WEN Yonghui,CHEN Shuangxiu,WANG Qiong,et al.Establishment of Rapid Propagation Technology for Impatiens rubrostriata in vitro[J].Northern Horticulture,2021,(15):57-64.[doi:10.11937/bfyy.20204302]
红纹凤仙花离体快繁技术体系的建立
- Title:
- Establishment of Rapid Propagation Technology for Impatiens rubrostriata in vitro
- Keywords:
- Impatiens rubrostriata; primary culture; proliferation culture; rooting culture; sterilization method; cefotaxime sodium
- 文献标志码:
- A
- 摘要:
- 以红纹凤仙花（Impatiens rubrostriata Hook.f.）带芽茎段为试材，采用不同灭菌剂对外植体进行处理，通过添加不同种类植物生长调节剂及浓度的培养基对其进行初代培养、增殖及生根培养，从而筛选红纹凤仙花最佳灭菌方法、最佳诱导、增殖及生根培养基，以期为野生凤仙花属植物资源的引种驯化及开发利用奠定良好的技术基础。结果表明：红纹凤仙花带芽茎段的最适灭菌方法为75%酒精10 s+0.1%氯化汞6 min，成活率为68%；初代培养基中添加20 mg·L-1头孢噻肟钠时抑菌效果较好，成活率100%；最适初代培养基为MS+1.0 mg·L-16-BA+0.3 mg·L-1 NAA，苗壮且生长快；最适增殖培养基为MS+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA+0.010 mg·L-1 TDZ，增殖系数为6.65，苗生长快；最适生根培养基为MS+0.10 mg·L-1IBA，生根率100%。
- Abstract:
- The budding stems of Impatiens rubrostriata were used as materials.The explants were treated with different sterilants，and primary culture，proliferation and rooting culture.They were performed on the medium with different types of hormoneand concentrations to screen the best sterilization method，induction，proliferation and rooting medium of Impatiens rubrostriata.The results showed that the optimal sterilization method for Impatiens rubrostriatais 75% alcohol，10 seconds+0.1% mercury chloride for 6 minutes，the survival rate was 100%.When 20 mg·L-1cefotaxime sodium was added to the primary medium，the bacterial effect was better，the survival rate was 100%.The most suitable primary medium was MS+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA，under this circumstances，the seedlings were strong and the growth was fast.The optimal proliferation medium was MS+1.0 mg·L-1 6-BA+0.3 mg·L-1NAA+0.010 mg·L-1TDZ，the proliferation coefficient was 6.65，the seedlings grew fast.The most suitable rooting medium was MS+0.10 mg·L-1 IBA，and the rooting rate was 100%.The in vitro rapid propagation system for Impatiens rubrostriata was established，which will lay a good technical foundation for development and utilization of Impatiens rubrostriata.
参考文献/References:
