LI Jiaozhuo,MIN Dedong,LI Zilong,et al.Arginase Involves in Methyl Jasmonate-induced Postharvest Tomato Fruit Resistance Against Gray Mold[J].Northern Horticulture,2021,(11):97-104.[doi:10.11937/bfyy.20203405]
精氨酸酶在茉莉酸甲酯介导采后番茄果实灰霉病抗性中的作用
- Title:
- Arginase Involves in Methyl Jasmonate-induced Postharvest Tomato Fruit Resistance Against Gray Mold
- Keywords:
- tomato fruit; methyl jasmonate; arginase; disease resistance
- 文献标志码:
- A
- 摘要:
- 以绿熟期番茄果实为试材，采用30 μmol?L-1精氨酸酶抑制剂Nω-羟基-nor-L-精氨酸（nor-NOHA）-35 kPa真空渗透结合0.05 mmol?L-1 茉莉酸甲酯（MeJA）熏蒸处理，研究了精氨酸酶在MeJA介导采后果实灰霉病抗性中的作用，以期阐明MeJA调控采后果实抗病性的机制。结果表明：MeJA处理能够显著提高贮藏前期过氧化氢含量，诱导超氧化物歧化酶、过氧化物酶和贮藏后期过氧化氢酶活性，有效抑制相对电导率和丙二醛含量升高，提高多酚氧化酶、苯丙氨酸解氨酶、几丁质酶和β-1,3-葡聚糖酶活性，促进总酚积累，抑制采后番茄果实发病率及病斑面积。但是（nor-NOHA+MeJA）处理显著抑制了MeJA对精氨酸酶活力的诱导效应，降低了MeJA介导采后番茄果实灰霉病抗病性的诱导效应。综上所述，精氨酸酶在MeJA介导采后番茄果实灰霉病抗性中发挥重要作用。
- Abstract:
- The tomato fruits of harvested at green-mature were used as test materials.The tomato fruits were fumigated with 0.05 mmol?L-1 methyl jasmonate (MeJA) after treated with 30 μmol?L-1 Nω-hydroxy-nor-L-arginine (nor-NOHA，an inhibitor of arginase) under low pressure (-35 kPa).The roles of arginase involved in MeJA-induced postharvest tomato fruit resistance against gray mold were studied,in order to explain the regulation mechanism of MeJA on postharvest disease resistance.The results showed that MeJA treatment largely promoted hydrogen peroxide content shortly after treatment，significantly induced activity of superoxide dismutase and peroxidase and significantly enhanced catalase activity during the later stages，and largely inhibited the enhancement of relative electrical conductivity and malondialdehyde.Meanwhile，MeJA treatment also induced the activities of phenylalanine ammonialyase，polyphenol oxidase，chitinase and β-1,3-glucanase，and promoted the accumulation of total phenolic.However，these effects of MeJA treatment were partially counteracted in fruit treated with (nor-NOHA+MeJA).Thus，the results suggested that arginase played important roles in MeJA-induced postharvest tomato fruit resistance against gray mold.
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备注/Memo
第一作者简介：李娇卓（1996-），女，硕士研究生，研究方向为果蔬采后生理与分子生物学。E-mail:11yaoyao96@sina.com.责任作者：张新华（1976-），女，博士，副教授，博士生导师，现主要从事果蔬采后生理与分子生物学等研究工作。E-mail：zxh@sdut.edu.cn.基金项目：国家自然科学基金资助项目（31772024）。收稿日期：2020-08-11