHANG Jian-xia,WU Xing-chang,YANG Ya-zhou.Cold-resistance Evaluation in Grape Interspecific Hybrids[J].Northern Horticulture,2014,38(17):1.
[7]商佳胤,田淑芬,集贤,等.果袋颜色对设施葡萄果实品质的影响[J].北方园艺,2014,38(11):42.
　SHANG Jia-yin,TIAN Shu-fen,JI Xian,et al.Effect of Fruit Bag Color on Grape Quality in Facility Condition[J].Northern Horticulture,2014,38(17):42.
[8]王文平.肃州区非耕地日光温室葡萄反季节高效节水栽培技术[J].北方园艺,2014,38(10):43.
　[J].Northern Horticulture,2014,38(17):43.
[9]周恒,饶鹏,田如英,等.不同栽培方式下五个葡萄品种在贵州铜仁地区的生产表现[J].北方园艺,2014,38(14):22.
　ZHOU Heng,RAO Peng,TIAN Ru-ying,et al.Five Grape Varieties Under Different Cultivations in the Production Performance in Guizhou Tongren Area[J].Northern Horticulture,2014,38(17):22.
[10]吴久赟,郭峰,杨承时,等.不同叶果比对葡萄果实生长的影响[J].北方园艺,2014,38(04):33.
　WU Jiu-yun,GUO Feng,YANG Cheng-shi,et al.Study on the Influence of Different Leaf-fruit Ratio on Growth of Grape[J].Northern Horticulture,2014,38(17):33.

备注/Memo

第一作者简介：李明（1982-），男，博士，讲师，现主要从事果树学等研究工作。E-mail:lm11a@163.com.责任作者：许瑞瑞（1982-），女，博士，教授，现主要从事作物生物学基因表达调控等研究工作。E-mail:xuruirui2006@163.com.基金项目：国家自然科学基金面上资助项目（32072518）；作物生物学国家重点实验室开放课题资助项目（2021KF06）。收稿日期：2022-02-12

更新日期/Last Update: 2022-11-22