[1]蒋素华,张燕,牛苏燕,等.蝴蝶兰切花花被不同开放阶段酶活性的变化[J].北方园艺,2021,(02):85-90.[doi:10.11937/bfyy.20200456]
　JIANG Suhua,ZHANG Yan,NIU Suyan,et al.Changes in Enzymatic Activity of Different Flowering Stages of Phalaenopsis aphrodite Cut-flower[J].Northern Horticulture,2021,(02):85-90.[doi:10.11937/bfyy.20200456]
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蝴蝶兰切花花被不同开放阶段酶活性的变化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年02 页码: 85-90 栏目: 出版日期: 2021-01-30
Title:
Changes in Enzymatic Activity of Different Flowering Stages of Phalaenopsis aphrodite Cut-flower
作者:
蒋素华1; 张燕1; 牛苏燕1; 杨旭东1; 王喜蒙2; 崔波1
（1.郑州师范学院 生物工程研究中心，河南 郑州 450044；2.郑州大学 生命科学学院，河南 郑州 450002）
Author(s):
JIANG Suhua1; ZHANG Yan1; NIU Suyan1; YANG Xudong1; WANG Ximeng2; CUI Bo1
(1.Institute of Bioengineering，Zhengzhou Normal University，Zhengzhou,Henan 450044；2.College of Life Sciences，Zhengzhou University，Zhengzhou,Henan 450002)
关键词:
蝴蝶兰; 过氧化物酶; 超氧化物歧化酶; 中性蛋白酶
Keywords:
Phalaenopsis aphrodite; peroxidase; superoxide dismutase; neutral protease
DOI:
10.11937/bfyy.20200456
文献标志码:
A
摘要:
以蝴蝶兰鲜切花为试材，对切花花被不同生理阶段3种酶活性的变化进行测量分析，以期为蝴蝶兰切花寿命的延长提供参考依据。结果表明：萼片和花瓣中的过氧化物酶（POD）从花苞期到半开期酶活性变化不明显，从半开期到衰老期酶活性显著升高。花瓣中的超氧化物歧化酶（SOD）从花苞期到半开期酶活性升高，从半开期到衰老期酶活性显著降低；萼片中的超氧化物歧化酶活性变化不显著但也呈先上升后下降趋势。花瓣中的中性蛋白酶（NP）从花苞期到半开期酶活性呈上升趋势，半开期到盛开期呈下降趋势，盛开期到衰老期呈上升趋势；花萼中的中性蛋白酶从花苞期到盛开期酶活性呈下降趋势，从盛开期到衰老期呈上升趋势；总体来看中性蛋白酶的活性呈先下降后上升趋势。
Abstract:
Taking fresh cut-flower of Phalaenopsis aphrodite as test materials,the changes in the activities of the three enzymes at different stages of the cut-flower tepals were measured and analyzed,in order to provide reference for prolonging the life span cut-flower of Phalaenopsis aphrodite.The results showed that the enzyme activity of peroxidase in sepals and petals did not change significantly from the bud stage to the half-blooming stage,and the enzyme activity increased significantly from the half-blooming stage to the senescence stage.The enzyme activity of superoxide dismutase in petals increased from the bud stage to the half-blooming stage,and the enzyme activity decreased significantly from the half-blooming stage to the senescence stage.The enzyme activity of neutral protease in the petals shows an upward trend from the bud stage to the half-blooming stage,a downward trend from the half-blooming stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.However,In the sepals the enzyme activity of the neutral protease showed a downward trend from the bud stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.Overall,the enzyme activity of neutral protease in both sepals and petals showed a first decline and then an upward trend.

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备注/Memo

第一作者简介：蒋素华(1983-)，女，硕士，副教授，现主要从事花卉分子生物学等研究工作。E-mail：jiangsuhua2006@163.com.责任作者：崔波（1962-），男，博士，教授，现主要从事植物分子育种等研究工作。E-mail：laocuibo@ 163.com.基金项目：河南省科技厅重大专项资助项目(701519,702056)；河南省科技厅科技发展计划资助项目(172107000003,172102110227)；河南省科技攻关资助项目（182102110357）。收稿日期：2020-02-10

更新日期/Last Update: 2021-05-19