TIAN Songqing,YUAN Ruyou,WEI Qinhua,et al.Tissue Culture and Root Tuber Induction of Dahlia pinnate‘Filogo’[J].Northern Horticulture,2020,44(07):79-85.[doi:10.11937/bfyy.20192453]
大丽花‘费罗加’组织培养和块根诱导
- Title:
- Tissue Culture and Root Tuber Induction of Dahlia pinnate‘Filogo’
- Keywords:
- Dahlia pinnate ‘Filogo’; stem segment; primary culture; proliferation culture; adventitious root and tuber induction
- 文献标志码:
- A
- 摘要:
- 以大丽花品种‘费罗加’为试材，茎段作为外植体，探讨了不同基本培养基、激素组合、蔗糖浓度、光照时间等对诱导愈伤组织、不定芽、不定根和块根的影响，以期缩短块根生产周期。结果表明：初代培养在MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+蔗糖30 g·L-1+琼脂6 g·L-1上愈伤组织诱导率为54.9%，褐化率为44.8%，污染率为26.6%，成活率达69.6%。继代增殖培养最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 0.01 mg·L-1+蔗糖30 g·L-1+琼脂6 g·L-1，增殖系数为7.8，愈伤组织诱导率22.9%。生根最佳培养基为WPM+NAA 1.0 mg·L-1+6-BA 0.1 mg·L-1+蔗糖60 g·L-1+琼脂6 g·L-1+炭粉2 g·L-1，最适宜光照时间为6 h，生根率为98.5%，根系密集健壮。块根诱导最适材料为带根瓶苗，出现初步形态的块根，形成块根率达56.8%。
- Abstract:
- The effects of different basic medium,hormone combinations,sucrose concentration and light duration on callus induction,adventitious bud induction,adventitious root induction and root tuber induction were studied by using Dahlia pinnate ‘Filogo’ stem segments as explants,in order shorten the production cycle of root tuber.The results showed that the callus induction rate,browning rate,contamination rate and survival rate were 54.9%,44.8%,26.6% and 69.6% respectively on MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+sucrose 30 g·L-1+agar 6 g·L-1.The optimum medium for subculture was MS+6-BA 2.0 mg·L-1+NAA 0.01 mg·L-1+sucrose 30 g·L-1+agar 6 g·L-1,the proliferation coefficient was 7.8,and the callus induction rate was 22.9%.The optimum medium for rooting was WPM+NAA 1.0 mg·L-1+6-BA 0.1 mg·L-1+sucrose 60 g·L-1+agar 6 g·L-1+carbon powder 2 g·L-1.The optimum illumination time was 6 hours per day,the rooting rate was 98.5%,and the roots were dense and robust.The optimum material for root induction was bottle seedling with roots.The rate of root tuber formation with initial morphology was 56.8%.
参考文献/References:
[1]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1979.[2]李仁娜,李艳,王宏,等.西安地区50种大丽花的园林观赏性评价[J].湖北农业科学,2019,58(3):68-72.[3]林萍.春色满园庭院花卉[M].北京:化学工业出版社,2009.[4]周永国,胡永利,班景昭.对大丽花块根开发利用的探究[J].国土与自然资源研究,1992(1):74-75.[5]袁震.大丽花离体快繁技术研究[D].南京:南京农业大学，2016.[6]李坚.苏州地区大丽花引种评价与应用研究[D].苏州:苏州大学,2017.[7]刘安成,王庆,庞长民.我国大丽花园艺学研究进展[J].北方园艺,2010(11):225-228.[8]杨增海.园艺植物组织培养[M].北京:农业出版社,1987.[9]韦三立,李颖章,韩碧文.大丽花的组织培养[J].植物生理学通讯,1996(6):428.[10]韦三立,李丕明,何钟佩,等.大丽花茎段的离体快速繁殖[J].北京农业大学学报,1990(4):381-385.[11]赵艳格,孙伟,苗秀洁,等.大丽花组织培养技术研究[J].园艺与种苗,2017(5):39-41.[12]曹明颜.大丽花离体快繁和再生体系的建立[D].保定:河北农业大学,2012.[13]曹明颜,陈段芬.大丽花叶片离体再生体系的建立[J].中国农学通报,2012,28(10):168-172.[14]杨婷.大丽花组织培养与脱毒技术研究[D].保定:河北农业大学,2014.[15]任桂红,陈杰,张方芳,等.大丽花组织培养及快繁技术研究[J].北华大学学报(自然科学版),2012,13(4):410-412.[16]陈晓光,丁艳锋,唐忠厚,等.氮肥施用量对甘薯产量和品质性状的影响[J].植物营养与肥料学报,2015,21(4):979-986.[17]杨育峰,张晓申,王慧瑜,等.氮肥施用量对甘薯生长、含氮量及产量的影响[J].河南农业科学,2015,44(3):52-55.[18]丁雪梅,苑兆和,冯立娟,等.不同氮素水平对大丽花形态指标及生理特性的影响[J].草业科学,2012,29(4):592-598.
