SUN Ru-bin,YU Niu,SUN Pei-guang.Research Progress on Gene Engineering of Phalaenopsis Plants[J].Northern Horticulture,2014,38(12):177-180.
蝴蝶兰基因工程研究进展
- Title:
- Research Progress on Gene Engineering of Phalaenopsis Plants
- 文章编号:
- 1001-0009(2014)12-0177-04
- Keywords:
- Phalaenopsis; color gene; genetic engineering
- 分类号:
- S 682. 31
- 文献标志码:
- A
- 摘要:
- 蝴蝶兰是研究花的发育分化及着色机制的理想材料。蝴蝶兰查儿酮合成酶（CHS）基因、类黄酮3′,5′羟基化酶基因和ACC合成酶基因的分离和克隆为蝴蝶兰的花色机理和子房发育研究提供了基础。该文对蝴蝶兰的基因分离与克隆、蝴蝶兰以原球茎为材料采用基因枪法或农杆菌侵染法转化获得转基因植株等研究进展进行了综述，指出了蝴蝶兰基因工程研究主要集中在花色改良和抗病害方面，以期为蝴蝶兰基因工程研究提供参考。
- Abstract:
- Phalaenopsis is ideal for research on flower development and pigmentation.Chalcone synthase (CHS),Flavonoid3′,5′hydroxylase and ACC synthase gene have been identified.In this paper,Phalaenopsis gene isolation and cloning,Phalaenopsis bulbs for the materials used in the original research progress transforming gene transgenic plants such as particle bombardment or Agrobacterium infection method to get reviewed,improved color Phalaenopsis pointed out by engineering studies focused on and disease resistance,with a view to Phalaenopsis because engineering studies for reference.
参考文献/References:
[1] Tsai W C,Kuoh C S,Chuang M H,et al.Four DEF-Like MADS Box genes displayed distinct floral morphogenetic roles in Phalaenopsis orchid[J].Plant Cell Physiol,2004,45(7):831-844.
[2] Tsai W C,Lee P F,Chen H I,et al.PeMADS6,a GLOBOSA／PISTILLATA-like gene in Phalaenopsis equestris involved in petaloid formation,and correlated with flower longevity and ovary development[J].Plant Cell Physiol,2005,46(7):1125-1139.
[3] Tsai W C,Pan Z J,Hsiao Y Y,et al.Interactions of B-class complex proteins involved in tepal development in Phalaenopsis orchid[J].Plant Cell Physiol,2008,49(5):814-824.
[4] 郭滨,陈东红,戴薇,等.蝴蝶兰花发育相关基因pPI9的克隆与表达分析[J].复旦学报(自然科学版),2006(3):5-10.
[5] Chen D H,Bin G,Hexige S,et al.SQUA-like genes in the orchid Phalaenopsis are expressed in both vegetative and reproductive tissues[J].Planta,2007,226:369-380.
[6] 王玲,张宪省,钟诲文,等.蝶兰胚珠发育基因的表达及序列分析[J].植物学报,1999,41:276-279.
[7] Do Y Y,Huang P L.Characterization of a pollination-related cDNA from Phalaenopsis encoding a protein which is homologous to human peroxisomal acyl-CoA oxidase[J].Arch Biochem Biophys,1997,344(2):295-300.
[8] O’Neill S D,Nadeau J A,Zhang X S,et al.Interorgan regulation of ethylene biosynthetic genes by pollination[J].Plant Cell,1993(5):419-432.
[9] Bui A Q,O’Neill S D.Three l-aminocyclopropane-1-carboxylate synthase genes regulated by primary and secondary pollination signals in orchid flowers[J].Plant Physiol,1998,116:419-428.
[10] Wang N N,Chang Y Y.Differential expression of 1-aminocyclopropane-1 carboxylate synthase genes during orchid flower senescence induced by the protein phosphatase inhibitor okadaic acid[J].Plant Physiol,2001,126:253-260.
