[1]齐仙惠,王小军,李改珍,等.大白菜BrTFIIS基因的克隆及表达分析[J].北方园艺,2020,44(24):33-38.[doi:10.11937/bfyy.20200317]
　QI Xianhui,WANG Xiaojun,LI Gaizhen,et al.Cloning and Expression Analysis of BrTFIIS Gene in Chinese Cabbage[J].Northern Horticulture,2020,44(24):33-38.[doi:10.11937/bfyy.20200317]
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大白菜BrTFIIS基因的克隆及表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年24 页码: 33-38 栏目: 出版日期: 2020-12-30
Title:
Cloning and Expression Analysis of BrTFIIS Gene in Chinese Cabbage
作者:
齐仙惠1; 王小军2; 李改珍1; 巫东堂1; 赵军良1; 王秀英1
（1.山西省农业科学院 蔬菜研究所，山西 太原 030031；2.山西省蔬菜产业管理站，山西 太原 030002）
Author(s):
QI Xianhui1; WANG Xiaojun2; LI Gaizhen1; WU Dongtang1; ZHAO Junliang1; WANG Xiuying1
(1.Institute of Vegetables,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;2.Shanxi Vegetable Industry Management Station,Taiyuan,Shanxi 030002)
关键词:
大白菜; BrTFIIS; 基因克隆; 表达分析
Keywords:
Brassica rapapekinensis; BrTFIIS; gene cloning; expression analysis
DOI:
10.11937/bfyy.20200317
文献标志码:
A
摘要:
以大白菜茎尖生长点为研究材料，采用RT-PCR技术，克隆了大白菜BrTFIIS基因，并对其进行生物信息学分析和表达分析，以期为进一步研究大白菜的成花机理提供参考依据。结果表明：BrTFIIS的开放阅读框包含1 008 bp，可编码335个氨基酸；蛋白质分子质量为37.61 kDa，等电点为9.10；系统进化树分析表明BrTFIIS与甘蓝型油菜同源蛋白的亲缘关系最近。荧光定量PCR结果显示，BrTFIIS在大白菜营养生长和生殖生长阶段的茎尖生长点中均有表达，且在春化后表达量明显降低，并在随后的花芽分化进程中维持较低水平。推测BrTFIIS基因可能对大白菜的春化和花芽分化有抑制作用。
Abstract:
The stem tip growth point of Chinese cabbage was used as the research material,BrTFIIS gene was cloned by RT-PCR from the shoot apex of Chinese cabbage,and its bioinformatics analysis and expression analysis were carried out,in order to provide reference for further study on the flowering mechanism of Chinese cabbage.The results showed that the open reading frame of BrTFIIS contained 1 008 bp and encoded 335 amino acids.The molecular weight of protein was 37.61 kDa，and the isoelectric point was 9.10.Phylogenetic tree analysis indicated that BrTFIIS had the closest relationship with the homologous protein of Brassica napus.The results of real-time PCR showed that BrTFIIS was expressed in the shoot apex of Chinese cabbage at vegetative and reproductive stages.The expression decreased significantly after vernalization,and maintained a low level in the process of flower bud differentiation.BrTFIIS might have an inhibitory effect on vernalization and flower bud differentiation of Chinese cabbage.

参考文献/References:

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备注/Memo

第一作者简介：齐仙惠（1987-），女，博士，助理研究员，现主要从事蔬菜育种及生物技术应用等研究工作。E-mail：651345642@qq.com.责任作者：李改珍（1979-），女，博士，研究员，现主要从事蔬菜育种及生物技术应用等研究工作。E-mail：ggshx@126.com.基金项目：山西省重点研发计划重点资助项目（201703D211006）；山西省农业科学院博士研究基金资助项目（YBSJJ1712）；国家大宗蔬菜太原综合试验站资助项目（CARS-23-G-10）；山西省农业科学院攻关资助项目（yydzx01）。收稿日期：2020-01-22

更新日期/Last Update: 2021-03-26