[1]蔡秀珍,易任远,庄远红,等.凤仙花属植物种皮微形态特征在系统分类中的应用[J].园艺学报,2013,40(7):1337-1348.[2]CONG Y Y，XIANG Y L，LIU K M.Impatiens quadriloba sp.nov.(Balsaminaceae) from Sichuan，China[J].Nordic Journal of Botany，2010,28(3):309-312.[3]陈艺林.国产凤仙花属植物的研究[J].植物分类学报,1978,16(2):36-55.[4]相银龙.川西南凤仙花属Impatiens L.植物区系及亲缘关系研究[D].长沙:湖南师范大学,2011.[5]徐奇伟.凤仙花外敷治疗灰指甲50例[J].中国社区医师(医学专业),2010,12(8):123.[6]曾蕾.凤仙花属(Impatiens)棒凤仙亚属(Clavicarpa)的系统学研究[D].临汾:山西师范大学,2016.[7]赵贞.凤仙花愈伤组织诱导及其分化的研究[D].新乡:河南师范大学,2013.[8]王雪娟,程新利,柴瑞娟.睫毛萼凤仙花的组织培养及其愈伤组织的超微观察[J].广西植物,2011,31(2):262-265.[9]王越,刘燕.大旗瓣凤仙花的组织培养与快速繁殖[J].植物生理学通讯,2008,44(3):510.[10]SZEWCZYK K，SEZAI C S，ZIDORN C，et al.Phenolic constituents of the aerialparts of Impatiens glandulifera Royle (Balsaminaceae) and their antioxidant activities[J].Natural Product Research，2019，33(19):2851-2855.[11]ZHENG Y S，HU Y X，LIU K X，et al..Therapeutic effect of Impatiens balsamina, Lawsonia inermis L.and Henna on androgenetic alopecia in mice[J].J Southern Med Univ，2019，39(11):1376-1380.[12]中国科学院昆明植物研究所.云南植物志(第16卷)[M].北京:科学出版社,2006.[13]于胜祥.中国凤仙花[M].北京:北京大学出版社,2012.[14]SONG Y，YUAN M Y，PHILIPPE K.Chromosomal evolution in Balsaminaceae，with cytological observations on 45 species from Southeast Asia[J].Caryologia,2003，56(4):463-481.[15]张付远,李丹丹,廖忠明,等.不同消毒剂及激素对江西省野生白花凤仙花组培诱导的影响[J].现代农业科技,2015(15):164-165.[16]黄玉玲,朱跃珍,龙媛,等.美国车厘子组织培养试验[J].西南林业大学学报（自然科学）,2020,40(1):172-175.[17]热娜古丽·吐鲁洪,惠浩亮,刘甜甜,等.植物组织培养中褐变和玻璃化及污染的治理研究[J].黑龙江农业科学,2019(11):154-157.[18]邢东方,尚霄丽,冯建灿.枣组织培养中抗生素对污染的抑制作用[J].经济林研究,2010,28(1):26-29,68.[19]BARRETT C,CASSELLS A C.An evaluation of antibiotics for the elimination of Xanthomonas campestris pv.pelargonii (Brown) from Pelargonium x domesticum cv.‘Grand Slam’ explants in vitro[J]Plant Cell，Tissue and Organ Culture,1994,36(2):169-175.[20]王梅,汤浩茹,刘淑芳,等.4种抗生素对草莓“章姬”内生菌的抑制及对试管苗生长的影响[J].四川农业大学学报,2005,23(2):189-191.[21]SAMIEI L，PANHEHKOLAYI M D，MIRSHAHI H，et al.A simple and efficient micropropagation protocol for New Guinea Impatiens (Impatiens hawkeri)[J].Journal of Environmental Biology,2018，39(4):454-458.[22]张彦.新几内亚凤仙组培快繁技术研究[D].保定:河北农业大学,2006.[23]刘静,丁兰,赵庆芳,等.凤仙花的组织培养与离体快繁[J].西北师范大学学报(自然科学版),2008,44(1):80-82,87.[24]徐士清,杨世湖,倪丹,等.非洲菊试管苗叶片的组培快繁[J].园艺学报,2002,29(5):493-494,504.[25]郭燕华,罗丽萍,杨柏云,等.TDZ在新几内亚凤仙组织培养中的应用[C].福州：第二届全国植物组织培养、脱毒快繁及工厂化生产学术研讨会,2004.[26]曾文丹,严华兵,曹升,等.何首乌离体快繁技术体系的建立[J].南方农业学报,2018,49(12):2494-2499.[27]赵春晓,王春艳,苏秀红,等.一种卷丹高效快繁体系的建立[J].植物生理学报,2020,56(1):101-108.[28]彭文丽,李萃玲,朱靖杰,等.响应面法优化‘Duck7’油梨离体快繁技术的研究[J].植物生理学报,2019,55(8):1098-1108.
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备注/Memo
第一作者简介：闻永慧（1985-），女，硕士，实验师，现主要从事园林植物种质资源利用与创新等研究工作。E-mail:wenyonghui520@126.com.责任作者：黄海泉（1974-），男，博士，教授，现主要从事园林植物等研究工作。E-mail:haiquanl@163.com.基金项目：国家自然科学基金资助项目(32060364,32060366)；云南省重点研发计划资助项目(2018BB013)；云南省高校园林植物与观赏园艺科技创新团队资助项目（51700204）；云南省中青年学术和技术带头人培养资助项目(2015HB046，2018HB024)；云南省教育厅科学研究基金资助项目（2021J0174）。收稿日期：2020-10-09