[11] 许华欣,黄鹏林.蝴蝶兰苯基苯乙烯酮合成酶eDNA之选殖及分析[J].中国园艺,1999,45:19-35.
[12] 韩颖颖,明风,王敬文,等.蝴蝶兰查儿酮合酶cDNA的克隆、鉴定及其原核表达[J].复旦学报(自然科学版),2004,43(2):235-239.
[13] Han Y Y,Ming F,Wang J W,et al.A novel chalcone synthase gene from Phalaenopsis orchid that alters floral morphology in transgenic tobacco plants[J].Plant Molecular Biology Reporter,2005,23:193.
[14] Su V,Hsu B D.Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers[J].Biotechnol Lett,2003,25(22):1933-1939.
[15] Wang J W,Ming F,Han Y Y,et al.Flavonoid-3′,5′-hydroxylase from Phalaenopsis:a novel member of cytochrome P450s,its cDNA cloning,endogenous expression and molecular modeling[J].Biotechnology Letters,2006,28:327-334.
[16] Ma H M,Pooler M.Anthocyanin regulatory/structural gene expression in Phalaenopsis[J].J Amer Soc Hort Sci,2009,134(1):88-96.
[17] Hsiao Y Y,Tsai W C,Kuoh C S,et al.Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway[J].BMC Plant Biology,2006(10):6-14.
[18] Anzai H,Ishii Y,Shichinohe M,et al.Transformation of Phalaenopsis byparticle bombardment[J].Plant Tissue Culture Letters,1996,13(3):265-272.
[19] Belarmino M M,Mii M.Agrobacteriummediated genetic transformation of a Phalanopsis orchid[J].Plant Cell Reports,2000,19:435-442.
[20] Chai M L,Xu C J,Senthil K K,et al.Stable transformation of protocorm-like bodies in Phalaenopsis orchid mediated by Agrobacterium tumefaciens[J].Scientia Horticulturae,2002,96:213-224.
[21] Mishiba K I,Chin D P,Mii M.Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at early stage after germination[J].Plant Cell Rep,2005,24:297-303.
[22] Liao L J,Pan I C,Chan Y L,et al.Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance[J].Molecular Breeding,2004,13:229-242.
[23] Chan Y L,Lin K H,Liao L J,et al.Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen Attack[J].Transgenic Research,2005,14:279-288.
[24] Rinaldi S,Dong P C,Raham S K,et al.Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing [1]wasabi defensin gene using Agrobacterium method[J].Plant Biotechnology,2006,23:191-194.
[25] Su V,Hsu B D.Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers[J].Biotechnology Letters,2003,25:1933-1939.
[26] Su V,Hsu B D.Transient expression of the cytochrome P450 CYP78A2 enhances anthocyanin production in flowers[J].Plant Mol Biol Rep,2010,28:302-308.
[27] 柴明良,金斗焕.农杆菌介导的蝴蝶兰基因转化系统的建立[J].园艺学报,2004,31(4):537-539.
[28] 满若君,卜朝阳,李杨瑞.蝴蝶兰?文心兰遗传转化体系的初步研究[J].安徽农业科学,2008,36（8):3129-3131.
相似文献/References:
[1]董平,赵培宝,任爱芝.蝴蝶兰灰霉病菌的分离鉴定[J].北方园艺,2014,38(04):109.
DONG Ping,ZHAO Pei-bao,REN Ai-zhi.Isolation and Identification of Botrytis cinerea?in Phalaenopsis[J].Northern Horticulture,2014,38(12):109.
[2]王常芸,李晓亮,张京伟,等.蝴蝶兰组培快繁技术体系研究[J].北方园艺,2013,37(15):122.
WANG Chang-yun,LI Xiao-liang,ZHANG Jing-wei,et al.Study on Tissue Culture and Rapid Propagation System of Phalaenopsis[J].Northern Horticulture,2013,37(12):122.