相似文献/References:
[1]陈丽文,荣薏,何贵整.金钻蔓绿绒组培再生体系的建立[J].北方园艺,2012,36(01):120.
CHEN Li-wen,RONG Yi,HE Gui-zheng.Establishment of Tissue Culture Regeneration System for Philodendron con-go[J].Northern Horticulture,2012,36(07):120.
[2]关丽霞.牡丹沙培嫩茎段离体培养的初步研究[J].北方园艺,2012,36(04):74.
GUAN Li-xia.Preliminary Study on in Vitro Culture of Sand-cultured Tender Stem Segments of Paeonia suffruticosa[J].Northern Horticulture,2012,36(07):74.
[3]孙骏威,方晓峰,陈珍.不同植物生长调节剂对黄秋葵组织培养的影响[J].北方园艺,2012,36(07):139.
SUN Jun-wei,FANG Xiao-feng,CHEN Zhen.Effects of Plant Growth Regulator on Tissue Culture of Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.[J].Northern Horticulture,2012,36(07):139.
[4]董敬超.沙棘品种“实优1号”的组织培养技术[J].北方园艺,2012,36(19):127.
DONG Jing-chao.Study on Tissue Culture of Hippophae rhamnoides ‘Real gifted one’[J].Northern Horticulture,2012,36(07):127.
[5]叶飞,建德锋.“维多利亚”葡萄茎段离体培养技术研究[J].北方园艺,2012,36(19):149.
YE Fei,JIAN De-feng.Study on the Technology of Stem Segments Culture of ‘Victoria’ Grape in vitro[J].Northern Horticulture,2012,36(07):149.
[6]张洪岩,陈秀玲,李景富,等.番茄材料“0946”和“0949”再生体系的研究[J].北方园艺,2012,36(12):125.
ZHANGHong-yan,CHENXiu-ling,LIJing-fu,et al.StudyonRegenererationSystemofTomatoAccessions‘0946’and‘0949’[J].Northern Horticulture,2012,36(07):125.
[7]管艳,梁国平,李玲,等.星点木愈伤组织诱导及植株再生研究[J].北方园艺,2013,37(24):109.
GUAN Yan,LIANG Guo-ping,LI Ling,et al.Study on Callus Induction and Plantlet Regeneration of Dracaena godseffiana[J].Northern Horticulture,2013,37(07):109.
[8]周佳红,邢才华,曹慧,等.秋子梨茎段薄层细胞培养再生初探[J].北方园艺,2015,39(06):95.[doi:10.11937/bfyy.201506026]
ZHOU Jia-hong,XING Cai-hua,CAO Hui,et al.Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis[J].Northern Horticulture,2015,39(07):95.[doi:10.11937/bfyy.201506026]
[9]李 志 清,吴 桂 萍,鞠 志 新.大丽花观赏特征与品种分类分析[J].北方园艺,2010,34(05):0.[doi:10.11937/bfyy.201005039]
LI Zhi-qing,WU Gui -ping,JU Zhi -xin.Analysis of the Ornamental Features and Cultivar Classification on Dahlia pinnata[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.201005039]
[10]穆红梅,杜秀菊,曹兴,等.白凤菜组培快繁技术研究[J].北方园艺,2015,39(21):96.[doi:10.11937/bfyy.201521025]
MU Hongmei,DU Xiuju,CAO Xing,et al.Study on Tissue Culture and Rapid Propagation of Gynura formosana Kitam[J].Northern Horticulture,2015,39(07):96.[doi:10.11937/bfyy.201521025]
备注/Memo
第一作者简介：田松青（1974-），女，硕士，副教授，高级工程师，现主要从事观赏植物资源利用与繁育等研究工作。E-mail：tiansongqing@126.com.责任作者：朱旭东（1972-），男，博士，教授，现主要从事花卉育种和产业化等研究工作。E-mail：13862130525@163.com.基金项目：江苏省 ‘333工程’中青年学术带头人资助项目（BRA2018375）；江苏现代农业(花卉)产业技术体系建设资助项目（JATS[2019]365）；苏州农业职业技术学院科研创新团队建设资助项目（CXTD201801）。收稿日期：2019-09-10