[1]吴文能,李勇,雷霁卿,等.高通量测序技术对猕猴桃叶斑病微生物多样性研究[J].北方园艺,2020,44(20):21-26.[doi:10.11937/bfyy.20200262]
　WU Wenneng,LI Yong,LEI Jiqing,et al.Microbial Diversity of Kiwifruit Leaf Spot Disease by High-Throughput Sequencing[J].Northern Horticulture,2020,44(20):21-26.[doi:10.11937/bfyy.20200262]
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高通量测序技术对猕猴桃叶斑病微生物多样性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年20 页码: 21-26 栏目: 出版日期: 2020-10-30
Title:
Microbial Diversity of Kiwifruit Leaf Spot Disease by High-Throughput Sequencing
作者:
吴文能; 李勇; 雷霁卿; 王瑞
（贵阳学院 食品与制药工程学院，贵州 贵阳 550003）
Author(s):
WU Wenneng; LI Yong; LEI Jiqing; WANG Rui
(Food and Pharmaceutical Engineering Institute，Guiyang College，Guiyang,Guizhou 550003)
关键词:
高通量测序; 猕猴桃叶斑病; 多样性分析
Keywords:
high-throughput sequencing; kiwifruit leaf spot disease; diversity analysis
DOI:
10.11937/bfyy.20200262
文献标志码:
A
摘要:
以叶斑病猕猴桃叶片为试材，采用高通量测序方法，研究了猕猴桃叶斑病患病叶片中微生物群落结构，以期获得猕猴桃叶斑病的致病原因为针对性防治提供参考依据。结果表明：患病叶片中共检测到18 473个高质量细菌序列，包括14个细菌门、77个细菌属；以及43 229个高质量真菌序列，包括3个真菌门、145个真菌属。获得患病叶片的优势细菌假单胞菌属（Pseudomonas）及优势真菌种（Cercospora agavicola）。
Abstract:
The spot disease leaf of kiwifruit was used as material，the effect of microorganisms on leaf spot disease of kiwifruit was studied by high-throughput sequencing method.In order to provide research basis for the cause and targeted control of kiwifruit leaf spot disease.The results showed that total of 18 473 high-quality bacterial sequences were obtained from the diseased leaves，including 14 bacterial phylum and 77 bacterial genera；and 43 229 high-quality fungal sequences，including 3 fungal phylum and 145 fungal genera.Gain the dominant Pseudomonas of diseased leaves and the dominant fungal species Cercospora agavicola.

参考文献/References:

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备注/Memo

第一作者简介：吴文能（1983-），男，博士，副教授，现主要从事农作物病虫害综合防治等研究工作。E-mail:wuwenneng123@126.com.责任作者：王瑞（1979-），男，博士，教授，现主要从事农产品采后贮藏与保鲜等研究工作。E-mail:wangrui060729@126.com.基金项目：贵州省自然科技基金资助项目（黔科合基础[2016]1006）；贵州省农村产业革命水果产业发展专项资金资助项目（黔财农[2019]226号）；贵阳学院科研资金资助项目（GYU-KY-[2020]）。收稿日期：2020-01-17

更新日期/Last Update: 2020-12-30