[1]秦文韬,谷彤彤,刘宇,等.杏鲍菇枯萎病菌PCR检测方法的建立与初步应用[J].北方园艺,2021,(07):123-128.[doi:10.11937/bfyy.20202726]
　QIN Wentao,GU Tongtong,LIU Yu,et al.Establishment and Preliminary Application of PCR Detection Methods for Pantoea pleuroti[J].Northern Horticulture,2021,(07):123-128.[doi:10.11937/bfyy.20202726]
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杏鲍菇枯萎病菌PCR检测方法的建立与初步应用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年07 页码: 123-128 栏目: 出版日期: 2021-04-15
Title:
Establishment and Preliminary Application of PCR Detection Methods for Pantoea pleuroti
作者:
秦文韬; 谷彤彤; 刘宇; 宋忠娟; 荣成博
（北京市农林科学院 植物保护环境保护研究所，北京市食用菌工程技术研究中心，农业农村部华北都市农业重点实验室，北京 100097）
Author(s):
QIN Wentao; GU Tongtong; LIU Yu; SONG Zhongjuan; RONG Chengbo
(Institute of Plant and Environment Protection，Beijing Academy of Agriculture and Forestry Sciences/Beijing Engineering Research Center for Edible Mushroom/Key Laboratory of Urban Agriculture (North China) by Ministry of Agriculture and Rural Affairs,Beijing 100097)
关键词:
杏鲍菇; 枯萎病; Pantoea pleuroti; 聚合酶链式反应; 检测
Keywords:
Pleurotus eryngii; blight disease; Pantoea pleuroti; polymerase chain reaction; detection
DOI:
10.11937/bfyy.20202726
文献标志码:
A
Abstract:
Pantoea pleuroti，blight disease pathogen of Pleurotus eryngii was used as the test material，PCR primers were designed，synthesized and screened after analyzing the sequencing results of P.pleuroti genome，and the detection effect was studied，so as to establishefficient PCR detection system for P.pleuroti and provide molecular basis for scientific control of blight disease of P.eryngii.The results showed that the specificity of K3143f/R and K37f/R primers were strong，only when P.pleuroti genomic DNA was used as template，the PCR products showed a specific band of 660 bp and 666 bp，respectively，while the genomic DNA of 15 strains of Pantoea，three pathogens strains of common edible fungi disease and distilled water (negative control) were used as template,the PCR products showed no band.The detection systems based on these two primer pairs were of high sensitivity and were not interfered by the tissue fluid of P.eryngii，which could detect the lowest 3.6 pg?μ L-1 P.pleuroti genomic DNA.Furthermore，at least 100 cfu P.pleuroti could be detected when P.pleuroti had been inoculated onto the fruiting bodies of P.eryngii for 48 hours.In addition，primer K3143F/R was of higher efficiency than that of K37F/R.

参考文献/References:

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备注/Memo

第一作者简介：秦文韬（1988-），女，博士，助理研究员，现主要从事食用菌病虫害综合防控等研究工作。E-mail：qinwentao@ipepbaafs.cn.责任作者：荣成博（1981-），女，博士，副研究员，现主要从事食用菌育种及栽培等研究工作。E-mail：woshiboer@163.com.基金项目：国家自然科学基金青年科学基金资助项目（31701975）；北京市农林科学院创新能力建设专项资助项目（KJCX20200105）。收稿日期：2020-06-29

更新日期/Last Update: 2021-06-28