YIN Dongmei,WANG Yueyue,DONG Tingting,et al.Influence of Ethylene Response Factor PhERF2 on Waterlogging Resistance of Petunias[J].Northern Horticulture,2021,(22):82-90.[doi:10.11937/bfyy.20212059]
乙烯响应因子PhERF2对矮牵牛耐涝性的影响
- Title:
- Influence of Ethylene Response Factor PhERF2 on Waterlogging Resistance of Petunias
- Keywords:
- petunias; waterlogging stress; ethylene responsive factor; antioxidant enzymes; anaerobic respiration enzyme
- 文献标志码:
- A
- 摘要:
- 以矮牵牛7个株系（乙烯响应因子PhERF2过表达株系C、D、I，RNAi-PhERF2沉默株系1A、1B、4B及其野生型(WT)）为试材，比较矮牵牛PhERF2基因的过表达株系、沉默株系及其野生型在涝渍环境中的表型，测定抗氧化酶活性和无氧呼吸酶活性，以探讨乙烯响应因子PhERF2对矮牵牛耐涝性的影响。结果表明：涝渍处理72 h，C、D、I的丙二醛（MDA）含量仅为野生型的33.33%、45.1%和58.5%，而超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性总体高于沉默株系与野生型，较高的抗氧化酶活性有利于增强植株的抗涝能力，表明过表达株系通过提高抗氧化酶活性、降低MDA含量减少膜脂过氧化损伤以适应涝渍胁迫；7个株系根系的乙醇脱氢酶（ADH）、乳酸多氢酶（LDH）和丙酮酸脱羟酶（PDC）活性均呈先上升后下降趋势，其中C、D、I的LDH活性整体上显著低于沉默株系与野生型，在涝渍处理72 h时三者的LDH含量分别为0 h的51.3%、78.0%、58.8%。涝渍处理4 d时，4B的涝害表型症状最为严重，所有叶片萎蔫下垂并黄化干枯。综上所述，沉默株系的涝害表型症状最为严重，过表达株系的抗氧化酶活性与无氧呼吸酶活性总体高于野生型与沉默株系，表明过表达株系可能具有更强的清除活性氧的能力，并且更具减轻乳酸、乙醇等代谢产物对细胞的毒害作用的能力，以缓解植物在逆境下所受的伤害，说明其对涝渍胁迫具有更强的耐受力。该研究结果初步证明了乙烯响应因子PhERF2可正向调控植株的耐涝性。
- Abstract:
- Taking 7 petunia lines (ethylene response factor PhERF2-overexpressed lines C,D,I,RNAi-PhERF2 silent lines 1A,1B,4B and their wild-type (WT) )as materials,the phenotypes of overexpression lines,silent lines and wild-type of Petunia hybrid PhERF2 gene in waterlogging environment were compared, and the activities of antioxidant enzymes and anaerobic respiratory enzymes were measured to explore the effect of ethylene response factor PhERF2 on waterlogging tolerance of Petunia hybrid.The results showed that,after 72 hours waterlogging treatment,the malondialdehyde(MDA) contents of C,D and I were only 33.33%,45.10% and 58.50% of those of the wild type,while the activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) were generally higher than those of the silent type and the wild type.Higher antioxidant enzyme activity was beneficial to enhance waterlogging resistance of plants.The results showed that the overexpressed lines could adapt to waterlogging stress by increasing antioxidant enzyme activity and reducing MDA content.The activity of dehydrogenase (ADH),lactate dehydrogenase (LDH) and pyruvate decarboxylase (PDC) in the roots of 7 lines showed a trend of first increasing and then decreasing,among which the LDH activity of C,D and I was significantly lower than that of the silenced lines and wild-type lines on the whole.The LDH content of the three lines was 51.3%,78.0% and 58.8% of 0 hours respectively at 72 hours after waterlogging treatment,the increasing of anaerobic respiration enzyme activity could promote the adaptation of plant roots to hypoxic environment.In the 4-day waterlogging treatment,the phenotypic symptoms of waterlogging damage at 4B were the most serious,and all the leaves were wilted,saggy and yellowed and dried.Combined with the above results,the phenotypic symptoms of waterlogging were the most serious in silenced lines,the antioxidant enzyme activity and anaerobic respiration enzyme activity of over expressed lines were higher than those of wild-type and silenced lines.It indicated that overexpressed lines may have a stronger ability to clear reactive oxygen species and reduce the toxic effects of metabolites such as lactic acid and ethanol on cells,in order to alleviate the damage of plants under adversity,it shows that plants have stronger tolerance to water logging stress.The results of this study preliminarily demonstrate that ethylene response factor PhERF2 positively regulates plant waterlogging tolerance.
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备注/Memo
第一作者简介：尹冬梅（1983-），女，博士，副教授，硕士生导师，现主要从事园艺、植物生理与分子生物学等研究工作。E-mail: yindm@sit.edu.cn.责任作者：杨再强（1967-），男，博士，教授，博士生导师，现主要从事园艺与农业基础科学等研究工作。E-mail:yzq@nuist.edu.cn.基金项目：国家自然科学基金资助项目（31701963,41775104,41975142）；上海市科技兴农资助项目（2021-02-08-00-12-F00756）。收稿日期：2021-05-14