[3]丁兰,郭艳,董刚,等.国产蝴蝶兰种苗携带建兰花叶病毒(CymMV)和齿兰环斑病毒 (ORSV)的调查及脱毒的初步研究[J].北方园艺,2012,36(02):137.
DING Lan,GUO Yan,DONG Gang,et al.Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China[J].Northern Horticulture,2012,36(12):137.
[4]孔芳,凌宏龙,薛正莲,等.保鲜液对蝴蝶兰切花保鲜生理效应的研究[J].北方园艺,2013,37(02):130.
KONG Fang,LING Hong-long,XUE Zheng-lian,et al.Research on Preservatives of Cut Phalaenopsis Flower and Related Physiological Influence[J].Northern Horticulture,2013,37(12):130.
[5]景维杰,杨露露,蒋明殿.不同浓度氮、磷、钾对蝴蝶兰幼苗生长的影响[J].北方园艺,2012,36(23):62.
JING Wei-jie,YANG Lu-lu,JIANG Ming-dian.Effects of Different Concentrations of Nitrogen，Phosphorus and Potassium on the Growth of Phalaenopsis Seedlings[J].Northern Horticulture,2012,36(12):62.
[6]何荆洲,卜朝阳,黄昌艳,等.蝴蝶兰红花系品种“火凤凰”的杂交结实性研究[J].北方园艺,2015,39(02):61.[doi:10.11937/bfyy.201502017]
HE Jing-zhou,BU Zhao-yang,HUANG Chang-yan,et al.Study on Fecundity of Cross-pollination in Phalaenopsis ‘Fire Phoenix’[J].Northern Horticulture,2015,39(12):61.[doi:10.11937/bfyy.201502017]
[7]杜君,王利民,孟月娥,等.我国蝴蝶兰产业发展现状及对策研究[J].北方园艺,2015,39(09):172.[doi:10.11937/bfyy.201509047]
DU Jun,WANG Li-min,MENG Yue-e,et al.Study on the Development Status and Countermeasures of Phalaenopsis amabilis Industry in China[J].Northern Horticulture,2015,39(12):172.[doi:10.11937/bfyy.201509047]
[8]张 颖,郭 晓 东,王芳,等.蝴蝶兰组织培养快繁技术研究[J].北方园艺,2010,34(08):0.[doi:10.11937/bfyy.201008046]
ZHANG Ying,GUO Xiao -dong,W ANG Fang,et al.The Research on Tissue Culture and Rapid Propagation of Phalaenopsis Hybrid[J].Northern Horticulture,2010,34(12):0.[doi:10.11937/bfyy.201008046]
[9]王 桂 兰,陈 超,李 艳 梅,等.蝴蝶兰催花及开花过程中可溶性糖含量变化的研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010055]
W ANG Gui -lan,CHEN Chao,LI Yan -mei,et al.Study on Change of Soluble Sugar Content in Plant of Phalaenopsis During Flower Induction and Blooming Period[J].Northern Horticulture,2010,34(12):0.[doi:10.11937/bfyy.2010010055]
[10]许 传 俊,谭 茹 芳,陈 冬 茵,等.蝴蝶兰叶外植体发生褐变超微细胞结构和酚类物质分布观察[J].北方园艺,2010,34(21):0.[doi:10.11937/bfyy.201021031]
XU Chuan -jun,TAN Ru -fang,et al.Ultrastructure and Distribution of Phenol in Phalaenopsis Browning Leaf Explants[J].Northern Horticulture,2010,34(12):0.[doi:10.11937/bfyy.201021031]
备注/Memo
第一作者简介：孙汝斌（1983-），男，硕士研究生，助教，现主要从事园林植物和园林生态学的教学与科研工作。E-mail：sunrubin1@163.com.
基金项目：国家“十一五”科技支撑计划资助项目(2009BADB1B06)。
收稿日期：2014-03-03