AbulimitiYUMAIER,KorbanjhonBRAD.Determination of Total Alkaloids in Aconitum leucostomum by UV-vis Spectrophotometry[J].Northern Horticulture,2022,(12):94-98.[doi:10.11937/bfyy.20214481]
紫外可见分光光度法测定白喉乌头中总生物碱的含量
- Title:
- Determination of Total Alkaloids in Aconitum leucostomum by UV-vis Spectrophotometry
- Keywords:
- Aconitum leucostomum; total alkaloids; spectrophotometry; ultrasonic extraction; content determination
- 文献标志码:
- A
- 摘要:
- 以白喉乌头不同部位为试材，采用无水乙醇超声波法提取乌头碱，利用分光光度计于231 nm波长条件下测定吸收度，计算总生物碱的含量，从而建立不同产地白喉乌头不同部位总生物碱的含量测定方法，以期为乌头的开发利用提供参考依据。结果表明：乌头碱在0.000 4～0.002 0 mg?L-1浓度范围内线性关系良好，回归方程Y=0.179 5X+0.511 72，r=0.999 4，精密度良好，RSD=3.2%（n=3）；样品溶液显色2 h内稳定，RSD=2.14%(n=3)，测定的重现性良好，RSD=1.49%(n=3)；平均加样回收率为99.886%，RSD=1.27%(n=3)，平均含量0.063 7 mg?g-1，RSD=0.716%（n=3）。
- Abstract:
- Different parts of Aconitum leucostomum were used as test materials and extracted by anhydrous ethanol ultrasonic method,the absorbance was measured with a spectrophotometer at a wavelength of 231 nm,and the total alkaloids were calculated.A method for the determination of total alkaloids in different parts of Aconitum leucostomum from different origins was established,in order to provide reference for the development and utilization of Aconitum carmichaeli.The results showed that aconitine had a good linear relationship in the concentration range of 0.000 4-0.002 0 mg?L-1,the regression equation was Y=0.179 5X+0.511 72,r= 0.999 4,the precision was good,RSD=3.2% (n=3).The sample solution was stable within 2 hours fter color development,RSD=2.14% (n=3).The reproducibility of this method was good,RSD=1.49% (n=3).The average recovery was 99.886%,RSD=1.27% (n=3),the average content was 0.063 7 mg?g-1,RSD=0.716% (n=3).
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备注/Memo
第一作者简介：阿不力米提?玉麦尔(1978-)，男，维吾尔族，硕士，讲师，现主要从事中药与天然药物等研究工作。E-mail:ablimit0608@163.com.责任作者：库尔班江?巴拉提(1962-)，男，维吾尔族，本科，教授，现主要从事中药与天然药物等研究工作。E-mail:korbanjhon@126.com.收稿日期：2021-11-05