GAO Xu,TAN Guoyin,ZHU Changzhi,et al.Genetic Diversity of F1 Hybrids Population of Different Broccoli Varieties[J].Northern Horticulture,2022,(17):1-8.[doi:10.11937/bfyy.20220454]
不同青花菜品种F1群体的遗传多样性分析
- Title:
- Genetic Diversity of F1 Hybrids Population of Different Broccoli Varieties
- Keywords:
- broccoli; SSR markers; genetic diversity; genetic distance; gene flow
- 文献标志码:
- A
- 摘要:
- 以7个不同青花菜品种的各144份F1群体单株为试材，采用SSR分子标记方法，研究了青花菜杂交F1代的遗传多样性及变异规律，以期为青花菜新品种选育提供参考依据。结果表明：3对SSR引物在供试材料中共检测到了27个等位基因位点，平均每个SSR标记9个等位基因；有效等位基因数（Ne）、Shannon′s 多样性指数（I）、观测杂合度（Ho）、期望杂合度（He）、Nei′s期望杂合度和平均杂合度的平均值分别为2.536 4、1.129 7、0.768 0、0.598 7、0.598 4和0.454 8，遗传分化系数表明其变异主要存在于不同F1子代群体内（74.07%）；分子方差分析表明青花菜杂交F1代遗传变异主要来源于其群体内（82.12%），这与遗传分化系数结果一致；UPGMA聚类分析结果表明，在遗传距离为0.2处可将7个不同青花菜品种分为3个类群；PCA分析结果表明，同个青花菜品种F1子代或遗传距离相近的青花菜品种能够聚类到一起，这与群体遗传结构分析结果基本一致，而且不同青花菜品种的F1子代有交叉重叠现象，同时也说明它们之间存在着广泛的基因交流。
- Abstract:
- Taking 144 F1 populations of 7 different broccoli varieties as test materials,SSR molecular marker method was adopted to research genetic diversity and variation of F1 generation of broccoli crosses,in order to provide reference for breeding of new broccoli varieties.The results showed that a total of 27 alleles were detected by 3 pairs of SSR primers in the tested materials,and the average number of alleles was 9 per SSR marker；the average value of the number of effective alleles（Ne），Shannon′s diversity index（I），observed heterozygosity（Ho），expected heterozygosity（He），Nei′s expected heterozygosity and average heterozygosity were 2.536 4，1.129 7，0.768 0，0.598 7，0.598 4 and 0.454 8，respectively；the genetic differentiation coefficient indicated that genetic variation mainly existed in different F1 progeny populations (74.07%)；the analysis of molecular variance showed that the genetic variation of broccoli F1 generation mainly came from within its population (82.12%),which was consistent with the result of genetic differentiation coefficient；the results of UPGMA cluster analysis showed that 7 different broccoli varieties could be clustered into 3 groups at the genetic distance of 0.2；F1 progenies of the same broccoli variety or broccoli varieties with similar genetic distance could be clustered together by PCA analysis，which was basically consistent with the results of population genetic structure analysis，and F1 offspring of different broccoli varieties had the phenomenon of cross overlap,which also existed in extensive gene exchange among them.
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备注/Memo
以7个不同青花菜品种的各144份F1群体单株为试材，采用SSR分子标记方法，研究了青花菜杂交F1代的遗传多样性及变异规律，以期为青花菜新品种选育提供参考依据。结果表明：3对SSR引物在供试材料中共检测到了27个等位基因位点，平均每个SSR标记9个等位基因；有效等位基因数（Ne）、Shannon′s 多样性指数（I）、观测杂合度（Ho）、期望杂合度（He）、Nei′s期望杂合度和平均杂合度的平均值分别为2.536 4、1.129 7、0.768 0、0.598 7、0.598 4和0.454 8，遗传分化系数表明其变异主要存在于不同F1子代群体内（74.07%）；分子方差分析表明青花菜杂交F1代遗传变异主要来源于其群体内（82.12%），这与遗传分化系数结果一致；UPGMA聚类分析结果表明，在遗传距离为0.2处可将7个不同青花菜品种分为3个类群；PCA分析结果表明，同个青花菜品种F1子代或遗传距离相近的青花菜品种能够聚类到一起，这与群体遗传结构分析结果基本一致，而且不同青花菜品种的F1子代有交叉重叠现象，同时也说明它们之间存在着广泛的基因交流。